[1]李　萍,姬白嫣,魏　娟,等.藏红花素对人宫颈癌Hela细胞顺铂耐药性的影响[J].新乡医学院学报,2021,38(5):401-405.[doi:10.7683/xxyxyxb.2021.05.001]
　LI Ping,JI Baiyan,WEI Juan,et al.Effect of crocin on cisplatin resistance of human cervical cancer Hela cells[J].Journal of Xinxiang Medical University,2021,38(5):401-405.[doi:10.7683/xxyxyxb.2021.05.001]
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藏红花素对人宫颈癌Hela细胞顺铂耐药性的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
38
期数:
2021年5
页码:
401-405
栏目:
基础研究
出版日期:
2021-05-05

文章信息/Info

Title:
Effect of crocin on cisplatin resistance of human cervical cancer Hela cells
作者:
李　萍; 姬白嫣; 魏　娟; 杜小敬; 黄　凤
（邯郸市第二医院妇产科，河北　邯郸　056000）
Author(s):
LI Ping; JI Baiyan; WEI Juan; DU Xiaojing; HUANG Feng
(Department of Obstetrics and Gynecology,the Second Hospital of Handan,Handan 056000,Hebei Province,China)
关键词:
藏红花素; 宫颈癌; Hela细胞; 顺铂; 耐药
Keywords:
crocincervical cancerHela cellscisplatinresistance
分类号:
R737.3
DOI:
10.7683/xxyxyxb.2021.05.001
文献标志码:
A
摘要:
目的　探讨藏红花素对人宫颈癌Hela细胞顺铂耐药性的影响。方法　将对数生长期耐顺铂人宫颈癌Hela/DDP细胞随机分为空白对照组、藏红花素组、顺铂组及联合组。藏红花素组细胞以含终质量浓度400 mg·L-1 藏红花素的培养基培养，顺铂组细胞以含终质量浓度5 mg·L-1顺铂的培养基培养，联合组细胞以含终质量浓度400 mg·L-1藏红花素和 5 mg·L-1顺铂的培养基培养，空白对照组细胞以正常培养基培养，每组设10个复孔。培养48 h后，采用细胞计数试剂盒-8检测各组细胞增殖抑制率，流式细胞术检测各组细胞凋亡情况和细胞周期分布，Western blot法检测各组细胞中激活型半胱氨酸蛋白酶-3（cleaved caspase-3）、B淋巴细胞瘤-2（Bcl-2）、Bcl-2相关X蛋白（Bax）、细胞周期蛋白D1（cyclin D1）、周期蛋白依赖性激酶2（CDK2）蛋白的表达。结果　与空白对照组比较，藏红花素组、顺铂组、联合组细胞增殖抑制率、细胞凋亡率、G0/G1期细胞比例及cleaved caspase-3、Bax、Bcl-2蛋白相对表达量、Bax/Bcl-2值升高（P<0.05）,g>2/M期细胞比例及cyclin D1、CDK2蛋白相对表达量降低（P<0.01）。藏红花素组细胞增殖抑制率、细胞凋亡率、g>0/G1期细胞比例、G2/M期细胞比例、Bax/Bcl-2值及cleaved caspase-3、Bax、Bcl-2、CDK2蛋白相对表达量与顺铂组比较差异均无统计学意义（P＞0.05）；与顺铂组比较，藏红花素组细胞中cyclin D1蛋白相对表达量降低（P<0.01）。与顺铂组和藏红花素组比较，联合组细胞增殖抑制率、细胞凋亡率、g>0/G1期细胞比例、Bax/Bcl-2值及cleaved caspase-3、Bax蛋白相对表达量升高（P<0.05），g>2/M期细胞比例及cyclin D1、CDK2蛋白相对表达量降低（P<0.05）。空白对照组、藏红花素组、顺铂组、联合组s期细胞比例比较差异无统计学意义（>P＞0.05）。结论　藏红花素能够逆转人宫颈癌细胞对顺铂的耐药性，其机制可能与调节凋亡相关蛋白表达、促进Hela/DDP细胞凋亡、阻滞Hela/DDP细胞周期进程有关。undefinedundefinedundefinedundefinedundefined
Abstract:
Objective　To investigate the effect of crocin on the cisplatin resistance of human cervical cancer Hela cells.Methods　The cisplatin-resistant Hela/DDP cells of human cervical cancer in logarithmic growth phase were randomly divided into blank control group,crocin group,cisplatin group and combination group;the cells in the crocin group were cultured with medium containing a final concentration of 400 mg·L-1 crocin,the cells in the cisplatin group were cultured with medium containing a final concentration of 5 mg·L-1 cisplatin,the cells in the combination group were cultured with medium containing a final concentration of 400 mg·L-1 crocin and 5 mg·L-1 cisplatin,the cells in the blank control group were cultured with normal culture medium;each group had 10 multiple holes.After 48 h of culture,the cell proliferation inhibition rate of cells in each group was detected by cell counting kit-8.The cell apoptosis and cell cycle distribution of cells in each group were detected by flow cytometry.The relative expression levels of cleaved cysteinyl aspartate specific proteinase-3 (cleaved caspase-3),B-cell lymphoma/leukemia-2 (Bcl-2),Bcl-2 associated X protein (Bax),cyclin D1,cyclin-dependent kinase 2 (CDK2) protein were detected by Western blot method.Results　Compared with the blank control group,the cell proliferation inhibition rate,apoptosis rate,G0/G1 phase cell ratio,the Bax/Bcl-2 value and the relative expression levels of cleaved caspase-3,Bax and Bcl-2 protein of cells in the crocin group,cisplatin group and combination group significantly increased (P<0.05);the ratio of G2/M phase cells and the relative expression levels of cyclin D1 and CDK2 protein significantly decreased (P<0.01).There was no significant difference in cell proliferation inhibition rate,apoptosis rate,G0/G1 phase cell ratio,G2/M phase cell ratio,Bax/Bcl-2 value and the relative expression levels of cleaved caspase-3,Bax,Bcl-2,CDK2 protein between the crocin group and the cisplatin group(P>0.05).The relative expression level of cyclin D1 protein of cells in the crocin group was significantly lower than that in the cisplatin group(P<0.01).Compared with the cisplatin group and crocin group,the cell proliferation inhibition rate,apoptosis rate,G0/G1 phase cell ratio,Bax/Bcl-2 value and the relative expression level of cleaved caspase-3,Bax protein of cells in the combination group were higher (P<0.05);the G2/M phase cells ratio and the relative expression levels of cyclin D1 and CDK2 protein were significantly lower (P<0.05).There was no significant difference in the S phase cell ratio of cells among the blank control group,crocin group,cisplatin group and combination group (P﹥0.05).Conclusion　Croctin can reverse the drug resistance of human cervical cancer cells to cisplatin,and the mechanism may be related to regulating the expression of apoptosis-related proteins,Promoting apoptosis and blocking cell cycle progression of Hela/DDP cells.

