[1]裴晓东,孙占勇,陈世军.黄芩素对乳腺癌MDA-MB-231细胞侵袭转移的影响及其相关机制[J].新乡医学院学报,2021,38(5):406-412.[doi:10.7683/xxyxyxb.2021.05.002]
　PEI Xiaodong,SUN Zhanyong,CHEN Shijun.Effect of baicalein on invasion and metastasis of breast cancer cells MDA-MB-231 and its relative mechanism[J].Journal of Xinxiang Medical University,2021,38(5):406-412.[doi:10.7683/xxyxyxb.2021.05.002]
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黄芩素对乳腺癌MDA-MB-231细胞侵袭转移的影响及其相关机制
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
38
期数:
2021年5
页码:
406-412
栏目:
基础研究
出版日期:
2021-05-05

文章信息/Info

Title:
Effect of baicalein on invasion and metastasis of breast cancer cells MDA-MB-231 and its relative mechanism
作者:
裴晓东; 孙占勇; 陈世军
（郑州市第一人民医院普外科，河南　郑州　450000）
Author(s):
PEI Xiaodong; SUN Zhanyong; CHEN Shijun
(Department of General Surgery,Zhengzhou First People′s Hospital,Zhengzhou 450000,Henan Province,China)
关键词:
黄芩素; 乳腺癌; 侵袭; 转移; 转移相关肺腺癌转录本1
Keywords:
baicaleinbreast cancerinvasionmetastasismetastasis-associated lung adenocarcinoma transcript 1
分类号:
R730.53
DOI:
10.7683/xxyxyxb.2021.05.002
文献标志码:
A
摘要:
目的　探讨黄芩素对人乳腺癌MDA-MB-231细胞侵袭、转移的影响及相关分子机制。方法　将对数生长期MDA-MB-231细胞随机分为对照组和20、40、80、160 μmol·L-1黄芩素处理组，分别给予0、20、40、80、160 μmol·L-1黄芩素干预；采用划痕愈合实验检测各组MDA-MB-231细胞的转移能力，Transwell小室实验检测各组MDA-MB-231细胞的侵袭能力，蛋白质印迹法检测各组细胞中上皮型钙黏蛋白(CDH1)、蜗牛家族转录抑制因子（SNAIL）、波形蛋白（vimentin）的相对表达量，实时荧光定量聚合酶链式反应检测各组MDA-MB-231细胞中转移相关肺腺癌转录本1（MALAT1）mRNA相对表达量。采用聚合酶链式反应及pcDNA3.1载体构建MALAT1过表达载体，转染至对数生长期 MDA-MB-231细胞，将pcDNA3.1-MALAT1过表达MDA-MB-231细胞随机分为对照组和20、40、80、160 μmol·L-1黄芩素处理组，分别给予0、20、40、80、160 μmol·L-1黄芩素干预，使用蛋白质印迹法检测各组细胞中CDH1、SNAIL、vimentin蛋白相对表达量。结果　20、40 μmol·L-1黄芩素处理组与对照组细胞转移率、侵袭率比较差异无统计学意义（P>0.05）；80、160 μmol·L-1黄芩素处理组细胞转移率、侵袭率低于对照组（P<0.05）。80、160 μmol·l-1黄芩素处理组细胞转移率低于20 μmol·L-1黄芩素处理组（P<0.05），160 μmol·l-1黄芩素处理组细胞转移率低于80 μmol·L-1黄芩素处理组（P<0.05）；其余各剂量组之间细胞转移率比较差异无统计学意义（>P>0.05）。160 μmol·L-1黄芩素处理组细胞侵袭率低于20、40 μmol·L-1黄芩素处理组（P<0.05）；其余各剂量黄芩素处理组之间细胞侵袭率比较差异无统计学意义（>P>0.05）。20 μmol·L-1黄芩素处理组细胞中CDH1、vimentin和SNAIL蛋白相对表达量与对照组比较差异均无统计学意义（P>0.05）；40 μmol·L-1黄芩素处理组细胞中SNAIL蛋白相对表达量低于对照组（P<0.05），cdh1和vimentin蛋白相对表达量与对照组比较差异无统计学意义（>P>0.05）；80、160 μmol·L-1黄芩素处理组细胞中CDH1蛋白相对表达量高于对照组和20 μmol·L-1黄芩素处理组（P<0.05），vimentin和snail蛋白相对表达量低于对照组和20 μmol·l-1黄芩素处理组（P<0.05）；160 μmol·l-1黄芩素处理组细胞中CDH1蛋白相对表达量高于40 μmol·L-1黄芩素处理组（P<0.05），vimentin和snail蛋白相对表达量低于40 μmol·l-1黄芩素处理组（P<0.05）；其余各剂量黄芩素处理组细胞中cdh1、vimentin和snail蛋白相对表达量比较差异均无统计学意义（>P>0.05）。20、40、80、160 μmol·L-1黄芩素处理组细胞中MALAT1 mRNA相对表达量低于对照组（P<0.05）；80、160 μmol·l-1黄芩素处理组细胞中MALAT1 mRNA相对表达量低于20 μmol·L-1黄芩素处理组（P<0.05），160 μmol·l-1黄芩素处理组细胞中MALAT1 mRNA相对表达量低于40 μmol·L-1黄芩素处理组（P<0.05）；其余各剂量黄芩素处理组细胞中malat1 mrna相对表达量比较差异无统计学意义（P>0.05）。pcDNA3.1-MALAT1过表达MDA-MB-231细胞中MALAT1相对表达量高于空载体MDA-MB-23细胞（P<0.05）。20、40、80、160 μmol·l-1黄芩素处理组pcDNA3.1-MALAT1过表达MDA-MB-231细胞中CDH1、vimentin和SNAIL蛋白相对表达量与对照组比较差异无统计学意义（P>0.05）。结论　黄芩素可通过抑制MALAT1的表达而抑制MDA-MB-231细胞的上皮-间质转化过程，进而抑制细胞侵袭和转移能力。undefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefined/i="">/sup="">/sup="">/sup="">/html>
Abstract:
Objective　To investigate the effect and molecular mechanism of baicalein on invasion and metastasis of breast cancer cells MDA-MB-231.Methods　　The MDA-MB-231 cells in logarithmic phase were randomly divided into control group and 20,40,80,160 μmol·L-1 baicalein treatment groups,the cells in the five groups were treated with 0,20,40,80,160 μmol·L-1 baicalein.Wound-healing assay was used to detect the migration ability of MDA-MB-231 cells in each group;Transwell assay was used to detect the invasion ability of MDA-MB-231 cells in each group;Western blot was used to detect the relative expression of cadherin 1(CDH1),Snail family transcriptional repressor(SNAIL) and vimentin protein of MDA-MB-231 cells in each group;real-time quantitative polymerase chain reaction was used to detect the relative expression of metastasis-associated lung adenocarcinoma transcript 1(MALAT1) of MDA-MB-231 cells in each group.The overexpression vector of MALAT1 was constructed through polymerase chain reaction and inserted into pcDNA3.1 vector.MDA-MB-231 cells in logarithmic phase were transfected with pcDNA3.1-MALAT1 overexpression plasmid,and then were randomly divided into control group and 