[1]王坤,朱海松,李军.血浆微RNA-191、微RNA-23a和微RNA-145表达水平与前列腺癌患者预后的相关性[J].新乡医学院学报,2023,40(12):1140-1145.[doi:10.7683/xxyxyxb.2023.12.008]
　WANG Kun,ZHU Haisong,LI Jun.Correlation between the expression levels of plasma microRNA-191,microRNA-23a and microRNA-145 and the prognosis of patients with prostate cancer[J].Journal of Xinxiang Medical University,2023,40(12):1140-1145.[doi:10.7683/xxyxyxb.2023.12.008]
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血浆微RNA-191、微RNA-23a和微RNA-145表达水平与前列腺癌患者预后的相关性
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
40卷
期数:
2023年12
页码:
1140-1145
栏目:
临床研究
出版日期:
2023-12-05

文章信息/Info

Title:
Correlation between the expression levels of plasma microRNA-191,microRNA-23a and microRNA-145 and the prognosis of patients with prostate cancer
作者:
王坤; 朱海松; 李军
(驻马店市中心医院泌尿外一科，河南　驻马店　463000)
Author(s):
WANG Kun; ZHU Haisong; LI Jun
(No.1 Department of Urology,Zhumadian Central Hospital,Zhumadian 463000,Henan Province,China)
关键词:
前列腺癌; 微RNA-191; 微RNA-23a; 微RNA-145
Keywords:
prostate cancer; microRNA-191; microRNA-23a; microRNA-145
分类号:
R735.25
DOI:
10.7683/xxyxyxb.2023.12.008
文献标志码:
A
摘要:
目的　探讨前列腺癌患者血浆微RNA（miR）-191、miR-23a和miR-145表达水平及其与预后的相关性。
方法　选择2019年12月至2021年12月驻马店市中心医院收治的60例前列腺癌患者为观察组，另选择同期健康体检者60例为对照组。采用实时荧光定量聚合酶链式反应法检测2组受试者的血浆miR-191、miR-23a和miR-145表达水平。根据预后将观察组患者分为预后不良组（n=11）和预后良好组（n=49）。比较观察组患者治疗前后血浆miR-191、miR-23a和miR-145表达水平，比较预后不良组和预后良好组患者的一般资料，绘制受试者操作特征曲线分析血浆miR-191、miR-23a和miR-145表达对患者预后的预测价值。
结果　观察组患者血浆miR-191、miR-23a相对表达量显著高于对照组，miR-145相对表达量显著低于对照组（P<0.05）。观察组患者治疗后血浆miR-191、miR-23a相对表达量显著低于治疗前，miR-145相对表达量显著高于治疗前（P<0.05）。预后不良组与预后良好组患者的年龄、体质量指数、吸烟史、饮酒史、前列腺体积比较差异无统计学意义（P>0.05）；2组患者的TNM分期、Gleason评分、血清前列腺特异抗原水平及血浆miR-191、miR-23a、miR-145相对表达量比较差异有统计学意义（P<0.05）。血浆miR-191、miR-23a和miR-145水平是前列腺癌患者预后的影响因素（P<0.05）。血浆miR-191、miR-23a、miR-145均对前列腺癌患者预后有预测价值，三者联合预测前列腺癌患者预后的价值显著高于血浆miR-191、miR-23a、miR-145单独预测的价值（P<0.05）。
结论　前列腺癌患者血浆中miR-191、miR-23a表达升高， miR-145表达降低。血浆miR-191、miR-23a、miR-145联合检测对前列腺癌患者的预后具有较高的预测价值。
Abstract:
Objective　To investigate the expression levels of microRNA (miR)-191,miR-23a,and miR-145 in plasma of patients with prostate cancer and their correlation with prognosis.
Methods　Sixty prostate cancer patients admitted to Zhumadian Central Hospital from December 2019 to December 2021 were selected as the observation group,and 60 healthy subjects who underwent physical examinations during the same period were selected as the control group.The expression levels of miR-191,miR-23a and miR-145 in plasma of subjects in the two groups were measured by real-time fluorescence quantitative polymerase chain reaction.The patients in the observation group were divided into poor prognosis group (n=11) and good prognosis group (n=49) according to their prognosis.The expression levels of miR-191,miR-23a,and miR-145 in plasma of prostate cancer patients were compared before and after treatment.The general data of patients was compared between the poor prognosis group and the good prognosis group.The receiver operator characteristic curve was drawed to analyse the predictive value of plasma miR-191,miR-23a,and miR-145 levels on prognosis.
