[1]樊　腾,李晓芳,张红伟,等.胸交感神经阻滞对大鼠心肌缺血再灌注损伤的保护作用[J].新乡医学院学报,2016,33(10):845-848.[doi:10.7683/xxyxyxb.2016.10.002]
　FAN Teng,LI Xiao-fang,ZHANG Hong-wei,et al.Protective effect of thoracic sympathetic nerve block on myocardial ischemia-reperfusion injury in rats[J].Journal of Xinxiang Medical University,2016,33(10):845-848.[doi:10.7683/xxyxyxb.2016.10.002]
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胸交感神经阻滞对大鼠心肌缺血再灌注损伤的保护作用
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年10
页码:
845-848
栏目:
基础研究
出版日期:
2016-10-05

文章信息/Info

Title:
Protective effect of thoracic sympathetic nerve block on myocardial ischemia-reperfusion injury in rats
作者:
樊　腾; 李晓芳; 张红伟; 靳广丽; 马闻苛; 秦鹏宇; 岳修勤
（新乡医学院第一附属医院麻醉科，河南　卫辉　453100）
Author(s):
FAN Teng; LI Xiao-fang; ZHANG Hong-wei; JIN Guang-li; MA Wen-ke; QIN Peng-yu; YUE Xiu-qin
（Department of Anesthesiology，the First Affiliated Hospital of Xinxiang Medical University，Weihui 453100，Henan Province，China）
关键词:
胸交感神经阻滞; 缺血再灌注损伤; 心肌组织自噬
Keywords:
thoracic sympathetic nerve block; ischemia-reperfusion injury; cardiomyocyte autophagy
分类号:
R614
DOI:
10.7683/xxyxyxb.2016.10.002
文献标志码:
A
摘要:
目的　探讨胸交感神经阻滞（TSNB）对大鼠心肌缺血再灌注损伤(MIRI)的保护作用。方法　将30只健康成年雄性Wistar大鼠随机分为假手术组、心肌缺血再灌注（I/R）组和TSNB组，每组10只。TSNB组、I/R组大鼠结扎左冠状动脉前降支前分别向硬膜外隙给予体积分数0.5％罗哌卡因0.125 mL·kg-1和等量生理盐水，假手术组大鼠给予心脏左冠状动脉前降支穿线，但不结扎，穿线前硬膜外隙给予生理盐水0.125 mL·kg-1；TSNB组和I/R组大鼠均给予心脏左冠状动脉前降支穿线并结扎，40 min后解开结扎线复灌注120 min。记录各组大鼠开胸前（T0)、缺血前（T1）、缺血20 min（T2）、缺血40 min（T3）、再灌注120 min（T4）时的心率（HR）、平均动脉压（MAP），以及T0、T2~T4时血清肌钙蛋白I（cTnI）水平。再灌注120 min时2，3，5-氯三苯四唑（TTC）染色计算大鼠心肌梗死面积。Western blot法检测大鼠心肌组织自噬相关蛋白Beclin-1表达水平。结果　与组内T0时及假手术组同时间点比较，T2~T4时I/R 组和TSNB组大鼠HR、MAP显著降低（P<0.05）；与I/R组同时间点比较，T2~T4时TSNB组大鼠HR、MAP显著升高（P<0.05）。与T0时比较，T2~T4时I/R组和TSNB组大鼠血清cTnI水平显著升高（P<0.01）；与假手术组同时间点比较，T2~T4时I/R组和TSNB组大鼠血清cTnI水平显著升高（P<0.01）；T2~T3时，I/R组大鼠血清cTnI水平与TSNB组比较差异无统计学意义（P>0.05），T4时I/R组大鼠血清cTnI水平较TSNB组显著升高（P<0.01）。与 I/R 组比较，TSNB组大鼠心肌梗死区和心肌缺血危险区的百分比显著降低（P<0.01），假手术组大鼠无心肌梗死。与假手术组比较，I/R组大鼠心肌组织中Beclin-1表达显著增高（P<0.01）；与I/R组比较，TSNB组大鼠心肌组织中 Beclin-1 水平显著降低（P<0.01）。结论　胸交感神经阻滞能够减轻大鼠MIRI，其机制可能与下调心肌组织自噬因子Beclin-1表达及冠状动脉扩张有关。
Abstract:
Objective　To explore the protective effect of thoracic sympathetic nerve block（TSNB） on myocardial ischemia-reperfusion injury(MIRI) in rats.Methods　Thirty healthy adult male Wistar rats were randomly divided into sham operation group,myocardial ischemia-reperfusion (I/R) group and TSNB group,10 rats in each group.The rats in TSNB group and I/R group were given 0.5% ropivacaine (0.125 mL·kg-1) and the same amount of normal saline respectively into the epidural space before the ligation of the left anterior descending coronary artery.The rats in sham group were treated with the threading operation on the left anterior descending coronary artery,but no ligation was performed;before the threading operation,the normal saline(0.125 mL·kg-1) was injected into the epidural space.The rats in TSNB group and I/R group were treated with the ligation of the left anterior descending coronary artery;40 minutes later,the ligature was loosed for the reperfusion(120 minutes).The heart rate(HR) and meanarterial blood pressure(MAP) at the time points of the operation of opening chest (T0),before ischemia (T1),ischemia for 20 minutes (T2) and 40 minutes (T3),reperfusion for 120 minutes (T4) was recorded;and the level of serum cardiac troponin I (cTnI) at the time points of T0，T2-T4 were recorded.The area of myocardial infarction was calculated using 2,3,5-trihenylterzolium chloride;and the myocardial tissue autophagy related proteins Beclin-1 expression was dected by Western blot at the time point of reperfusion for 120 minutes.Results　Compared with the T0 and the same time points of sham group,the HR and MAP at T2-T4 in I/R group and TSNB group significantly decreased (P<0.05).Compared with I/R group,the HR and MAP at T2-T4 in TSNB group significantly increased (P<0.05).Compared with T0,the serum cTnI level at T2-T4 increased significantly in I/R group and TSNB group (P<0.01).Compared with the sham group,the serum cTnI level at T2-T4 in I/R group and TSNB group significantly increased(P<0.01).There was no significant difference in the serum cTnI level between I/R group and TSNB group at T2-T3(P> 0.01),while the serum cTnI level in I/R group was significantly higher than that in TSNB group at T4 (P<0.01).Compared with I/R group,the percentage of myocardial infarction area and myocardial ischemia danger area significantly decreased in TSNB group (P<0.01),and the rats in sham group had no myocardial infarction.The Beclin-1 expression in cardiac muscle in I/R group was significantly higher than that in sham group (P<0.01).The Beclin-1 expression in myocardial tissue in TSNB group was significantly lower than that in I/R group (P<0.01).Conclusion　The TSNB can alleviate MIRI in rats,which may be related with cardiomyocyte autophagy factor down regulation and coronary artery expansion.

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更新日期/Last Update: 2016-10-05