[1]廖婉婷,潘文疆,陈明珠,等.七叶莲对佐剂性关节炎大鼠的治疗效果及对血清白细胞介素-4、白细胞介素-10和肿瘤坏死因子-α水平的影响[J].新乡医学院学报,2023,40(2):113-118.[doi:10.7683/xxyxyxb.2023.02.003]
　LIAO Wanting,PAN Wenjiang,CHEN Mingzhu,et al.Therapeutic effect of Schefflera arboricola Hayata on adjuvant arthritis rats and its effect on the levels of serum interleukin-4,interleukin-10 and tumor necrosis factor-α[J].Journal of Xinxiang Medical University,2023,40(2):113-118.[doi:10.7683/xxyxyxb.2023.02.003]
点击复制

七叶莲对佐剂性关节炎大鼠的治疗效果及对血清白细胞介素-4、白细胞介素-10和肿瘤坏死因子-α水平的影响
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
40卷
期数:
2023年2期
页码:
113-118
栏目:
基础研究
出版日期:
2023-02-05

文章信息/Info

Title:
Therapeutic effect of Schefflera arboricola Hayata on adjuvant arthritis rats and its effect on the levels of serum interleukin-4,interleukin-10 and tumor necrosis factor-α
作者:
廖婉婷1; 潘文疆2; 陈明珠1; 林少梅1; 梁荣生1
（1.泉州医学高等专科学校药理学教研室，福建　泉州　362000；2.晋江市医院血管介入科，福建　晋江　362200）
Author(s):
LIAO Wanting1; PAN Wenjiang2; CHEN Mingzhu1; LIN Shaomei1; LIANG Rongsheng1
(1.Department of Pharmacology,Quanzhou Medical College,Quanzhou 362000,Fujian Province,China；2.Department of Vascular Intervention,Jinjiang Hospital,Jinjiang 362200,Fujian Province,China)
关键词:
七叶莲; 佐剂性关节炎; 白细胞介素-4; 白细胞介素-10; 肿瘤坏死因子-α
Keywords:
Schefflera arboricola Hayata; adjuvant arthritis; interleukin-4; interleukin-10; tumor necrosis factor-α
分类号:
R285.5
DOI:
10.7683/xxyxyxb.2023.02.003
文献标志码:
A
摘要:
目的　探讨七叶莲对佐剂性关节炎 (AA)大鼠的治疗效果及其对血清白细胞介素（IL）-4、IL-10、肿瘤坏死因子-α（TNF-α）水平的影响。
方法　将50只雄性Sprague Dawley大鼠按随机数字表法分为正常对照组、模型组、吲哚美辛组、低剂量七叶莲组、高剂量七叶莲组，每组10只。模型组、吲哚美辛组、低剂量七叶莲组、高剂量七叶莲组大鼠于左后足趾皮内注射弗氏完全佐剂100 μL制备AA模型。造模成功后，吲哚美辛组大鼠给予2.5 mg·kg-1吲哚美辛灌胃作为阳性对照，低剂量七叶莲组和高剂量七叶莲组大鼠分别给予10.0、25.0 mg·kg-1七叶莲灌胃，正常对照组和模型组大鼠给予等体积生理盐水灌胃，均每日1次，连续给药10 d。实验期间观察各组大鼠进食、饮水、皮毛、关节肿胀等一般情况。分别于造模后及给药2、5、8、10 d时，采用关节炎指数（AI）评分评估模型组、吲哚美辛组、低剂量七叶莲组、高剂量七叶莲组大鼠全身关节炎病变程度。分别于造模前、造模后及给药2、5、8、10 d时，使用游标卡尺测量各组大鼠后足跖厚度，以后足跖厚度表示大鼠足跖肿胀度。给药10 d后，采用酶联免疫吸附试验检测各组大鼠血清中IL-4、IL-10、TNF-α水平。
结果　实验期间，正常对照组大鼠进食、饮水正常，皮毛整洁，神态活泼，无关节肿胀；模型组大鼠进食、饮水量下降，活动减少，精神倦怠，局部关节肿胀明显，甚至出现溃烂；低剂量七叶莲组和高剂量七叶莲组大鼠给药6 d后，进食、饮水逐渐增加，神态良好，局部出现的溃烂开始结痂；吲哚美辛组上述表现较模型组也有改善。造模后及给药2、5 d 时，模型组、吲哚美辛组、低剂量七叶莲组、高剂量七叶莲组大鼠的AI评分比较差异无统计学意义（P>0.05）；给药8、10 d 时，吲哚美辛组、低剂量七叶莲组、高剂量七叶莲组大鼠AI评分比较差异无统计学意义（P>0.05）；给药8、10 d 时，吲哚美辛组和高剂量七叶莲组大鼠AI评分显著低于模型组（P<0.05）。造模前，5组大鼠后足跖厚度比较差异无统计学意义（P>0.05）；造模后及给药2 d时，模型组、吲哚美辛组、低剂量七叶莲组、高剂量七叶莲组大鼠后足跖厚度比较差异无统计学意义（P>0.05）；给药5、8、10 d时，吲哚美辛组、低剂量七叶莲组、高剂量七叶莲组大鼠后足跖厚度比较差异无统计学意义（P>0.05）；造模后及给药2、5、8、10 d时，模型组、吲哚美辛组、低剂量七叶莲组、高剂量七叶莲组大鼠后足跖厚度均显著大于正常对照组（P<0.05）；给药5、8、10 d 时，吲哚美辛组、高剂量七叶莲组大鼠后足跖厚度均显著低于模型组（P<0.05）；给药8 d时，低剂量七叶莲组大鼠后足跖厚度显著低于模型组（P<0.05）。模型组大鼠血清中IL-4水平显著低于正常对照组（P<0.05）；吲哚美辛组、低剂量七叶莲组、高剂量七叶莲组大鼠血清中IL-4水平显著高于模型组（P<0.05）。模型组、吲哚美辛组、低剂量七叶莲组大鼠血清中IL-10水平显著低于正常对照组（P<0.05）；吲哚美辛组、高剂量七叶莲组大鼠血清中IL-10水平显著高于模型组和低剂量七叶莲组（P<0.05）。模型组大鼠血清中TNF-α水平显著高于正常对照组和高剂量七叶莲组（P<0.05）。
