[1]冯思佳,崔　静,杨　璐,等.集聚蛋白基因在不同宫颈癌细胞中的表达[J].新乡医学院学报,2016,33(5):365-368.[doi:10.7683/xxyxyxb.2016.05.006]
　FENG Si-jia,CUI Jing,YANG Lu,et al.Expression of Agrin gene in different human cervical carcinoma cells[J].Journal of Xinxiang Medical University,2016,33(5):365-368.[doi:10.7683/xxyxyxb.2016.05.006]
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集聚蛋白基因在不同宫颈癌细胞中的表达
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年5
页码:
365-368
栏目:
基础研究
出版日期:
2016-05-05

文章信息/Info

Title:
Expression of Agrin gene in different human cervical carcinoma cells
作者:
冯思佳; 崔　静; 杨　璐; 赵二趁; 柴　婕; 杨丹丹; 张翠翠; 王志慧; 郜培琼; 千新来
（新乡医学院基础医学院病理学教研室，河南　新乡　453003）
Author(s):
FENG Si-jia; CUI Jing; YANG Lu; ZHAO Er-chen; CHAI Jie; YANG Dan-dan; ZHANG Cui-cui; WANG Zhi-hui; GAO Pei-qiong; QIAN Xin-lai
（Department of Pathology，School of Basic Medical Sciences，Xinxiang Medical University，Xinxiang 453003，Henan Province，China）
关键词:
宫颈癌; 集聚蛋白; 信使RNA; 人乳头瘤病毒
Keywords:
cervical carcinoma; Agrin; messenger RNA; human papillomavirus
分类号:
R737.33
DOI:
10.7683/xxyxyxb.2016.05.006
文献标志码:
A
摘要:
目的　探讨集聚蛋白（Agrin）基因在人乳头瘤病毒（HPV）阳性和阴性宫颈癌细胞中表达的差异。方法　以人乳头瘤病毒16（HPV16）阳性的宫颈癌Caski细胞和SiHa细胞、HPV18阳性的宫颈癌HeLa细胞、HPV 阴性的宫颈癌C33-A细胞为研究对象。通过Western blot和实时荧光定量聚合酶链反应（PCR）等方法分别检测Agrin mRNA和蛋白在不同宫颈癌细胞中的表达。结果　实时荧光定量PCR和Western blot结果均显示，与HPV阴性的宫颈癌C33-A细胞比较，HPV16阳性的宫颈癌Caski和SiHa细胞、HPV18阳性的宫颈癌HeLa细胞中的Agrin mRNA和蛋白表达均显著增加（P<0.01或0.05），Agrin mRNA和蛋白在4种宫颈癌细胞中表达的顺序为：C33-A细胞<SiHa细胞<Caski细胞和HeLa细胞。结论　HPV阳性的宫颈癌细胞中Agrin基因的表达显著高于HPV 阴性的宫颈癌细胞，为深入研究Agrin基因与宫颈癌的关系及相关机制奠定了基础。
Abstract:
Objective　To investigate the expression of Agrin gene in human papillomavirus(HPV)-positive and-negative cervical carcinoma cells.Methods　Cervical carcinoma Caski cells(HPV16-positive)，SiHa cells(HPV16-positive)，HeLa cells(HPV18-positive) and C33-A cells(HPV-negative) were taken as the research objects.Real-time fluorescence quantitative polymerase chain reaction(PCR) and Western blot methods were used to detect the expressions of Agrin mRNA and protein in different cervical carcinoma cells.Results　Real-time fluorescence quantitative PCR and Western blot results indicated that compared with C33-A cells(HPV-negative)，the Agrin mRNA and protein expressions in Caski cells(HPV16-positive)，SiHa cells(HPV16-positive) and HeLa cells(HPV18-positive) were significantly increased(P<0.01 or 0.05).The order of Agrin mRNA and protein expressions in the four kinds of cervical carcinoma cells was as follows：C33-A cells<SiHa cells<Caski cells and HeLa cells.Conclusion　The expression of Agrin gene in HPV-positive cervical carcinoma cells is significantly higher than that in HPV-negative cervical carcinoma cells，which has laid a foundation for further research on the relationship between Agrin gene and cervical carcinoma and its mechanisms.

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更新日期/Last Update: 2016-05-05