20,40,80,160 μmol·L-1 baicalein treatment groups;the cells in the five groups were treated with 0,20,40,160 μmol·L-1 baicalein.Western blot was used to detect the relative expression of CDH1,SNAIL and vimentin protein of the cells in each group.Results　There was no significant difference in the migration rate and invasion rate of cells between the 20,40 μmol·L-1 baicalein treatment groups and control group(P>0.05).The migration rate and invasion rate of cells in the 80,160 μmol·L-1 baicalein treatment groups were significantly lower than those in the control group (P<0.05).The migration rate of cells in the 80,160 μmol·L-1 baicalein treatment groups was significantly lower than that in the 20 μmol·L-1 baicalein treatment group (P<0.05).The migration rate of cells in the 160 μmol·L-1 baicalein treatment group was significantly lower that in the 80 μmol·L-1 baicalein treatment group (P<0.05).There was no significant difference in the migration rate among the other different concentration of baicalein treatment groups (P>0.05).The invasion rate of cells in the 160 μmol·L-1 baicalein treatment group was significantly lower than that in the 20,40 μmol·L-1 baicalein treatment groups (P<0.05).There was no significant difference in the invasion rate among the other different concentration of baicalein treatment groups (P>0.05).There was no significant difference in the relative expressions of CDH1,vimentin and SNAIL protein between the 20 μmol·L-1 baicalein treatment group and control group (P>0.05).The relative expressions of SNAIL protein in the 40 μmol·L-1 baicalein treatment group was significantly lower than that in the control group (P<0.05);there was no significant difference in the relative expressions of CDH1 and vimentin protein between the 40 μmol·L-1 baicalein treatment group and control group (P>0.05).The relative expression of CDH1 protein in the 80,160 μmol·L-1 baicalein treatment groups was significantly higher than that in the control group and 20 μmol·L-1 baicalein treatment group (P<0.05);the relative expressions of vimentin and SNAIL protein in the 80,160 μmol·L-1 baicalein treatment groups were significantly lower than those in the control group and 20 μmol·L-1 baicalein treatment group (P<0.05).The relative expression of CDH1 protein in the 160 μmol·L-1 baicalein treatment group was significantly higher than that in the 40 μmol·L-1 baicalein treatment group (P<0.05);the relative expressions of vimentin and SNAIL protein in the 160 μmol·L-1 baicalein treatment group were significantly lower than those in the 40 μmol·L-1 baicalein treatment group (P<0.05).There was no significant difference in the relative expressions of CDH1,SNAIL and vimentin protein among the other different concentration of baicalein treatment groups (P>0.05).The relative expression of MALAT1 mRNA in 20,40,80,160 μmol·L-1 baicalein treatment groups was significantly lower than that in the control group (P<0.05).The relative expression of MALAT1 mRNA in the 80,160 μmol·L-1 baicalein treatment groups was significantly lower than that in the 20 μmol·L-1 baicalein treatment group (P<0.05).The relative expression of MALAT1 mRNA in the 160 μmol·L-1 baicalein treatment group was significantly lower than that in the 40 μmol·L-1 baicalein treatment group (P<0.05).The relative expression of MALAT1 mRNA in MDA-MB-231 cells transfected with pcDNA3.1-MALAT1 was higher than that in MDA-MB-231 cells transfected with empty vector (P<0.05).There was no significant difference in the relative expression of MALAT1 mRNA among the other different concentration of baicalein treatment groups (P>0.05).There was no significant difference in the relative expressions of CDH1,SNAIL and vimentin protein in MDA-MB-231 cells transfected with pcDNA3.1-MALAT1 overexpression plasmid between the 20,40,80,160 μmol·L-1 baicalein treatment groups and control group(P>0.05).Conclusion　Baicalin can inhibit the epithelial-mesenchymal transition process by inhibiting the expression of MALAT1,thereby inhibiting the invasion and migration of MDA-MB-231 cells.

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更新日期/Last Update: 2021-05-05