[1]邹晓萍,叶豪奕,程　虎,等.脐带间充质干细胞对急性卵巢缺血再灌注损伤小鼠卵巢组织中过氧化物歧化酶及白细胞介素-12表达的影响[J].新乡医学院学报,2019,36(7):601-604.[doi:10.7683/xxyxyxb.2019.07.001]
　ZOU Xiao-ping,YE Hao-yi,CHENG Hu,et al.Effect of umbilical cord mesenchymal stem cells on the expression of superoxide dismutase and interleukin-12 in ovarian tissues of mice with acute ovarian ischemia reperfusion injury[J].Journal of Xinxiang Medical University,2019,36(7):601-604.[doi:10.7683/xxyxyxb.2019.07.001]
点击复制

脐带间充质干细胞对急性卵巢缺血再灌注损伤小鼠卵巢组织中过氧化物歧化酶及白细胞介素-12表达的影响
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
36
期数:
2019年7
页码:
601-604
栏目:
基础研究
出版日期:
2019-07-05

文章信息/Info

Title:
Effect of umbilical cord mesenchymal stem cells on the expression of superoxide dismutase and interleukin-12 in ovarian tissues of mice with acute ovarian ischemia reperfusion injury
作者:
邹晓萍1; 叶豪奕2; 程　虎2; 回月彤2; 商崇智3; 董化江2; 王　磊4; 徐　健2
（1.中国人民武装警察部队特色医学中心妇产科,天津　300162；2.中国人民武装警察部队后勤学院，天津　300189；3.中国人民武装警察部队特色医学中心神经外科，天津　300161；4.中国医学科学院北京协和医学院生物医学工程研究所，天津　300192）
Author(s):
ZOU Xiao-ping1; YE Hao-yi2; CHENG Hu2; HUI Yue-tong2; SHANG Chong-zhi3; DONG Hua-jiang2; WANG Lei4; XU Jian2
(1.Department of Gynaecology and Obstetrics,Specialty Medical Center of Chinese People＇s Armed Police Forces,Tianjin 300162,China;2.Logistics University of Chinese People＇s Armed Police Forces,Tianjin 300189,China;3.Department of Neurosurgery,Specialty Medical Center of Chinese People＇s Armed Police Forces,Tianjin 300162,China;4.Institute of Biomedical Engineering,Chinese Academy of Medical Sciences & Peking Union Medical College,Tianjin 300192,China)
关键词:
卵巢; 缺血再灌注损伤; 脐带间充质干细胞; 过氧化物歧化酶; 白细胞介素-12
Keywords:
ovarian; ischemia reperfusion injury; human umbilical cord mesenchymal stem cells; superoxide dismutase; interleukin-12
分类号:
R711.75；R-33
DOI:
10.7683/xxyxyxb.2019.07.001
文献标志码:
A
摘要:
目的　探讨脐带间充质干细胞（UC-MSC)对急性卵巢缺血再灌注损伤小鼠卵巢组织中过氧化物歧化酶（SOD）和白细胞介素-12（IL-12）表达的影响。方法　30只C57BL/6NCrl雌性小鼠随机分为假手术组、模型组及干细胞组，每组10只。假手术组小鼠仅在手术侧卵巢动脉下穿线，不结扎（不阻断血供）；模型组、干细胞组小鼠结扎手术侧卵巢动脉30 min后剪断结扎线恢复血液供应；干细胞组小鼠于恢复卵巢血液供应后24 h经尾静脉注射1.0×106个UC-MSC，假手术组、模型组小鼠均于同时间点尾静脉注射等体积的生理盐水。尾静脉注射72 h后处死各组小鼠，取手术侧卵巢组织，实时定量聚合酶链反应法检测各组小鼠卵巢组织中IL-12、SOD mRNA表达，Western blot法检测各组小鼠卵巢组织中IL-12、SOD蛋白表达。结果　模型组小鼠卵巢组织中IL-12 mRNA、IL-12蛋白表达显著高于假手术组（P<0.05）；干细胞组小鼠卵巢组织中IL-12 mRNA、IL-12蛋白表达显著低于模型组（P<0.05）。模型组和干细胞组小鼠卵巢组织中SOD mRNA、SOD蛋白表达高于假手术组（P<0.05），干细胞组小鼠卵巢组织中SOD mRNA、SOD蛋白表达高于模型组（P<0.05）。结论　UC-MSC移植可降低小鼠缺血再灌注损伤卵巢组织中IL-12表达，提高SOD表达，抑制炎症反应，改善氧化应激损伤，有益于受损卵巢功能的恢复。
Abstract:
Objective　To investigate the effect of human umbilical cord mesenchymal stem cells(UC-MSC) on the expression of superoxide dismutase (SOD) and interleukin-12(IL-12) in ovarian tissues of mice with acute ovarian ischemia reperfusion injury.Methods　Thirty C57BL/6NCrl female mice were randomly divided into sham operation group,model group and stem cell group，with 10 mice in each group.The ovarian artery of mice in the sham operation group was only threaded and not ligated;the ovarian artery of mice in the model group and stem cell group was ligated for 30 minutes,then the ligation line was cut off to restore the blood supply.The mice in the stem cell group were injected with 1.0×106 UC-MSC through tail vein at 24 hours after ovarian blood supply was restored;the mice in the sham operation group and model group were injected with the same volume of saline via tail vein at the same time point.The mice in each group were sacrificed at 72 h after tail vein injection and the ovarian tissues were taken.The expressions of IL-12 and SOD mRNA in ovarian tissues were detected by reverse transcription-polymerase chain reaction;the expressions of IL-12 and SOD protein in ovarian tissues were detected by Western blot.Results　The expressions of IL-12 mRNA and protein in ovarian tissues of mice in the model group were significantly higher than those in the sham operation group（P<0.05）;the expressions of IL-12 mRNA and protein in ovarian tissues of mice in the stem cell group were significantly lower than those in the model group（P<0.05）.The expressions of SOD mRNA and protein in ovarian tissues of mice in the model group and stem cell group were significantly higher than those in the sham operation group（P<0.05）;the expressions of SOD mRNA and protein in ovarian tissues of mice in the stem cell group were significantly higher than those in the model group（P<0.05）.Conclusion　UC-MSC transplantation can reduce the expression of IL-12,increase the expression of SOD,inhibit inflammation and improve oxidative stress injury in ovarian tissue of mice with ischemia reperfusion injury,which is beneficial to the recovery of damaged ovarian function.