结论　高剂量七叶莲可显著改善AA大鼠的关节炎病变程度和足肿胀度，作用机制可能与其调控血清中IL-4、IL-10、TNF-α水平有关。
Abstract:
Objective　To investigate the therapeutic effect of Schefflera arboricola Hayata on adjuvant arthritis (AA) rats and its effect on serum interleukin (IL)-4,IL-10 and tumor necrosis factor-α (TNF-α) levels.
Methods　Fifty male Sprague Dawley rats were divided into the normal control group,model group,indomethacin group,low-dose Schefflera arboricola Hayata group and high-dose Schefflera arboricola Hayata group according to random number table method,with 10 rats in each group.Rats in the model group,indomethacin group,low-dose Schefflera arboricola Hayata group and high-dose Schefflera arboricola Hayata group were intradermally injected with 100 μL Freund′s complete adjuvant on the left hind toe to prepare the AA model.After successful modeling constructed,the rats in the indomethacin group were given 2.5 mg·kg-1 indomethacin by gavage as a positive control,the rats in the low-dose Schefflera arboricola Hayata group and high-dose Schefflera arboricola Hayata group were given 10 and 25 mg · kg-1 Schefflera arboricola Hayata by gavage,and the rats in the normal control group and model group were given the same volume of normal saline by gavage,once a day for 10 consecutive days.During the experiment,the general conditions of rats in each group were observed,such as eating,drinking,fur,joint swelling,etc.After modeling and 2,5,8,10 days of administration,the degree of systemic arthritis of rats in the model group,indomethacin group,low-dose Schefflera arboricola Hayata group and high-dose Schefflera arboricola Hayata group was evaluated by the arthritis index (AI) score.Before and after modeling,and 2,5,8 and 10 days of administration,the thickness of the hind paw of rats in each group was measured with vernier caliper,and the thickness of the hind paw represented the degree of swelling of the rat′s foot.After 10 days of administration,the levels of serum IL-4,IL-10 and TNF-α of rats in each group were detected by enzyme-linked immunosorbent assay.
