[1]贾如江,侯丽艳,尹清臣,等.轴突诱导因子4D对人胰腺癌细胞增殖、迁移及血管生成的影响[J].新乡医学院学报,2017,34(12):1063-1067.[doi:10.7683/xxyxyxb.2017.12.005]
　JIA Ru-jiang,HOU Li-yan,YIN Qing-chen,et al.Effect of semaphorin 4D on the proliferation，migration and angiogenic of human pancreatic carcinoma cells[J].Journal of Xinxiang Medical University,2017,34(12):1063-1067.[doi:10.7683/xxyxyxb.2017.12.005]
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轴突诱导因子4D对人胰腺癌细胞增殖、迁移及血管生成的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
34
期数:
2017年12
页码:
1063-1067
栏目:
基础研究
出版日期:
2017-12-05

文章信息/Info

Title:
Effect of semaphorin 4D on the proliferation，migration and angiogenic of human pancreatic carcinoma cells
作者:
贾如江1; 侯丽艳1; 尹清臣1; 李丽芳2; 杨庚武1
（1.邯郸市中心医院普外一科，河北　邯郸　056102；2.邯郸市丛台区人民医院外科，河北　邯郸　056102）
Author(s):
JIA Ru-jiang1; HOU Li-yan1; YIN Qing-chen1; LI Li-fang2; YANG Geng-wu1
（1.Department of General Surgery，Handan Central Hospital，Handan 056102，Hebei Province，China；2.Department of Surgery，People′s Hospital of Handan city Congtai District，Handan 056102，Hebei Province，China）
关键词:
RNA干扰; 轴突诱导因子4D; 胰腺癌细胞; 增殖和迁移能力; 血管生成
Keywords:
RNA interference; semaphorin 4D; pancreatic carcinoma cells; proliferation and migration ability; angiogenesis
分类号:
R735.9
DOI:
10.7683/xxyxyxb.2017.12.005
文献标志码:
A
摘要:
目的　探讨siRNA沉默轴突诱导因子4D（Sema4D）对人胰腺癌细胞增殖、迁移及血管生成的影响。方法　设计合成Sema4D-siRNA，转染至人胰腺癌细胞系中，瞬时转染48 h后利用反转录-聚合酶链反应检测转染前后Sema4D mRNA表达的变化；瞬时转染72 h后利用Western blot法检测转染前后Sema4D蛋白表达的变化；采用四甲基偶氮唑蓝显色法观察转染后细胞生长变化；Transwell迁移实验、划痕修复实验检测转染后人胰腺癌细胞迁移性的改变；小管形成实验观察转染后人胰腺癌细胞培养上清液对血管形成能力的影响。结果　转染siRNA后，人胰腺癌细胞中Sema4D mRNA和Sema4D蛋白表达、胰腺癌细胞的生长速度均较阴性对照组和空白对照组下降（P<0.05）；Transwell迁移实验和划痕修复实验显示，胰腺癌细胞穿膜细胞数和划痕修复率显著低于阴性对照组和空白对照组（P<0.05）；小管形成实验显示，转染siRNA组、阴性对照组和空白对照组血管形成数目分别为0.50±0.02、1.45±0.60、1.37±0.52，组间比较差异有统计学意义（P<0.05）。结论　Sema4D-siRNA能够在胰腺癌细胞中引发RNA干扰效应，下调Sema4D基因表达，抑制胰腺癌细胞增殖，使胰腺癌细胞迁移能力明显下降，抑制血管生成。
Abstract:
Objective　To investigate the effects of semaphorin 4D(Sema4D) on the proliferation，migration and angiogenic of human pancreatic carcinoma cells.Methods　Sema4D-siRNA was designed and synthesized and transfected into human pancreatic carcinoma cells.After 48 hours of transient infection，the changes of expression of Sema4D mRNA before and after transfection were detected by reverse transcription-polymeruse chain reaction method.And after 72 hours of transient infection，the changes of expression of Sema4D protein before and after transfection were detected by Western blot method.The changes of growth of the transfected cells were observed by methyl thiazolyl terazolium assay.Using transwell migration test and scratch repair test to detect the changes of migration ability of human pancreatic carcinoma cells after transfection.Using tubule formation assay to observe the effect of supernatant of pancreatic carcinoma cell cultures on angiogenesis after transfection.Results　Compared with the negative control group and blank control group，the expression of Sema4D mRNA and Sema4D protein and the growth rate of pancreatic carcinoma cells decreased significantly （P<0.05）.In transwell migration test and scratch repair test，it was observed that Pancreatic cancer cells penetrating cell number and scratch repair rate were significantly lower than that in negative control group and blank control group（P<0.05）.Tubule formation assay showed that there were significant differences in angiogenesis numbers among siRNA transfection group（0.5±0.02），negative control group（1.45±0.60） and blank control group（1.37±0.52） （P<0.05）.Conclusion　Sema4D-siRNA can induce RNA interference in pancreatic carcinoma cells and down-regulate the expression of Sema4D gene，which can inhibit the proliferation of pancreatic carcinoma cells，significantly reduce the migration ability of pancreatic carcinoma cells and inhibit angiogenesis.

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更新日期/Last Update: 2017-12-05