Results　The relative expression levels of miR-191 and miR-23a in plasma of patients in the observation group were significantly higher than those in the control group,while the relative expression level of miR-145 was significantly lower than that in the control group (P<0.05).The relative expression levels of miR-191 and miR-23a of patients in the observation after treatment were significantly lower than those before treatment,while the relative expression level of miR-145 was significantly higher than that before treatment (P<0.05).There was no significant difference in age,body mass index,smoking history,alcohol consumption history,and prostate volume of patients between the poor prognosis group and the good prognosis group(P>0.05);there was significant differences in TNM staging,Gleason score,serum prostate specific antigen level and relative expressions of miR-191,miR-23a,miR-145 in plasma of patients between the two groups (P<0.05).Plasma miR-191,miR-23a and miR-145 levels were influence factor for prognosis in patients with prostate cancer (P<0.05).There was predictive value of plasma miR-191,miR-23a and miR-145 for prognosis of prestate cancer patients;the combined predictive value of plasma miR-191,miR-23a and miR-145 for the prognosis of prostate cancer patients was significantly higher than that of plasma miR-191,miR-23a and miR-145 alone for the prognosis of prostate cancer patients (P<0.05).
Conclusion　The expression of miR-191 and miR-23a in plasma increases and the expression of miR-145 in plasma decreases in patients with prostate cancer.The combined detection of plasma miR-191,miR-23a and miR-145 has higher predictive value for the prognosis of prostate cancer.

参考文献/References:

［1］　BAKIR W A I,GAIDAN H A,AL-KAABI M M.Immunohistochemical expression of interlukin10 (IL10) and heat shock protein-90 (HSP-90) in prostatic carcinoma［J］.Indian J Pathol Microbiol,2020,63(2):230-234.
［2］　朱海松.经腹膜外与经腹腔腹腔镜前列腺癌根治术治疗前列腺癌疗效比较［J］.新乡医学院学报,2022,39(7):626-630.
ZHU H S.Comparison of curative effect between extraperitoneal and transabdominal laparoscopic radical prostatectomy for prostate cancer［J］.J Xinxiang Med Univ,2022,39(7):626-630.
［3］　侯国栋,朱峰,张会清，等.阿比特龙联合多西他赛和泼尼松治疗转移性去势抵抗性前列腺癌的临床疗效及安全性［J］.新乡医学院学报,2023,40(7):634-638.
HOU G D,ZHU F,ZHANG H Q,et al.Curative effect and safety of abiraterone combined with docetaxel and prednisone in the treatment of metastatic castration-resistant prostate cancer［J］.J Xin-xiang Med Univ,2023,40(7):634-638.
［4］　ZHANG Z.MiR-124-3p suppresses prostatic carcinoma by targeting PTGS2 through the AKT/NF-κB pathway［J］.Mol Biotechnol,2021,63(7):621-630.
［5］　聂永莉,汤世敏,彭芳,等.miRNA-191对前列腺癌的增殖、迁移侵袭能力的影响及其机制［J］.中国应用生理学杂志,2020,36(6):637-641.
NIE Y L,TANG S M,PENG F,et al.miRNA-191 promotes prolife-ration,migration and invasion of prostate cancer by targeting PLCD1［J］.Chin J Appl Physiol,2020,36(6):637-641.
［6］　蔡松旺,黄怀球,李小娟,等.微小RNA-23a调节前列腺癌细胞骨架蛋白的分子机制［J］.中华实验外科杂志,2012,29(8):1572-1573.
CAI S W,HUANG H Q,LI X J,et al.Mechanism of miR-23a on the regulation of the cytoskeleton proteins of prostate cancer cells［J］.Chin J Exp Surg,2012,29(8):1572-1573.
