[1]张李娟,赵舒祥,王若溪,等.淀粉样前体蛋白/早老素1转基因小鼠脑皮质中过氧化物酶体增殖剂激活受体α的表达及意义[J].新乡医学院学报,2022,39(9):801-805.[doi:10.7683/xxyxyxb.2022.09.001]
　ZHANG Lijuan,ZHAO Shuxiang,WANG Ruoxi,et al.Expression and significance of peroxisome proliferator-activated receptor alpha in cerebral cortex of amyloid precursor protein/presenilin 1 transgenic mice[J].Journal of Xinxiang Medical University,2022,39(9):801-805.[doi:10.7683/xxyxyxb.2022.09.001]
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淀粉样前体蛋白/早老素1转基因小鼠脑皮质中过氧化物酶体增殖剂激活受体α的表达及意义
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
39
期数:
2022年9
页码:
801-805
栏目:
基础研究
出版日期:
2022-09-05

文章信息/Info

Title:
Expression and significance of peroxisome proliferator-activated receptor alpha in cerebral cortex of amyloid precursor protein/presenilin 1 transgenic mice
作者:
张李娟1; 赵舒祥1; 王若溪1; 卜勇军1; 杨　磊2; 原志庆3; 李文强4; 石如玲1; 4
（1.新乡医学院公共卫生学院，河南　新乡　453003；2.新乡医学院护理学院，河南　新乡　453003；3.新乡医学院基础医学院，河南　新乡　453003；4.河南省生物精神病学重点实验室，河南　新乡　453002）
Author(s):
ZHANG Lijuan1; ZHAO Shuxiang1; WANG Ruoxi1; BU Yongjun1; YANG Lei2; YUAN Zhiqing3; LI Wenqiang4; SHI Ruling1; 4
（1.School of Public Health,Xinxiang Medical University,Xinxiang 453003,Henan Province,China;2.School of Nursing,Xinxiang Medical University,Xinxiang 453003,Henan Province,China;3.Basic Medical College,Xinxiang Medical University,Xinxiang 453003,Henan Province,China;4.Henan Key Laboratory of Biological Psychiatry,Xinxiang 453002,Henan Province,China）
关键词:
淀粉样前体蛋白/早老素1转基因小鼠; 阿尔茨海默病; 过氧化物酶体增殖剂激活受体α; 脂质代谢
Keywords:
amyloid precursor protein/presenilin 1 transgenic mice; Alzheimer′s disease; peroxisome proliferator-activated receptor alpha; lipid metabolism
分类号:
R741
DOI:
10.7683/xxyxyxb.2022.09.001
文献标志码:
A
摘要:
目的　探讨淀粉样前体蛋白（APP）/早老素1（PS1）转基因小鼠脑皮质中过氧化物酶体增殖剂激活受体α（PPARα）的表达变化及意义。方法　选择10只5月龄雄性APP/PS1双转基因C57BL/6J-TgN（APP/PS1）ZLFILAS小鼠为实验组，10只同遗传背景5月龄雄性C57BL/6J野生型小鼠为对照组。采用Morris水迷宫实验测定2组小鼠空间学习记忆能力，免疫组织化学法检测2组小鼠脑组织中β-淀粉样蛋白（Aβ）沉积情况，Western blot法检测2组小鼠脑皮质中 PPARα、APP蛋白及肝组织中PPARα、脂酰辅酶A氧化酶1（ACOX1）及肉碱脂酰转移酶1（CPT1）蛋白表达，生物化学法检测2组小鼠血清中三酰甘油（TG）和总胆固醇（TC）水平。结果　实验组小鼠不同时间点逃避潜伏期短于对照组，但2组间比较差异无统计学意义（P>0.05）；实验组小鼠在目标象限游动时间短于对照组，但2组间比较差异无统计学意义（P>0.05）。对照组小鼠脑组织中未见到Aβ阳性斑块，实验组小鼠脑皮质和海马区可见大量大小不等的Aβ阳性斑块。实验组小鼠脑皮质中PPARα蛋白相对表达量显著低于对照组，APP蛋白相对表达量显著高于对照组（P<0.05）。实验组小鼠肝组织中PPARα、ACOX1、CPT1蛋白相对表达量显著高于对照组（P<0.05）。实验组小鼠血清TG水平显著高于对照组（P<0.05），实验组与对照组小鼠血清TC水平比较差异无统计学意义（P>0.05）。结论　APP/PS1转基因小鼠存在脑组织和肝组织PPARα异常表达、脂质代谢紊乱，PPARα在阿尔茨海默病的发病机制中起重要作用。
Abstract:
Objective　To investigate the expression and significance of peroxisome proliferator-activated receptor alpha (PPARα) in cerebral cortex of amyloid precursor protein (APP)/presenilin 1 (PS1) transgenic mice.Methods　Ten 5-month-old male APP/PS1 double-transgenic C57BL/6J-TgN (APP/PS1) ZLFILAS mice were selected as the experimental group,and ten 5-month-old male C57BL/6J wild-type mice with the same genetic background were selected as the control group.The spatial learning and memory ability of mice in the two groups was determined by Morris water maze,the deposition of β-amyloid (Aβ) in brain tissues of mice in the two groups was determined by immunohistochemistry,the expressions of PPARα,APP protein in cerebral cortex and PPARα,fatty acyl-CoA oxidase 1 (ACOX1),carnitine fatty acyltransferase 1 (CPT1) in liver tissues of mice in the two groups were determined by Western blot,and the levels of serum triglyceride (TG) and total cholesterol (TC) of mice in the two groups were detected by biochemical method.Results　The escape latency of mice in the experimental group was shorter than that in the control group at different time points,but there was no significant difference in the escape latency of mice between the two groups (P>0.05).The swimming time in the target quadrant of mice in the experimental group was shorter than that in the control group,but there was no significant difference in the swimming time of mice between the two groups (P>0.05).Aβ-positive plaque was not found in the brain tissue of mice in the control group,while a large number of Aβ-positive plaques with varying sizes were found in the cerebral cortex and hippocampus of mice in the experimental group.The relative expression level of PPARα protein in the cerebral cortex in the experimental group was significantly lower than that in the control group,and the relative expression level of APP protein was significantly higher than that in the control group (P<0.05).The relative expression levels of PPARα,ACOX1 and CPT1 protein in the liver tissue of mice in the experimental group were significantly higher than those in the control group (P<0.05).The level of serum TG of mice in the experimental group was significantly higher than that in the control group (P<0.05),and there was no significant difference in the level of serum TC of mice between the experimental group and the control group (P>0.05).Conclusion　APP/PS1 transgenic mice have abnormal expression of PPARα in brain and liver tissues and disorder of lipid metabolism,PPARα may play an important role in the pathogenesis of Alzheimer′s disease.

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更新日期/Last Update: 2022-09-05