[1]牛朝霞,陈　洁,彭蕤蕤,等.RNA干扰survivin基因对Eca-109细胞体内增殖的影响[J].新乡医学院学报,2016,33(5):352-355.[doi:10.7683/xxyxyxb.2016.05.002]
　NIU Zhao-xia,CHEN Jie,PENG Rui-rui,et al.Effect of silencing survivin gene by RNA interference on Eca-109 cell proliferation in vivo[J].Journal of Xinxiang Medical University,2016,33(5):352-355.[doi:10.7683/xxyxyxb.2016.05.002]
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RNA干扰survivin基因对Eca-109细胞体内增殖的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年5
页码:
352-355
栏目:
基础研究
出版日期:
2016-05-05

文章信息/Info

Title:
Effect of silencing survivin gene by RNA interference on Eca-109 cell proliferation in vivo
作者:
牛朝霞; 陈　洁; 彭蕤蕤; 秦紫芳
（河南医学高等专科学校病理生理学教研室，河南　郑州　451191）
Author(s):
NIU Zhao-xia; CHEN Jie; PENG Rui-rui; QIN Zi-fang
(Department of Pathophysiology，Henan Medical College，Zhengzhou 451191，Henan Province，China)
关键词:
RNA干扰; survivin基因; Eca-109细胞; 细胞增殖; 食管癌
Keywords:
RNA interference; survivin gene; Eca-109 cell; cell proliferation; esophageal cancer
分类号:
R735.1
DOI:
10.7683/xxyxyxb.2016.05.002
文献标志码:
A
摘要:
目的　利用RNA干扰抑制Eca-109 细胞survivin基因表达，观察survivin沉默后对食管癌细胞Eca-109体内增殖的影响。方法　构建靶向survivin基因的小干扰RNA（siRNA）真核表达载体，利用LipofectamineTM 2000脂质体转染Eca-109细胞，建立稳定转染干扰组细胞Eca-109/si-survivin；同法建立阴性对照组细胞Eca-109/si-control，并以Eca-109为空白对照组细胞；通过Western blot检测survivin基因沉默效果；借助裸鼠移植瘤模型分析survivin干扰前后裸鼠成瘤时间及瘤结节质量的改变，比较各组Eca-109细胞的体内增殖速度；通过末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记技术法检测移植瘤细胞凋亡的变化。结果　干扰组细胞survivin蛋白表达显著低于阴性对照组和空白对照组（P<0.05），而阴性对照组细胞survivin蛋白表达与空白对照组比较差异无统计学意义（P>0.05)。干扰组裸鼠平均瘤结节质量显著低于阴性对照组和空白对照组（P<0.05），而阴性对照组与空白对照组裸鼠的平均瘤结节质量差异均无统计学意义（P>0.05）。干扰组裸鼠移植瘤细胞凋亡指数明显高于阴性对照组和空白对照组（P<0.05），而阴性对照组与空白对照组裸鼠移植瘤细胞凋亡指数比较差异无统计学意义（P>0.05）。结论　靶向survivin的siRNA能特异性沉默survivin基因表达，诱导细胞凋亡，进而抑制人食管癌Eca-109细胞的体内增殖。
Abstract:
Objective　To inhibit survivin gene expression by RNA interference technology and investigate the effect of silent survivin on Eca-109 cell proliferation in vivo.Methods　Small interfering RNA（SiRNA） eukaryotic expressing vector targeting survivin was constructed and transfected into Eca-109 cells via LipofectamineTM 2000 to establish stable transfection cell line Eca-109/si-survivin；the same method was used to establish the negative control group Eca-109/si-control and Eca-109 was used to be the blank control group；the expression level of survivin protein was detected by Western blot to observe the interference effect；the models of transplanted tumors in nude mice were constructed to analyze the time of tumor formation and the weight of tumor nodules and to compare cell proliferation in vivo of the different Eca-109 cells；cell apoptosis of the transplanted tumors was measured by terminal-deoxynucleotidyl transferase mediated nick end labeling （TUNEL） assay.Results　The protein level of survivin interference group was effectively lower than that of the negative and blank control groups （P<0.05），but there was no significant difference between the negative group and the blank control group(P>0.05).The mean weight of tumor nodules in nude mice of the survivin interference group was evidently lower than that of the negative and blank control groups(P<0.05)，but there was no significant difference between the negative group and the blank control group(P>0.05).With the finding that cell apoptosis index(AI) of transplanted tumor in nude mice in the survivin interference group was obviously higher than that of the control groups(P<0.05)，but there was no significant difference between the negative group and the blank control group(P>0.05).Conclusion　SiRNA targeting survivin can specifically silence the expression of survivin gene，induce cell apoptosis，and thus inhibit cell proliferation in vivo of human esophageal cancer Eca-109 cells.

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更新日期/Last Update: 2016-05-05