参考文献/References:

［1］　单舒.二氧化碳气腹对大鼠卵巢功能影响的研究［D］.唐山：河北联合大学,2014.
［2］　张艳花.附件扭转的复位手术治疗56例临床分析［D］.杭州：浙江大学,2015.
［3］　周敏,范敏,董世庆.尼莫地平对大鼠卵巢缺血再灌注损伤后氧化应激反应的影响［J］.临床和实验医学杂志,2017,16(11):1051-1053.
［4］　邹晓萍,董化江,单娜娜,等.蕨麻正丁醇提取物对卵巢缺血再灌注损伤大鼠内皮素-1的影响［J］.新乡医学院学报,2014,31(9):682-684.
［5］　邹晓萍,董化江,单娜娜,等.蕨麻正丁醇提取物对急性卵巢缺血再灌注损伤大鼠肿瘤坏死因子-α的影响［J］.新乡医学院学报,2014,31(5):321-323.
［6］　PITTENGER M F,MACKAY A M,BECK S C,et al.Multilineage potential of adult human mesenchymal stem cells［J］.Science，1999，284(5411):143-147.
［7］　GLENN J D,WHARTENBY K A.Mesenchymal stem cells:emerging mechanisms of immunomodulation and therapy［J］.World J Stem Cells，2014，6(5):526-539.
［8］　DONG H J,SHANG C Z,LI G,et al.The distribution of transplanted umbilical cord mesenchymal stem cells in large blood vessel of rats with traumatic brain injury［J］.J Craniofac Surg，2017,28(6):1615-1619.

