[1]刘　云,马东红,崔　艳,等.黄芩苷对单侧输尿管梗阻模型大鼠肾脏氧化应激的保护作用[J].新乡医学院学报,2016,33(5):362-364.[doi:10.7683/xxyxyxb.2016.05.005]
　LIU Yun,MA Dong-hong,CUI Yan,et al.Protective effect of Baicalin on oxidative stress in rats with unilateral ureteral obstruction[J].Journal of Xinxiang Medical University,2016,33(5):362-364.[doi:10.7683/xxyxyxb.2016.05.005]
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黄芩苷对单侧输尿管梗阻模型大鼠肾脏氧化应激的保护作用
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年5
页码:
362-364
栏目:
基础研究
出版日期:
2016-05-05

文章信息/Info

Title:
Protective effect of Baicalin on oxidative stress in rats with unilateral ureteral obstruction
作者:
刘　云; 马东红; 崔　艳; 郭明好
（新乡医学院第一附属医院肾内科，河南　卫辉　453100）
Author(s):
LIU Yun; MA Dong-hong; CUI Yan; GUO Ming-hao
(Department of Nephrology，the First Affiliated Hospital of Xinxiang Medical University，Weihui 453100，Henan Province，China)
关键词:
输尿管梗阻; 氧化应激; 黄芩苷; 大鼠
Keywords:
unilateral ureteral obstruction; oxidative stress; Baicalin; rat
分类号:
R693
DOI:
10.7683/xxyxyxb.2016.05.005
文献标志码:
A
摘要:
目的　探讨黄芩苷对单侧输尿管梗阻（UUO）模型大鼠肾脏氧化应激的影响。方法　将90只大鼠随机分为假手术组、模型组、贝那普利组、黄芩苷组及黄芩苷+贝那普利组，每组18只。假手术组大鼠仅切开腹腔并游离左侧输尿管，但不结扎和离断，模型组和各干预组大鼠制备UUO模型。造模后贝那普利组大鼠给予贝那普利混悬液灌胃10 mg·kg-1·d-1，黄芩苷组大鼠给予黄芩苷溶液灌胃40 mg·kg-1·d-1，黄芩苷+贝那普利组大鼠给予黄芩苷溶液灌胃40 mg·kg-1·d-1 和贝那普利混悬液10 mg·kg-1·d-1，模型组与假手术组大鼠给予等量生理盐水灌胃。饲养14、21、28 d后处死各组大鼠，取肾脏标本，用可见光分光光度计法测定肾组织中超氧化物歧化酶（SOD）、总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-PX）的含量；酶联免疫吸附试验测定晚期氧化蛋白产物（AOPP）的含量。结果　与假手术组比较，模型组各时间点大鼠肾组织中SOD、GSH-PX活性及T-AOC均降低(P<0.05)，AOPP含量升高(P<0.05)。与模型组比较，黄芩苷组、贝那普利组和黄芩苷+贝那普利组各时间点大鼠肾组织中SOD、GSH-PX活性及T-AOC均显著升高(P<0.05)，AOPP含量降低(P<0.05)。黄芩苷组、贝那普利组和黄芩苷+贝那普利组3组之间各时间点大鼠肾组织中SOD、GSH-PX 活性、T-AOC及AOPP含量两两比较差异均无统计学意义(P>0.05)。结论　黄芩苷对UUO模型大鼠肾脏氧化应激具有保护作用。
Abstract:
Objective　To observe the protective effect of Baicalin on oxidative stress in rats with unilateral ureteral obstruction(UUO).Methods　Ninety Sprague-Dawley male rats were randomly divided into sham operation group，model group，benazepril group,Baicalin group，Baicalin+benazepril group，with 18 rats in each group.The UUO model was established in the model group and the intervention group rats.In sham operation group，the rats underwent abdominal incision and free left ureter，but not ligation and amputation.After modeling，the rats in benazepril group received intragastric administration of benazepril suspension 10 mg·kg-1·d-1;the rats in Baicalin group received intragastric administration of Baicalin 40 mg·kg-1·d-1;the rats in Baicalin+benazepril group received intragastric administration of Baicalin 40 mg·kg-1·d-1 and benazepril suspension 10 mg·kg-1·d-1;the rats in model group and sham operation were given the same volume of normal saline.Six rats were sacrificed in each group on the 14th，21th，28th day after experiment onset and the kidney were obtained.The visible range spectrophotometer method was used to determine the activity of superoxide dismutase(SOD) and glutathion peroxidase(GSH-PX)，and the total antioxidant capacity(T-AOC)in kidney tissue.The enzyme linked immunosorbent assay was used to determine the contents of advanced oxidation protein products(AOPP).Results　Compared with the sham operation group，the activity of SOD and GSH-PX，and the T-AOC level in model group decreased(P<0.05) and the contents of AOPP increased(P<0.05) in each time point.Compared with the model group，the activity of SOD and GSH-PX，and the T-AOC level in Baicalin group，benazepril group and Baicalin+benazepril group increased(P<0.05)and the contents of AOPP decreased(P<0.05) in each time point.There was no statistic difference of the activity of SOD and GSH-PX，and the level of T-AOC and AOPP among the Baicalin group，benazepril group and Baicalin+benazepril group(P>0.05).Conclusion　Baicalin has the protective effects on the oxidative stress in rats with UUO.

