[1]郜培琼,贺国洋,冯思佳,等.磷脂酸磷酸酶2域1A在不同结直肠癌细胞中的表达[J].新乡医学院学报,2018,35(3):163-166.[doi:10.7683/xxyxyxb.2018.03.001]
　GAO Pei-qiong,HE Guo-yang,FENG Si-jia,et al.Expression of phosphatidic acid phosphatase type 2 domain containing 1A in different human colorectal cancer cells[J].Journal of Xinxiang Medical University,2018,35(3):163-166.[doi:10.7683/xxyxyxb.2018.03.001]
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磷脂酸磷酸酶2域1A在不同结直肠癌细胞中的表达
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
35
期数:
2018年3
页码:
163-166
栏目:
基础研究
出版日期:
2018-03-05

文章信息/Info

Title:
Expression of phosphatidic acid phosphatase type 2 domain containing 1A in different human colorectal cancer cells
作者:
郜培琼1; 贺国洋1; 冯思佳2; 张翠翠1; 王志慧1; 范　蕊1; 王阳林1; 张向楠2; 赵　飞2; 王贝玺2; 千新来1; 原志庆1
（1.新乡医学院基础医学院病理学教研室，河南　新乡　453003；2.新乡医学院第三附属医院病理科，河南　新乡　453003）
Author(s):
GAO Pei-qiong1; HE Guo-yang1; FENG Si-jia2; ZHANG Cui-cui1; WANG Zhi-hui1; FAN Rui1; WANG Yang-lin1; ZHANG Xiang-nan2; ZHAO Fei1; WANG Bei-xi2; QIAN Xin-lai1; YUAN Zhi-qing1
（1.Department of Pathology，School of Basic Medical Sciences，Xinxiang Medical University，Xinxiang 453003，Henan Province，China；2.Department of Pathology，the Third Affiliated Hospital of Xinxiang Medical University，Xinxiang 453003，Henan Province，China）
关键词:
结直肠癌; 磷脂酸磷酸酶2域1A基因; mRNA; 蛋白
Keywords:
colorectal cancer; phosphatidic acid phosphatase type 2 domain containing 1A; mRNA
分类号:
R735.3
DOI:
10.7683/xxyxyxb.2018.03.001
文献标志码:
A
摘要:
目的　探讨磷脂酸磷酸酶2域1A（PPAPDC1A）在人结直肠癌细胞中的表达及意义。方法　取结直肠癌细胞系高转移潜能细胞LOVO、SW620和低转移潜能细胞SW480、RKO、HCT116、DLD-1进行常规培养，待细胞生长至对数生长期时，采用实时荧光定量聚合酶链反应检测不同结直肠癌细胞中PPAPDC1A mRNA表达，Western blot检测不同结直肠癌细胞中PPAPDC1A蛋白表达。结果　6种人结直肠癌细胞中PPAPDC1A mRNA和蛋白表达比较差异均有统计学意义（F=41.213、344.116，P<0.05）。高转移潜能细胞LOVO、SW620中PPAPDC1A mRNA和蛋白表达显著高于DLD-1、HCT116、RKO、SW480细胞（P<0.05）；高转移潜能细胞LOVO中PPAPDC1A蛋白表达显著高于SW620细胞（P<0.05）；DLD-1细胞中PPAPDC1A蛋白表达显著高于HCT116、RKO、SW480细胞（P<0.05）；低转移潜能细胞HCT116中PPAPDC1A蛋白表达显著高于RKO、SW480细胞（P<0.05）；RKO细胞中PPAPDC1A蛋白表达显著高于SW480细胞（P<0.05）。高转移潜能细胞LOVO与SW620细胞中PPAPDC1A mRNA表达比较差异无统计学意义（P>0.05）；SW480、RKO、HCT116、DLD-1细胞中PPAPDC1A mRNA表达两两比较差异均无统计学意义（P>0.05）。结论　PPAPDC1A在结直肠癌细胞系中存在差异性表达，其可能与结直肠癌的侵袭和转移有关。
Abstract:
Objective　To investigate the significance of the expression of phosphatidic acid phosphatase type 2 domain containing 1A（PPAPDC1A） in human colorectal cancer cell lines.Methods　The high metastatic potential cells LOVO,SW620 and low metastatic potential cells SW480，RKO，HCT116 and DLD-1 were cultured，the expression of PPAPDC1A mRNA and protein in different colorectal cancer cells in logarithmic growth period was detected by real-time quantitative polymerase chain reaction and Western blot.Results　There were significant differences in the expressions of PPAPDC1A mRNA and protein among the six human colorectal cancer cells （F=41.213，344.116；P<0.05）.The expression of PPAPDC1A mRNA and protein in highly metastatic potential cells LOVO and SW620 was significantly higher than that in DLD-1，HCT116，RKO and SW480 cells （P<0.05）.The expression of PPAPDC1A protein in LOVO cells with high metastatic potential was significantly higher than that in SW620 cells(P<0.05).The expression of PPAPDC1A protein in DLD-1 cells was significantly higher than that in HCT116，RKO and SW480 cells （P<0.05）.The expression of PPAPDC1A protein in HCT116 cells with low metastatic potential was significantly higher than that in RKO and SW480 cells （P<0.05）.The expression of PPAPDC1A protein in RKO cells was significantly higher than that in SW480 cells （P<0.05）.There was no significant difference in the expression of PPAPDC1A mRNA between LOVO and SW620 cells （P> 0.05）.There was no significant difference in the expression of PPAPDC1A mRNA between SW480，RKO，HCT116 and DLD-1 cells （P> 0.05）.Conclusion　PPAPDC1A expresses differentially in colorectal cancer cell lines，which may be involved in the invasion and metastasis of colorectal cancer.

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更新日期/Last Update: 2018-03-05