Results　During the experiment,the rats in the normal control group ate and drink normally,the fur was clean,the appearance was lively,and there was no joint swelling;in the model group,the amount of food and water consumption decreased,the activity decreased,the mental fatigue,local joint swelling was obvious,and even ulceration occurred;after 6 days of administration,the rats in the low-dose Schefflera arboricola Hayata group and high-dose Schefflera arboricola Hayata group eating and drinking gradually increased,their mien were good,and local ulceration began to scab;the above performance of rats in the indomethacin group were also improved compared with the model group.There was no significant difference in AI scores of rats among the model group,indomethacin group,low-dose Schefflera arboricola Hayata group and high-dose Schefflera arboricola Hayata group after model establishment and at 2 and 5 days of administration (P>0.05);at 8 and 10 days of administration,there was no significant difference in AI scores among the indomethacin group,low-dose Schefflera arboricola Hayata group and high-dose Schefflera arboricola Hayata group (P>0.05);at 8 and 10 days of administration,the AI scores of rats in the indomethacin group and high-dose Schefflera arboricola Hayata group were significantly lower than those in the model group (P<0.05).Before modeling,there was no significant difference in the thickness of hind foot among the five groups (P>0.05);after model establishment and at 2 days of administration,there was no significant difference in the thickness of hind foot among the model group,indomethacin group,low-dose Schefflera arboricola Hayata group and high-dose Schefflera arboricola Hayata group (P>0.05);at 5,8 and 10 days of administration,there was no significant difference in the thickness of hind foot among the indomethacin group,low-dose Schefflera arboricola Hayata group and high-dose Schefflera arboricola Hayata group (P>0.05);after model establishment and at 2,5,8 and 10 days of administration,the thickness of hind foot of rats in the model group,indomethacin group,low-dose Schefflera arboricola Hayata group and high-dose of Schefflera arboricola Hayata group was significantly higher than that in the normal control group (P<0.05);at 5,8 and 10 days of administration,the thickness of hind foot of rats in the indomethacin group and high-dose Schefflera arboricola Hayata group was significantly lower than that in the model group (P<0.05);at 8 days of administration,the thickness of hind food of rats in the low-dose Schefflera arboricola Hayata group was significantly lower than that in the model group (P<0.05).The level of IL-4 in serum of rats in the model group was significantly lower than that in the normal control group (P<0.05);the level of IL-4 in serum of rats in the indomethacin group,low-dose Schefflera arboricola Hayata group and high-dose Schefflera arboricola Hayata group was significantly higher than that in the model group (P<0.05).The level of IL-10 in serum of rats in the model group,indomethacin group and low-dose Schefflera arboricola Hayata group was significantly lower than that in the normal control group (P<0.05);the level of IL-10 in serum of rats in the indomethacin group and high-dose Schefflera arboricola Hayata group was significantly higher than that in the model group and low-dose Schefflera arboricola Hayata group (P<0.05).The level of TNF-α in serum of rats in the model group was significantly higher than that in the normal control group and the high-dose Schefflera arboricola Hayata group (P<0.05).
Conclusion　High-dose of Schefflera arboricola Hayata can significantly improve the degree of arthritis and foot swelling in AA rats.The mechanism may be related to its effect on regulation of IL-4,IL-10 and TNF-α level.

参考文献/References:

［1］　UHLIG T,MOE R H,KVIEN T K.The burden of disease in rheumatoid arthritis［J］.Pharmacoeconomics,2014,32(9):841-851.
［2］　KRONZER V L,DAVIS J M.Etiologies of rheumatoid arthritis:update on mucosal,genetic,and cellular pathogenesis［J］.Curr Rheumatol Rep,2021,23(4):21.
［3］　OHGI K,KAJIYA H,GOTO-T K,et al.Toll-like receptor 2 activation primes and upregulates osteoclastogenesis via lox-1［J］.Lipids Health Dis,2018,17(1):132.
［4］　苏雨荷，王刚，文振华，等.类风湿关节炎的发病机制及药物治疗研究进展［J］.西北药学杂志,2021,36(5)：857-861.SU Y H,WANG G,WEN Z H,et al.Research progress on the pathogensis and drug treatment of rheumatoid arthritis［J］.J Northwest Pharm,2021,36(5):857-861.
［5］　祁芳,李艳玲,艾坤,等.SD大鼠佐剂性关节炎模型的建立与评估［J］.湖南中医药大学学报,2016,36(1):23-26.QI F,LI Y L,AI K,et al.The establishment and evaluation of adjuvant-induced arthritis in SD rats［J］.J Hunan Univ Chin Med,2016,36(1):23-26.
［6］　孙爱静,徐先祥,黄晓东,等.七叶莲花抗炎镇痛作用及机制研究［J］.中药材,2014,37(2):311-315.SUN A J,XU X X,HUANG X D,et al.Studies on the anti-infla-mmatory and analgesic effects of Schefflera arboricola Hayata and its mechanism［J］.J Chin Med Mater,2014,37(2):311-315.
［7］　林小凤,张慧,隋臻,等.七叶莲不同溶剂提取部分的抗炎镇痛作用［J］.中国生化药物杂志，2012，33(4):346-349.LIN X F,ZHANG H,SUI Z,et al.Anti-inflammatory and analgesic activities of compounds isolated from the Schefflera arboricola Hayata with different solvents［J］.Chin J Biochem Pharm,2012,33(4):346-349.