［7］　XIE X,DAI J,HUANG X,et al.MicroRNA-145 inhibits proliferation and induces apoptosis in human prostate carcinoma by upregulating long non-coding RNA GAS5［J］.Oncol Lett,2019,18(2):1043-1048.
［8］　ICZKOWSKI K A,VAN LEENDERS G J L H.Eight endorsements of the international society of urological pathology from the 2019 consensus conference on grading of prostatic carcinoma［J］.J Urol,2021,205(1):8-10.
［9］　刘硕,王硕,李慧超,等.2000—2017年北京市前列腺癌发病和死亡流行趋势及发病年龄变化特征分析［J］.中国肿瘤,2021,30(7):495-505.
LIU S,WANG S,LI H C,et al.Analysis on the trends of incidence,mortality and characteristics of age of onset for prostate cancer in Beijing,2000—2017［J］.China Cancer,2021,30(7):495-505.
［10］　OLORUNSOLA I S,ETONYEAKU A C,LEKWA B O,et al.Bilateral secondary testicular,epididymal and spermatic cords carcinoma of prostatic origin:a case report and review of theliterature［J］.J Med Case Rep,2021,15(1):222.
［11］　赵洁,王红,薛力.血清PSA水平下降速率联合改良PI-RADS评分在鉴别前列腺良性疾病与前列腺癌中的价值［J］.现代泌尿外科杂志,2020,25(1):32-35.
ZHAO J,WANG H,XUE L.The diagnostic value of PSA decline rate combined with modified PI-RADS score in the identification of benign prostatic diseases and prostate cancer［J］.J Mod Urol,2020,25(1):32-35.
［12］　NATH C K,BARMAN B,PHUKAN P,et al.Prostate-specific antigen density:a measurement to differentiate benign hypertrophy of prostate from prostate carcinoma［J］.J Lab Physicians,2020,12(1):44-48.
［13］　MITCHELL P S,PARKIN R K,KROH E M,et al.Circulating microRNAs as stable blood-based markers for cancer detection［J］.Proc Natl Acad Sci U S A,2008,105(30):10513-10518.
［14］　李飞,黄毅,王丽婷,等.MiR-191过表达抑制NK细胞的肿瘤杀伤活性和细胞凋亡［J］.免疫学杂志,2020,36(10):853-858.
LI F,HUANG Y,WANG L T,et al.Overexpression of miR-191 inhibits the tumor-killing activity and apoptosis of natural killer cells in mice［J］.Immunol J,2020,36(10):853-858.
［15］　TIAN X,ZHANG Z.MiR-191/DAB2 axis regulates the tumorigenicity of estrogen receptor-positive breast cancer［J］.IUBMB Life,2018,70(1):71-80.
［16］　陈红英,高贵峰,闫少茹,等.MiR-191靶向调控SATB1对肝癌细胞增殖和侵袭的影响［J］.山东医药,2022,62(1):39-43.
CHEN H Y,GAO G F,YAN S R,et al.Effects of miR-191 regulating SATB1 expression on proliferation and invasion of hepatoma cells［J］.Shandong Med J,2022,62(1):39-43.
［17］　张冲,杨鹏,刘振湘,等.前列腺癌细胞miR-191-5p表达变化及其对细胞增殖和凋亡的影响［J］.山东医药,2018,58(8):51-53.
ZHANG C,YANG P,LIU Z X,et al.Changes of miR-191-5p expression in prostate cancer cells and its effect on cell proliferation and apoptosis［J］.Shandong Med J,2018,58(8):51-53.
［18］　白璐,张森,卢亚乐,等.前列腺癌发病机制中信号通路的研究进展［J］.中国医学工程,2020,28(9):39-43.
BAI L,ZHANG S,LU Y L,et al.Advances in research on signaling pathways in pathogenesis of prostate cancer［J］.China Med Eng,2020,28(9):39-43.
［19］　林楠,许子琪,蔡雁,等.MiR-23a在宫颈癌患者组织血清中的表达及相关性分析［J］.现代生物医学进展,2021,21(8):1507-1510.
LIN N,XU Z Q,CAI Y,et al.Expression and correlation analysis of miR-23a in serum of patients with cervical cancer［J］.Prog Mod Biomed,2021,21(8):1507-1510.