参考文献/References:

［1］　张磊，徐浩，何勇，等.黄芩苷对大鼠心室肌细胞动作电位的影响［J］.新乡医学院学报，2015，32(1):28-31.
［2］　INOUE T，JACKSON E K.Strong antiproliferative effects of baicaleinin cultured rat hepatic stellate cells［J］.Eur J Pharmacol，1999，378（1）：129-135.
［3］　刘威，陈晓玲.黄芩苷对博莱霉素诱导的肺纤维化的影响［J］.中国应用生理学杂志，2009，25（2）：145-148.
［4］　刘希杰，冯文玉，耿磊，等.黄芩苷通过抑制核因子-κB表达减轻胆管不全梗阻大鼠肠黏膜损伤［J］.中华实用儿科临床杂志，2014，29(7):514-517.
［5］　BALIGA R，UEDA N，WALKER P D.Oxidant mechanisms in toxic acute renal failure［J］.Drug Metab Rev，1999，31（4）：971-997.
［6］　KLAHR S，MORRISSEY J J.Obstructive nephropathy and renal fibrosis［J］.Am J Physiol Renal Physiol，2002，283(5):F861-F875.
［7］　张晓暄，李银杰，郑伟，等.单侧输尿管梗阻大鼠肾间质纤维化中氧化应激的变化［J］.中国现代医学杂志，2007，17（4）：414-417.
［8］　王瑞良，张洪梅，苏青.苯那普利对糖尿病大鼠组织氧化应激和羧基应激的影响［J］.中国医师杂志，2007，11（11）：1475-1477.
［9］　RICARDO S D，DING G，EUFEMIO M，et al.Antioxidant expression in experimental hydronephrosis:role of mechanical stretch and growth factors［J］.Am J Physiol Renal Physiol，1997，272(6 Pt 2):F789-F798.
［10］　WITKO-SARSAT V，GAUSSON V，NGUYEN A T，et al.AOPP-induced activation of human neutrophil and monocyte oxidative metabolism:a potential target for N-acetylcysteine treatment in dialysis patients［J］.Kidney Int，2003，64(1):82-91.
［11］　SMALL D M，COOMBES J S，BENNETT N，et al.Oxidative stress，anti-oxidant therapies and chronic kidney disease［J］.Nephrology(Carlton)，2012，17(4):311-321.
［12］　RENKE M，TYLICKI L，KNAP N，et al.High-dose angiotensin-converting enzyme inhibitor attenuates oxidative stress in patients with chronic kidney disease［J］.Nephrol Dial Transplant，2009，24(2):689-690.
［13］　SWAMINATHAN S，SHAH S V.Novel approaches targeted toward oxidative stress for the treatment of chronic kidney disease［J］.Curr Opin Nephrol Hypertens，2008，17(2):143-148.
［14］　王超云，傅风华，田京伟，等.黄芩苷对化学性肝损伤的保护作用［J］.中草药，2005，36(5)：730-732.
［15］　谢红东，杨珂，穆焕德，等.黄芩提取物对大鼠肾间质纤维化的作用及其抗氧化机制［J］.中国中西医结合肾病杂志，2009，10（3）：240-242.
［16］　赵曙光，李慧艳，赵保民，等.黄芩甙对重症急性胰腺炎大鼠胰腺氧化应激的保护作用［J］.胃肠病学和肝病学杂志，2009，18（9）：863-865.

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更新日期/Last Update: 2016-05-05