[1]王　静,康媛洁,王　贞,等.DEAH盒解旋酶15对转化生长因子-β1诱导的呼吸道平滑肌细胞增殖和迁移的影响[J].新乡医学院学报,2021,38(5):413-417.[doi:10.7683/xxyxyxb.2021.05.003]
　WANG Jing,KANG Yuanjie,WANG Zhen,et al.Effect of DEAH-box helicase 15 on the proliferation and migration of airway smooth muscle cells induced by transforming growth factor-β1[J].Journal of Xinxiang Medical University,2021,38(5):413-417.[doi:10.7683/xxyxyxb.2021.05.003]
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DEAH盒解旋酶15对转化生长因子-β1诱导的呼吸道平滑肌细胞增殖和迁移的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
38
期数:
2021年5
页码:
413-417
栏目:
基础研究
出版日期:
2021-05-05

文章信息/Info

Title:
Effect of DEAH-box helicase 15 on the proliferation and migration of airway smooth muscle cells induced by transforming growth factor-β1
作者:
王　静1; 康媛洁2; 王　贞2; 唐　青2; 陈　瑶2
（1.西安市儿童医院呼吸哮喘中心，陕西　西安　710003； 2.西安市儿童医院呼吸二科，陕西　西安　710003）
Author(s):
WANG Jing1; KANG Yuanjie2; WANG Zhen2; TANG Qing2; CHEN Yao2
(1.Respiratory Asthma Center，Xi′an Children′s Hospital，Xi′an 710003，Shaanxi Province,China2.Department of Respiratory Medicine，Xi′an Children′s Hospital，Xi′an 710003，Shaanxi Province,China)
关键词:
DEAH盒解旋酶15; 核因子-κB信号通路; 丝裂原活化蛋白激酶信号通路; 哮喘; 增殖; 迁移
Keywords:
DEAH-box helicase 15; nuclear factor-κB signaling pathway; mitogen-activated protein kinase signaling pathway; asthma; proliferation; migration
分类号:
R563
DOI:
10.7683/xxyxyxb.2021.05.003
文献标志码:
A
摘要:
目的　探讨DEAH盒解旋酶15（DHX15）对转化生长因子-β1（TGF-β1）诱导的呼吸道平滑肌细胞（ASMCs）增殖和迁移的影响。方法　将TGF-β1（20 mg·L-1）诱导的ASMCs随机分为对照组、阴性对照组和沉默组。阴性对照组和沉默组细胞分别转染control-siRNA和DHX15-siRNA质粒，对照组细胞不作处理。 转染24 h后，采用实时荧光定量聚合酶链反应法检测各组细胞中DHX15 mRNA相对表达量，细胞计数试剂盒-8实验检测各组细胞增殖能力，Transwell小室实验检测各组细胞迁移能力，Western blot法检测各组细胞中磷酸化p65（P-p65）、磷酸化核因子抑制蛋白（P-IκB）、磷酸化c-Jun氨基末端激酶（P-JNK）、磷酸化p38（P-p38）蛋白的表达。 结果　对照组和阴性对照组ASMCs中DHX15 mRNA相对表达量、细胞增殖能力、细胞迁移能力比较差异无统计学意义（P>0.05）；沉默组ASMCs中DHX15 mRNA相对表达量、细胞增殖能力、细胞迁移能力显著低于对照组和阴性对照组（P<0.05）。对照组和阴性对照组asmcs中p-p65、p-iκb、p-jnk和p-p38蛋白相对表达量比较差异无统计学意义（>P>0.05）；沉默组ASMCs中p-p65、p-IκB、p-JNK和p-p38蛋白相对表达量均显著低于对照组和阴性对照组（P<0.05）。>结论　干扰DHX15表达可显著抑制TGF-β1诱导的ASMCs的增殖和迁移能力，其机制可能与抑制核因子-κB和丝裂原活化蛋白激酶信号通路有关。undefinedundefined
Abstract:
Objective　To investigate the effect of DEAH-box helicase 15 (DHX15) on the proliferation and migration of airway smooth muscle cells (ASMCs) induced by transforming growth factor-β1 (TGF-β1).Methods　　The ASMCs induced by TGF-β1 (20 mg·L-1) were randomly divided into control group，negative control group and silent group.The cells in the negative control group and the silence group were respectively transfected with control-siRNA and DHX15-siRNA plasmids，and the cells in the control group were not treated.After 24 hours of transfection，the relative expression level of DHX15 mRNA of the cells in each group was detected by real-time fluorescent quantitative polymerase chain reaction，the proliferation ability of cells in each group was detected by cell counting kit-8,the migration ability of cells in each group was detected by Transwell chamber experiment;the expressions of phosphorylated p65(P-p65)，Phosphorylated nuclear factor inhibitor protein （p-IκB），Phosphorylated c-Jun N-terminalkinae (P-JNK) and phosphorylated p38 (P-p38) protein of cells in each group were detected by Western blot.Results　There was no significant difference in the relative expression level of DHX15 mRNA，cell proliferation ability and cell migration ability of ASMCs between the control group and negative control group (P>0.05).The relative expression level of DHX15 mRNA，cell proliferation ability and cell migration ability of ASMCs in the silence group were significantly lower than those in the control group and the negative control group (P<0.05).There was no significant difference in the relative expression levels of p-p65，P-IκB，P-JNK and p-p38 of ASMCs induced by TGF-β1 between the control group and the negative control group (P>0.05).The relative expression levels of p-p65，P-IκB，P-JNK and p-p38 protein of the ASMCs in the silence group were significantly lower than those in the control group and the negative control group (P<0.05).Conclusion　Interference with the expression of DHX15 can significantly inhibit the proliferation and migration of ASMCs induced by TGF-β1，and its mechanism may be related to inhibiting the nuclear factor-κB and mitogen-activated protein kinase signaling pathway.

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更新日期/Last Update: 2021-05-05