［20］　LIU J,FAN L,YU H,et al.Endoplasmic reticulum stress causes liver cancer cells to release exosomal miR-23a-3p and up-regulate programmed death ligand 1 expression in macrophages［J］.Hepatology,2019,70(1):241-258.
［21］　高永涛,白浪,樊华,等.MiR-23a/SATB2信号通路对结直肠癌SW480细胞凋亡和转移的影响［J］.国际消化病杂志,2021,41(4):289-295.
GAO Y T,BAI L,FAN H,et al.Effect of miR-23a/SATB2 signaling pathway on apoptosis and metastasis of colorectal cancer SW480 cells［J］.Int J Dig Dis,2021,41(4):289-295.
［22］　陈建清,胡良凯,徐选福.血清miR-31和miR-23a表达水平在胃腺癌检测中的临床意义［J］.河北医药,2019,41(13):1945-1948.
CHEN J Q,HU L K,XU X F.Clinical significance of serum levels of miR-31 and miR-23a in detection of gastric adenocarcinoma［J］.Hebei Med J,2019,41(13):1945-1948.
［23］　胡鑫,朱娜,王琴.NSCLC患者血清miR-23a和miR-29a水平表达对靶向治疗效果的预测价值［J］.现代检验医学杂志,2022,37(2):61-65.
HU X,ZHU N,WANG Q.Predictive value of serum miR-23a and miR-29a levels in patients with NSCLC for targeted therapy［J］.J Mod Lab Med,2022,37(2):61-65.
［24］　刘亚杰,马晓波.MiR-23a和MTSS1在弥漫大B细胞淋巴瘤中的表达及其临床意义［J］.川北医学院学报,2020,35(3):401-404.
LIU Y J,MA X B.Expression and clinical significance of miR-23a and MTSS1 in diffuse large B-cell lymphoma［J］.J North Sichuan Med Coll,2020,35(3):401-404.
［25］　蔡松旺,李小娟,陈锐涵,等.微小RNA-23a-p21活化激酶6通路对前列腺癌细胞迁移与侵袭能力的影响［J］.中华实验外科杂志,2012,29(7):1360-1362.
CAI S W,LI X J,CHEN R H,et al.Impact of miRNA-23a-p21-activated kinase 6 signaling on the migration and invasion activities of prostate cancer cells［J］.Chin J Exp Surg,2012,29(7):1360-1362.
［26］　ZHU Y,WANG C,BECKER S A,et al.miR-145 antagonizes SNAI1-mediated stemness and radiation resistance in colorectal cancer［J］.Mol Ther,2018,26(3):744-754.
［27］　郑海艺,王国栋,张元民.MiR-145传导通路与肿瘤的研究进展［J］.中国临床实用医学,2019,10(5):74-77.
ZHENG H Y,WANG G D,ZHANG Y M.Research progress of miR-145 conduction pathway and tumor［J］.China Clin Pract Med,2019,10(5):74-77.
［28］　张小鹰,何金花,王浩,等.羽扇豆醇联合miR-145-5p对前列腺癌细胞增殖与凋亡的影响［J］.中国病理生理杂志,2020,36(8):1413-1421.
ZHANG X Y,HE J H,WANG H,et al.Effects of lupeol and miR-145-5p on proliferation and apoptosis of prostate carcinoma cells［J］.Chin J Pathophysiol,2020,36(8):1413-1421.
［29］　赵成桂,徐鸿波,许斌.MiR-143/miR-145簇启动子区rs4705342基因突变与汉族人群前列腺癌发病的相关性研究［J］.中华男科学杂志,2019,25(8):696-702.
ZHAO C G,XU H B,XU B.The rs4705342 gene mutation in the promoter region of the miR-143/145 cluster associated with the risk of prostate cancer in the Chinese Han population［J］.Natl J Androl,2019,25(8):696-702.

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[2]牛保华,高新平,胡艳牧.p16 蛋白在前列腺癌的表达及其意义[J].新乡医学院学报,2001,18(04):251.
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更新日期/Last Update: 2023-12-05