相似文献/References:

[1]千智斌,姬明丽,姚开辉.过氧亚硝基阴离子在脑缺血再灌注损伤中的作用[J].新乡医学院学报,2003,20(02):094.
[2]千智斌,姬明丽,姚开辉.过氧亚硝基阴离子在脑缺血再灌注损伤中的作用[J].新乡医学院学报,2003,20(02):094.
[3]元文勇,姜文泉,姜惟龙,等.大鼠肝脏短期缺血再灌注损伤早期肝细胞超微结构观察[J].新乡医学院学报,2003,20(03):161.
[4]元文勇,姜文泉,姜惟龙,等.大鼠肝脏短期缺血再灌注损伤早期肝细胞超微结构观察[J].新乡医学院学报,2003,20(03):161.
[5]张 彬１,刘慧杰２,朱绍辉１,等.氢水联合缺血后处理对大鼠小肠缺血再灌注损伤的保护作用[J].新乡医学院学报,2014,31(05):333.[doi:10.7683/xxyxyxb.2014.05.004]
[6]刘志梅１,于珊珊１,贾　佳１,等.间歇游泳训练对大鼠心肌缺血再灌注损伤的保护作用[J].新乡医学院学报,2015,32(09):817.
[7]夏　劲.丙泊酚对肝脏缺血再灌注损伤患者外周血中性粒细胞黏附因子CD11b表达的影响[J].新乡医学院学报,2017,34(2):132.[doi:10.7683/xxyxyxb.2017.02.014]
　XIA Jin.Effects of propofol on the expression of neutrophil adhesion factor CD11b in peripheral blood of patients with hepatic ischemia-reperfusion injury[J].Journal of Xinxiang Medical University,2017,34(7):132.[doi:10.7683/xxyxyxb.2017.02.014]
[8]艾　玲,乔　琼,陈　楠,等.二氢辣椒碱诱导治疗性低体温对小鼠脑缺血再灌注损伤后的脑保护作用[J].新乡医学院学报,2017,34(12):1058.[doi:10.7683/xxyxyxb.2017.12.004]
　AI Ling,QIAO Qiong,CHEN Nan,et al.Neuroprotective effect of therapeutic hypothermia induced by dihydrocapsaicin on cerebral ischemia reperfusion injury in mice[J].Journal of Xinxiang Medical University,2017,34(7):1058.[doi:10.7683/xxyxyxb.2017.12.004]
[9]王晓蕾,郭佳庆,常海宽,等.七氟烷后处理对大鼠心肌缺血再灌注损伤的影响[J].新乡医学院学报,2021,38(11):1006.[doi:10.7683/xxyxyxb.2021.11.002]
　WANG Xiaolei,GUO Jiaqing,CHANG Haikuan,et al.Effect of sevoflurane post-treatment on myocardial ischemia-reperfusion injury of rats[J].Journal of Xinxiang Medical University,2021,38(7):1006.[doi:10.7683/xxyxyxb.2021.11.002]
[10]樊　腾,李晓芳,张红伟,等.胸交感神经阻滞对大鼠心肌缺血再灌注损伤的保护作用[J].新乡医学院学报,2016,33(10):845.[doi:10.7683/xxyxyxb.2016.10.002]
　FAN Teng,LI Xiao-fang,ZHANG Hong-wei,et al.Protective effect of thoracic sympathetic nerve block on myocardial ischemia-reperfusion injury in rats[J].Journal of Xinxiang Medical University,2016,33(7):845.[doi:10.7683/xxyxyxb.2016.10.002]

更新日期/Last Update: 2019-07-05