[1]柴　婕,冯思佳,崔　静,等.磷脂酸磷酸酶2域1A在不同转移能力肝癌细胞系中的表达[J].新乡医学院学报,2017,34(8):678-681.[doi:10.7683/xxyxyxb.2017.08.004]
　CHAI Jie,FENG Si-jia,CUI Jing,et al.Expression of phosphatidic acid phosphatase type 2 domain containing 1A in hepatocellular carcinoma cell lines with different metastatic ability[J].Journal of Xinxiang Medical University,2017,34(8):678-681.[doi:10.7683/xxyxyxb.2017.08.004]
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磷脂酸磷酸酶2域1A在不同转移能力肝癌细胞系中的表达
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
34
期数:
2017年8
页码:
678-681
栏目:
基础研究
出版日期:
2017-08-05

文章信息/Info

Title:
Expression of phosphatidic acid phosphatase type 2 domain containing 1A in hepatocellular carcinoma cell lines with different metastatic ability
作者:
柴　婕1; 冯思佳2; 崔　静1; 杨丹丹1; 张翠翠1; 郜培琼1; 王志慧1; 范　蕊1; 张向楠1; 王阳林1; 千新来1; 2
（1.新乡医学院基础医学院病理学教研室，河南　新乡　453003；2.新乡医学院第三附属医院病理科，河南　新乡　453003）
Author(s):
CHAI Jie1; FENG Si-jia2; CUI Jing1; YANG Dan-dan1; ZHANG Cui-cui1; GAO Pei-qiong1; WANG Zhi-hui1; FAN Rui1; ZHANG Xiang-nan1; WANG Yang-lin1; QIAN Xin-lai1; 2
(1.Department of Pathology,School of Basic Medical Sciences,Xinxiang Medical University,Xinxiang 453003,Henan Province,China;2.Department of Pathology,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003,Henan Province,China)
关键词:
肝细胞癌; 磷脂酸磷酸酶2域1A基因; 蛋白; 信使RNA
Keywords:
hepatocellular carcinoma; phosphatidic acid phosphatase type 2 domain containing 1A; protein; messenger RNA
分类号:
R735.7
DOI:
10.7683/xxyxyxb.2017.08.004
文献标志码:
A
摘要:
目的　研究磷脂酸磷酸酶2域1A（PPAPDC1A）在7种不同转移能力的肝癌细胞系中表达的差异。方法　以7种不同转移能力的肝癌细胞系（BEL-7402、HepG2、MHCC-97L、SMMC-7721、HCCL-M6、BEL-7404、MHCC-97H细胞）为研究对象，通过实时荧光定量聚合酶链式反应和Western blot法分别检测其PPAPDC1A mRNA和蛋白的表达。结果　在7种不同转移能力的肝癌细胞中，PPAPDC1A mRNA与PPAPDC1A蛋白的表达从低到高依次为BEL-7402细胞<HepG2细胞<MHCC-97L细胞<SMMC-7721细胞<HCCL-M6 细胞<BEL-7404细胞<MHCC-97H细胞；7种不同转移能力肝癌细胞中PPAPDC1A mRNA和PPAPDC1A蛋白的表达比较差异均有统计学意义（F=38.373、441.937，P<0.05）。其中HepG2细胞中PPAPDC1A蛋白的表达显著高于BEL-7402细胞（P<0.05）；MHCC-97L细胞中PPAPDC1A蛋白的表达显著高于HepG2细胞（P<0.05）；SMCC-7721、HCCL-M6细胞中PPAPDC1A蛋白的表达显著高于MHCC-97L细胞（P<0.05）；HCCL-M6细胞中PPAPDC1A蛋白的表达显著高于SMCC-7721细胞（P<0.05）；MHCC-97L、SMCC-7721、HCCL-M6、BEL-7404、MHCC-97H细胞中PPAPDC1A mRNA和PPAPDC1A 蛋白的表达显著高于BEL-7402细胞（P<0.05）；SMCC-7721、HCCL-M6、BEL-7404、MHCC-97H细胞中PPAPDC1A mRNA和PPAPDC1A 蛋白的表达显著高于HepG2 细胞（P<0.05）；BEL-7404、MHCC-97H细胞中PPAPDC1A mRNA和PPAPDC1A 蛋白的表达显著高于MHCC-97L细胞（P<0.05）；BEL-7404、MHCC-97H细胞中PPAPDC1A mRNA和PPAPDC1A 蛋白的表达显著高于SMCC-7721细胞（P<0.05）；BEL-7404、MHCC-97H细胞中PPAPDC1A mRNA和PPAPDC1A 蛋白的表达显著高于HCCL-M6细胞（P<0.05）；MHCC-97H细胞中PPAPDC1A mRNA和PPAPDC1A 蛋白的表达显著高于BEL-7404细胞（P<0.05）。结论　PPAPDC1A可能促进肝细胞癌的侵袭与转移。
Abstract:
