[1]杨丹丹,冯思佳,崔　静,等.磷脂酸磷酸酶2域1A在不同子宫颈癌细胞中的表达[J].新乡医学院学报,2017,34(8):674-677.[doi:10.7683/xxyxyxb.2017.08.003]
　YANG Dan-dan,FENG Si-jia,CUI jing,et al.Expression of phosphatidic acid phosphatase type 2 domain containing 1A in different human cervical carcinoma cells[J].Journal of Xinxiang Medical University,2017,34(8):674-677.[doi:10.7683/xxyxyxb.2017.08.003]
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磷脂酸磷酸酶2域1A在不同子宫颈癌细胞中的表达
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
34
期数:
2017年8
页码:
674-677
栏目:
基础研究
出版日期:
2017-08-05

文章信息/Info

Title:
Expression of phosphatidic acid phosphatase type 2 domain containing 1A in different human cervical carcinoma cells
作者:
杨丹丹1; 冯思佳2; 崔　静1; 柴　婕1; 张翠翠1; 郜培琼1; 王志慧1; 范　蕊1; 张向楠2; 王阳林1; 原志庆1
(1.新乡医学院基础医学院病理学教研室，河南　新乡　453003；2.新乡医学院第三附属医院病理科，河南　新乡　453003)
Author(s):
YANG Dan-dan1; FENG Si-jia2; CUI jing1; CHAI Jie1; ZHANG Cui-cui1; GAO Pei-qiong1; WANG Zhi-hui1; FAN Rui1; ZHANG Xiang-nan2; WANG Yang-lin1; YUAN Zhi-qing1
(1.Department of Pathology,College of Basic Medicine,Xinxiang Medical University,Xinxiang 453003，Henan Province,China;2.Department of Pathology,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003,Henan Province,China)
关键词:
子宫颈癌; 磷脂酸磷酸酶2域1A基因; 信使RNA
Keywords:
cervical carcinoma; phosphatidic acid phosphatase type 2 domain containing 1A gene; messenger RNA
分类号:
R737.33
DOI:
10.7683/xxyxyxb.2017.08.003
文献标志码:
A
摘要:
目的　探讨磷脂酸磷酸酶2域1A（PPAPDC1A）在人4种子宫颈癌细胞中表达的差异。方法　通过实时荧光定量聚合酶链式反应和Western blot法分别检测PPAPDC1A mRNA和蛋白在人子宫颈癌C33-A细胞、Caski细胞、HeLa细胞和SiHa细胞中的表达。结果　子宫颈癌SiHa、HeLa、Caski和C33-A细胞中PPAPDC1A mRNA的表达量分别为 0.344±0.027、0.593±0.017、0.899±0.030和2.920±0.045，PPAPDC1A蛋白的表达分别为0.514±0.030、1.185±0.096、1.379±0.084和1.761±0.072；子宫颈癌SiHa、HeLa、Caski和C33-A细胞中PPAPDC1A mRNA和蛋白的表达比较差异有统计学意义（P<0.05）。PPAPDC1A mRNA和蛋白在4种子宫颈癌细胞中表达的顺序为：C33-A细胞>Caski细胞>HeLa细胞>SiHa细胞。结论　PPAPDC1A基因在不同子宫颈癌细胞中存在差异表达。
Abstract:
Objective　To investigate the expression difference of phosphatidic acid phosphatase type 2 domain containing 1A （PPAPDC1A） in four kinds of human cervical carcinoma cells.Methods　Real-time quantitative polymerase chain reaction (qRT-PCR) and Western blot methods were used to detect the expressions of PPAPDC1A mRNA and protein in cervical carcinoma C33-A cells,Caski cells,HeLa cells and SiHa cells.Results　The expression of PPAPDC1A mRNA in SiHa cells，HeLa cells，Caski cells and C33-A cells was 0.344±0.027，0.593±0.017，0.899±0.030 and 2.920±0.045,respectively;the expression of PPAPDC1A protein in SiHa cells，HeLa cells，Caski cells and C33-A cells was 0.514±0.030，1.185±0.096，1.379±0.084 and 1.761±0.072,respectively.There were significant differences in the expressions of PPAPDC1A mRNA and protein in the four kinds of cervical carcinoma cells (P<0.05).The order of PPAPDC1A mRNA and protein expressions in the four kinds of cervical carcinoma cells was as follows:C33-A cells >Caski cells>HeLa cells>SiHa cells.Conclusion　PPAPDC1A gene is differentially expressed among C33-A cells,Caski cells,HeLa cells and SiHa cells.

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更新日期/Last Update: 2017-08-05