[1]赵二趁,崔　静,冯思佳,等.磷脂酸磷酸酶2域1A基因在乳腺上皮细胞和乳腺癌细胞中的表达[J].新乡医学院学报,2016,33(5):356-358.[doi:10.7683/xxyxyxb.2016.05.003]
　ZHAO Er-chen,CUI Jing,FENG Si-jia,et al.Expression of phosphatidic acid phosphatase type 2 domain containing 1A gene in mammary epithelial cells and breast carcinoma cells[J].Journal of Xinxiang Medical University,2016,33(5):356-358.[doi:10.7683/xxyxyxb.2016.05.003]
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磷脂酸磷酸酶2域1A基因在乳腺上皮细胞和乳腺癌细胞中的表达
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年5
页码:
356-358
栏目:
基础研究
出版日期:
2016-05-05

文章信息/Info

Title:
Expression of phosphatidic acid phosphatase type 2 domain containing 1A gene in mammary epithelial cells and breast carcinoma cells
作者:
赵二趁1; 2; 崔　静1; 冯思佳1; 杨　璐1; 柴　婕1; 杨丹丹1; 张翠翠1; 王志慧1; 郜培琼1; 李　娜1; 千新来1
（1.新乡医学院基础医学院病理学教研室，河南　新乡　453003;2.周口市中心医院病理科，河南　周口　466000）
Author(s):
ZHAO Er-chen1; 2; CUI Jing1; FENG Si-jia1; YANG Lu1; CHAI Jie1; YANG Dan-dan1; ZHANG Cui-cui1; WANG Zhi-hui1; GAO Pei-qiong1; LI Na1; QIAN Xin-lai1
（1.Department of Pathology，School of Basic Medical Sciences，Xinxiang Medical University，Xinxiang 453003，Henan Province，China;2.Department of Pathology,Zhoukou Central Hospital,Zhoukou 466000,Henan Province,China）
关键词:
乳腺癌; 磷脂酸磷酸酶2域1A基因; 信使RNA; 蛋白
Keywords:
breast carcinoma; phosphatidic acid phosphatase type 2 domain containing 1A; messenger RNA; protein
分类号:
R737.9
DOI:
10.7683/xxyxyxb.2016.05.003
文献标志码:
A
摘要:
目的　探讨磷脂酸磷酸酶2域1A（PPAPDC1A）基因在正常乳腺上皮细胞和不同转移能力的乳腺癌细胞中表达的差异。方法　以正常乳腺上皮HBL-100细胞、低转移乳腺癌MCF-7细胞、高转移乳腺癌MDA-MB-435s细胞和高转移乳腺癌MDA-MB-231细胞为研究对象，通过实时荧光定量聚合酶链反应（qPCR）和Western blot法分别检测PPAPDC1A基因mRNA和蛋白在上述细胞中的表达。结果　与正常乳腺上皮HBL-100细胞比较，3种不同转移能力的乳腺癌细胞中的PPAPDC1A mRNA和蛋白表达水平均升高（P<0.01），且高转移乳腺癌MDA-MB-231细胞和高转移乳腺癌MDA-MB-435s细胞中PPAPDC1A mRNA和蛋白表达水平均高于低转移乳腺癌MCF-7细胞（P<0.01），高转移乳腺癌MDA-MB-231细胞中PPAPDC1A mRNA和蛋白水平的表达高于高转移乳腺癌MDA-MB-435s细胞（P<0.01）。PPAPDC1A在4种细胞中表达的顺序为HBL-100细胞<MCF-7细胞<MDA-MB-435s细胞<MDA-MB-231细胞。结论　PPAPDC1A基因可能促进了乳腺癌的发生、转移。
Abstract:
Objective　To investigate the expression difference of phosphatidic acid phosphatase type 2 domain containing 1A（PPAPDC1A）gene in normal mammary epithelial cells and breast carcinoma cells with different metastatic abilities.Methods　The mRNA and protein expressions of PPAPDC1A gene in normal mammary epithelial HBL-100 cells，low metastatic breast carcinoma MCF-7cells，high metastatic breast carcinoma MDA-MB-231 cells and MDA-MB-435s cells were detected by real-time quantitative PCR(qPCR) and Western blot assay，respectively.Results　The qPCR and Western blot results showed that，compared with normal mammary epithelial HBL-100 cells，the mRNA and protein expressions of PPAPDC1A gene in three different metastatic ability of breast carcinoma cells were significantly increased(P<0.01).Furthermore，the mRNA and protein expressions of PPAPDC1A gene in high metastatic breast carcinoma MDA-MB-231 cells and MDA-MB-435s cells were significantly higher than those in the low metastatic breast carcinoma MCF-7 cells(P<0.01)，and the mRNA and protein expressions of PPAPDC1A gene in high metastatic breast carcinoma MDA-MB-231 cells were significantly higher than those in the high metastatic breast carcinoma MDA-MB-435s cells(P<0.01).The expression order of PPAPDC1A gene in four kinds of cells was as follows：HBL-100 cells<MCF-7 cells<MDA-MB-435s cells<MDA-MB-231 cells.Conclusion　The PPAPDC1A gene may promote the occurrence and metastasis of breast carcinoma.