Objective　To investigate the difference in the expression of phosphatidic acid phosphatase type 2 domain containing 1A (PPAPDC1A) in hepatocellular carcinoma cell lines with different metastatic ability.Methods　The expression of PPAPDC1A protein and mRNA in seven hepatocellular carcinoma cell lines(BEL-7402,HepG2,MHCC-97L,SMMC-7721,HCCL-M6,BEL-7404,MHCC-97H cell) with different metastatic ability was detected by Western blot and real-time fluorescent quantitative polymerase chain reaction,respectively.Results　The expression order of PPAPDC1A mRNA and PPAPDC1A protein in the seven different metastatic hepatocellular carcinoma cells was BEL-7402 cell <HepG2 cell <MHCC-97L cell <SMMC-7721 cell <HCCL-M6 cell <BEL-7404 Cell <MHCC-97H cell.There was significant difference in the expression of PPAPDC1A mRNA and PPAPDC1A protein in the seven liver cancer cells with different metastatic ability (F=38.373,441.937;P<0.05).The expression of PPAPDC1A protein in HepG2 cell was significantly higher than that in BEL-7402 cell (P<0.05).The expression of PPAPDC1A protein in MHCC-97L cell was significantly higher than that in HepG2 cell (P<0.05).The expression of PPAPDC1A protein in SMCC-7721 cell and HCCL-M6 cell was significantly higher than that in MHCC-97L cell (P<0.05).The expression of PPAPDC1A protein in HCCL-M6 cell was significantly higher than that in SMCC-7721 cell (P<0.05).The expression of PPAPDC1A mRNA and PPAPDC1A protein in MHCC-97L,SMCC-7721,HCCL-M6,BEL-7404 and MHCC-97H cell was significantly higher than that in BEL-7402 cell (P<0.05).The expression of PPAPDC1A mRNA and PPAPDC1A protein in SMCC-7721,HCCL-M6,BEL-7404l and MHCC-97H cell was significantly higher than that in HepG2 cell (P<0.05).The expression of PPAPDC1A mRNA and PPAPDC1A protein in BEL-7404 and MHCC-97H cell was significantly higher than that in MHCC-97L cell (P<0.05).The expression of PPAPDC1A mRNA and PPAPDC1A protein in BEL-7404 and MHCC-97H cell was significantly higher than that in SMCC-7721 cell (P<0.05).The expression of PPAPDC1A mRNA and PPAPDC1A protein in BEL-7404 and MHCC-97H cell was significantly higher than that in SMCC-7721 cell (P<0.05).The expression of PPAPDC1A mRNA and PPAPDC1A protein in BEL-7404 and MHCC-97H cell was significantly higher than that in HCCL-M6 cell (P<0.05).The expression of PPAPDC1A mRNA and PPAPDC1A protein in MHCC-97H cell was significantly higher than that in BEL-7404 cell (P<0.05).Conclusion　PPAPDC1A may promote the invasion and metastasis of hepatocellular carcinoma.

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更新日期/Last Update: 2017-08-05