[1]徐亚沛,孙晓芳,朱方涛,等.肾康注射液在大鼠腹膜透析模型中对腹膜的保护作用[J].新乡医学院学报,2017,34(2):110-112.[doi:10.7683/xxyxyxb.2017.02.008]
　XU Ya-pei,SUN Xiao-fang,ZHU Fang-tao,et al.Protective effect of Shenkang injection on peritoneum in peritoneal dialysis rats[J].Journal of Xinxiang Medical University,2017,34(2):110-112.[doi:10.7683/xxyxyxb.2017.02.008]
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肾康注射液在大鼠腹膜透析模型中对腹膜的保护作用
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
34
期数:
2017年2
页码:
110-112
栏目:
基础研究
出版日期:
2017-02-05

文章信息/Info

Title:
Protective effect of Shenkang injection on peritoneum in peritoneal dialysis rats
作者:
徐亚沛1; 孙晓芳2; 朱方涛3; 任　蕾4; 邹　燕1; 崔东锋1; 吴宪鸣1
(1.郑州市第三人民医院肾内科，河南　郑州　450000；2.河南省人民医院检验科，河南　郑州　450000；3.郑州市第七人民医院心脏外科，河南　郑州　450000；4.郑州大学第一附属医院内分泌科，河南　郑州　450000)
Author(s):
XU Ya-pei1; SUN Xiao-fang2; ZHU Fang-tao3; REN Lei4; ZOU Yan1; CUI Dong-feng1; WU Xian-ming1
(1.Department of Nephrology，the Third People′s Hospital of Zhengzhou，Zhengzhou 450000，Henan Province，China；2.Department of Clinical Laboratory，Henan Provincial People′s Hospital，Zhengzhou 450000，Henan Province，China；3.Department of Cardiovascular Surgery，the Seventh People′s Hospital of Zhengzhou，Zhengzhou 450000，Henan Province，China；4.Department of Endocrinology，the First Affiliated Hospital of Zhengzhou University，Zhengzhou 450000，Henan Province，China)
关键词:
肾康注射液; 腹膜透析; 转化生长因子-β1; 纤维连接蛋白
Keywords:
Shenkang injection; peritoneal dialysis; transforming growth factor-β1; fibronectin
分类号:
R459.5
DOI:
10.7683/xxyxyxb.2017.02.008
文献标志码:
A
摘要:
目的　研究肾康注射液(SKI)对腹膜透析大鼠腹膜组织形态的影响，探讨其对腹膜保护的作用机制。方法　将45只健康Sprague-Dawley雄性大鼠随机分为对照组、模型组和SKI治疗组，每组15只。对照组大鼠给予腹腔注射生理盐水，模型组大鼠给予腹腔注射透析液建模，SKI治疗组大鼠在与模型组同样方法建模后腹腔注射肾康注射液治疗。3组大鼠均在腹腔注射后第8周进行超滤量测定，苏木精-伊红(HE)染色后观察3组大鼠壁层腹膜形态学变化，采用实时定量荧光聚合酶链式反应(RT-PCR)法测定3组大鼠腹膜转化生长因子-β1(TGF-β1)及纤维连接蛋白(FN)mRNA的表达。结果　模型组大鼠腹膜较对照组显著增厚，SKI治疗组大鼠壁层腹膜较模型组损伤较轻。与对照组比较，模型组大鼠腹膜超滤量显著下降(P<0.05)，腹膜TGF-β1 mRNA和FN mRNA表达量均显著升高(P<0.05)；与模型组比较，SKI治疗组大鼠腹膜超滤量升高(P<0.05)，腹膜TGF-β1 mRNA和FN mRNA表达量均显著下降(P<0.05)。结论　肾康注射液能够在一定程度上改善腹膜纤维化大鼠的腹膜功能，其机制可能是通过抑制腹膜组织中TGF-β1表达，减少FN积聚，从而减轻腹膜纤维化。
Abstract:
Objective　To investigate the effect of Shenkang injection(SKI) on the morphous of peritoneum in peritoneal dialysis rats and explore the potential protection mechanism of SKI on peritoneum.Methods　Forty-five health male Sprague-Dawley rats were randomly divided into control group，model group and SKI treatment group，with 15 rats in each group.The rats in control group were given normal saline by intra-peritoneal injection；the rats in model group were given peritoneal dialysis fluid by intra-peritoneal injection to establish peritoneal fibrotic model；the rats in SKI treatment was given SKI by intra-peritoneal injection after establishing the peritoneal fibrotic model.After eight weeks，ultrafiltration volume of all rats was detected to assess the function of peritoneal.Hematoxylin-eosin (HE) staining method was used to identify the morphology of visceral peritoneum tissue.The expressions of transforming growth factor-β1(TGF-β1) and fibronectin (FN) mRNA in peritoneum of rats were examined by real time polymerase chain reaction (RT-PCR).Results　Compared with control group，the thickness of peritoneal tissue of rats in model group increased significantly.Compared with model group，the thickness of peritoneal tissue of rats in SKI treatment group decreased obviously.The ultrafiltration volume of peritoneal of rats in model group was significantly lower than that in control group (P<0.05)，but the expressions of TGF-β1 and FN mRNA were significantly higher than those in the control group(P<0.05).The ultrafiltration volume of peritoneal of rats in SKI treatment group was significantly higher than that in model group (P<0.05)，but the expressions of TGF-β1 and FN mRNA were significantly lower than those in the model group(P<0.05).Conclusion　SKI can improve the peritoneal function of rats with peritoneal fibrosis through inhibitting the expression of TGF-β1 and decreasing the deposition of PN.

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更新日期/Last Update: 2017-02-05