参考文献/References:

［1］　SHRESTHA A D,NEUPANE D,VEDSTED P,et al.Cervical cancer prevalence,incidence and mortality in low and middle income countries:a systematic review［J］.Asian Pac J Cancer Prev,2018,19(2):319-324.
［2］　SUN Y,LIU S,FENG Z,et al.Preoperative omental metastasis-related maximum standardized fluorine-18-fluorodeoxyglucose uptake value can predict chemosensitivity and recurrence in advanced high-grade serous ovarian cancer patients［J］.Nucl Med Commun,2018,39(8):761-767.
［3］　ZHANG W Y,LIU Y J,HE Y,et al.Suppression of long noncoding RNA NCK1-AS1 increases chemosensitivity to cisplatin in cervical cancer［J］.J Cell Physiol,2019,234(4):4302-4313.
［4］　蓝天,何俊玲,徐海滨.藏红花素抗肿瘤的研究进展［J］.浙江临床医学,2017,19(7):1372-1373.
［5］　RADAN M,DIANAT M,BADAVI M,et al.The association of cigarette smoke exposure with lung cellular toxicity and oxidative stress:the protective role of crocin［J］.Inflammation,2020,43(1):135-145.
［6］　LUO Y,CUI S,TANG F,et al.The combination of crocin with cisplatin suppresses growth of gastric carcinoma cell line BGC-823 and promotes cell apoptosis［J］.Pak J Pharm Sci,2017,30(5):1629-1634.
［7］　JIANG Z M,GU M,LIU J Q,et al.Anticancer activity of crocin against cervical carcinoma (HeLa cells):bioassessment and toxicity evaluation of crocin in male albino rats［J］.J Photochem Photobiol B,2018,180(3):118-124.
［8］　岳小丁,李秀娟,刘青,等.白藜芦醇通过诱导细胞周期阻滞及细胞凋亡增强宫颈癌细胞的化疗敏感性［J］.西安交通大学学报(医学版),2019,40(5):736-741.
［9］　TEWARI K S,SILL M W,PENSON R T,et al.Bevacizumab for advanced cervical cancer:final overall survival and adverse event analysis of a randomised,controlled,open-label,phase 3 trial (Gynecologic Oncology Group 240) ［J］.Lancet,2017,390(10103):1654-1663.
［10］　ZIELECKA-DEBSKA D,BLASZCZYK J,BLASZCZYK D,et al.The effect of the population-based cervical cancer screening program on 5-year survival in cervical cancer patients in Lower Silesia［J］.Adv Clin Exp Med,2019,28(10):1377-1383.
［11］　李文,旷雨,孟立峰,等.黄芪甲苷对宫颈癌Hela细胞增殖、迁移侵袭作用机制研究［J］.中药药理与临床,2018,34(3):39-42.
［12］　LI S R,REN B,SHI Y,et al.Notch1 inhibition enhances DNA damage induced by cisplatin in cervical cancer［J］.Exp Cell Res,2019,376(1):27-38.
［13］　HU G L,ZHANG J L,XU F F,et al.Stomatin-like protein 2 inhibits cisplatin-induced apoptosis through MEK/ERK signaling and the mitochondrial apoptosis pathway in cervical cancer cells［J］.Cancer Sci,2018,109(5):1357-1368.
［14］　任丽平,李先佳,金少举.基于caspase-3/bcl-2/bax信号通路的槐定碱诱导胰腺癌细胞株capan-1凋亡机制研究［J］.中国现代应用药学,2017,34(3):325-328.
［15］　WU D,ZHAO Y N,FU S N,et al.Seleno-short-chain chitosan induces apoptosis in human breast cancer cells through mitochondrial apoptosis pathway in vitro［J］.Cell Cycle,2018,17(13):1579-1590.
［16］　崔明宇,崔轶琼,刘媛媛,等.香叶木苷对人肝癌HepG2细胞凋亡及Bcl-2/Bax基因表达的影响［J］.药品评价研究,2018,41(11):1958-1962.
［17］　HUANG Y,FU Z,DONG W,et al.Serum starvation-induces down-regulation of Bcl-2/Bax confers apoptosis in tongue coating-related cells in vitro［J］.Mol Med Rep,2018,17(4):5057-5064.
［18］　SONG D D,LIANG H S,QU B,et al.Moxidectin inhibits glioma cell viability by inducing G0/G1 cell cycle arrest and apoptosis［J］.Oncol Rep,2018,40(3):1348-1358.

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更新日期/Last Update: 2021-05-05