［8］　KUMAR A,DHALIWAL N,DHALIWAL J,et al.Astaxanthin attenuates oxidative stress and inflammatory responses in complete Freund-adjuvant-induced arthritis in rats［J］.Pharmacol Rep,2019,72(1):104-114.
［9］　DARAM P,JITTA S R,SHREEDHARA C S,et al.Investigation of anti-inflammatory and anti-arthritic potentials of terminalia catappa bark using in vitro assays and carrageenan-induced inflammation,complete Freund′s adjuvant induced arthritis model in rats［J］.S Afr J Bot,2021,141:313-321.
［10］中国医科院药用植物研究所.中药学［M］.北京:人民卫生出版社,1994:561-565.INSTITUTE OF MEDICINAL PLANTS,CHINESE ACADEMY OF MEDICAL SCIENCES.Science of Chinese pharmacology［M］.Beijing:People′s Medical Publishing House,1994:561-565.
［11］　刘同祥,张艳萍.七叶莲的研究进展［J］.中央民族大学学报（自然科学版）,2010,19(2):75-77.LIU T X,ZHANG Y P.Research progress on Schefflera arboricola root［J］.J Minzu Univ China (Nat Sci Edit),2010,19(2):75-77.
［12］　杨海波.疏风胜湿汤联合吲哚美辛治疗类风湿性关节炎随机平行对照研究［J］.实用中医内科杂志，2015,29(1):95-97.YANG H B.Expelling wind and dampness decoction combined with indomethacin random parallel controlled study on treatment of rheumatoid arthritis［J］.J Pract Trad Chin Int Med,2015,29(1):95-97.
［13］　廖婉婷，潘文疆，蔡聪艺，等.七叶莲对佐剂性关节炎大鼠的治疗作用［J］.海峡药学,2018,22（6）:25-27.LIAO W T,PAN W J,CAI C Y,et al.Therapeutic effect of Paris polyphylla Simth in Freund′s complete adjuvant arthritis rats［J］.Strait Pharm J,2018,22(6):25-27.
［14］　杨渐，张晓昕，刘倩，等.钩吻素子对佐剂诱导关节炎大鼠T辅助细胞因子平衡偏移的调节作用［J］.中国药理学与毒理学杂志,2018,32(9):719-725.YANG J,ZHANG X X,LIU Q,et al.Regulation of Koumine on the balance shift of T helper cytokines in rats with adjuvant-induced arthritis［J］.Chin J Pharmacol Toxicol,2018,32(9):719-725.
［15］　DONG C,FU T,JI J,et al.The role of interleukin-4 in rheumatic diseases［J］.Clin Exp Pharmacol Physiol,2018,45(8):747-754.
［16］　李伟，王佳怡，肖春媛，等.IL-4通过促进CD30分子表达抑制类风湿关节炎患者T细胞功能的研究［J］.同济大学学报(医学版),2021,42(4):528-533.LI W,WANG J Y,XIAO C Y,et al.IL-4 inhibits T cell function in patients with rheumatoid arthritis through promoting CD30 expression［J］.J Tongji Univ (Med Sci),2021,42(4):528-533.
［17］　SUN Y H,WEI S T,ZONG S H,et al.Correlation between IL-4 gene polymorphismas well as its mRNA expression and rheumatoid arthritis［J］.Eur Rev Med Pharmacol Sci,2017,21(17):3879-3885.
［18］　TAKASUGI K,YAMA M,IWAHASHI M,et al.Induction of tumour necrosis factor receptor-expressing macrophages by interleukin-10 and macrophage colony-stimulating factor in rheumatoid arthritis［J］.Arthritis Res Ther,2006,8(4):R126.
［19］　JOHNSON J L,JONES M B,COBB B A,et al.Polysaccharide-experienced effector T cells induce IL-10 in FoxP3+ regulatory T cells to prevent pulmonary inflammation［J］.Glycobiology,2018,28(1):50-58.
［20］　周惠敏,胡志刚.白细胞介素-10抑制类风湿关节炎患者的研究进展［J］.医疗装备,2019,32(23):197-199.ZHOU H M，HU Z G.Research progress of interleukin-10 inhibition in patients with rheumatoid arthritis［J］.Med Equipm,2019,32(23):197-199.
［21］　BJARNADTTIR U,EINARSDTTIR H K,STEFNSDTTIR E,et al.Resolution of Th/Tc17-driven inflammation during anti-TNFα treatment of rheumatoid arthritis reveals a unique immune biomarker profiling pattern［J］.Scand J Immunol,2021,95(1):e13116.

更新日期/Last Update: 2023-02-05