参考文献/References:

［1］　WAGGONER D W，XU J，SINGH I，et al.Structural organization of mammalian lipid phosphate phosphatases：implications for signal transduction［J］.Biochim Biophys Acta，1999，1439(2)：299-316.
［2］　KANOH H，KAI M，WADA I.Phosphatidic acid phosphatase from mammalian tissues：discovery of channel-like proteins with unexpected functions［J］.Biochim Biophys Acta，1997，1348(1/2)：56-62.
［3］　SCIORRA V A，MORRIS A J.Roles for lipid phosphate phosphatases in regulation of cellular signaling［J］.Biochim Biophys Acta，2002，1582(1/2/3)：45-51.
［4］　王慧.乳腺癌干细胞标志物及乳腺癌干细胞治疗策略［J］.新乡医学院学报，2015，32（10）：964-967.
［5］　WOOLSTON C.Breast cancer：4 big questions［J］.Nature，2015，527(7578)：S120.
［6］　QUIROGA-GARCíA V，CIRAUQUI-CIRAUQUI B，BUGéS-SáNCHEZ C，et al.Primary hormone therapy in elderly women with hormone-sensitive locoregional breast cancer：endocrine therapy alone is a reasonable alternative in selected patients［J］.Breast Care(Basel)，2015，10(3)：179-183.
［7］　COUGHLIN S S，SMITH S A.The Impact of the natural，social，built，and policy environments on breast cancer［J］.J Environ Health Sci，2015，1(3)：1-7.
［8］　ENGEL C，FISCHER C.Breast cancer risks and risk prediction models［J］.Breast Care(Basel)，2015，10(1)：7-12.
［9］　AUPPERLEE M D，ZHAO Y，TAN Y S，et al.Puberty-specific promotion of mammary tumorigenesis by a high animal fat diet［J］.Breast Cancer Res，2015，17(1)：138-158.
［10］　BENJAMIN D I，LI D S，LOWE W，et al.Diacylglycerol metabolism and signaling is a driving force underlying FASN inhibitor sensitivity in cancer cells［J］.ACS Chem Biol，2015，10(7)：1616-1623.
［11］　BRINDLEY D N，PILQUIL C.Lipid phosphate phosphatases and signaling［J］.J Lipid Res，2009，50（Suppl）：S225-S230.
［12］　TAKEUCHI M，HARIGAI M，MOMOHARA S，et al.Cloning and characterization of DPPL1 and DPPL2，representatives of a novel type of mammalian phosphatidate phosphatase［J］.Gene，2007，399(2)：174-180.
［13］　TANG X，BENESCH M G，BRINDLEY D N.Lipid phosphate pho-sphatases and their roles in mammalian physiology and pathology［J］.J Lipid Res，2015，56(11)：2048-2060.
［14］　DAHL E，KRISTIANSEN G，GOTTLOB K，et al.Molecular profiling of laser-microdissected matched tumor and normal breast tissue identifies karyopherin alpha2 as a potential novel prognostic marker in breast cancer［J］.Clin Cancer Res，2006，12(13)：3950-3960.
［15］　MANZANO R G，MARTINEZ-NAVARRO E M，FORTEZA J，et al.Microarray phosphatome profiling of breast cancer patients unveils a complex phosphatase regulatory role of the MAPK and PI3K pathways in estrogen receptor-negative breast cancers［J］.Int J Oncol，2014，45(6)：2250-2266.

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更新日期/Last Update: 2016-05-05