[1]弓慧杰,张光明.高糖环境下西格列汀对大鼠心肌细胞纤维化过程中转录激活蛋白-1及骨膜蛋白表达的影响[J].新乡医学院学报,2020,37(9):830-834.[doi:10.7683/xxyxyxb.2020.09.005]
　GONG Huijie,ZHANG Guangming.Effect of sitagliptin on the expression of transcriptional activator-1 and periostinin in the process of cardiomyocyte fibrosis under the environment of high sugar[J].Journal of Xinxiang Medical University,2020,37(9):830-834.[doi:10.7683/xxyxyxb.2020.09.005]
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高糖环境下西格列汀对大鼠心肌细胞纤维化过程中转录激活蛋白-1及骨膜蛋白表达的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
37
期数:
2020年9
页码:
830-834
栏目:
基础研究
出版日期:
2020-09-05

文章信息/Info

Title:
Effect of sitagliptin on the expression of transcriptional activator-1 and periostinin in the process of cardiomyocyte fibrosis under the environment of high sugar
作者:
弓慧杰1; 张光明2
（1.成都市新都区人民医院内分泌/肾内科，四川　成都　610500；2.西南医科大学病理生理学研究室，四川　泸州　646000）
Author(s):
GONG Huijie1; ZHANG Guangming2
(1.Department of Endocrinology and Nephrology,Xindu District People′s Hospital of Chengdu City,Chengdu 610500,Sichuan Province,China;2.Department of Pathophysiology,Southwest Medical University,Luzhou 646000,Sichuan Province,China)
关键词:
西格列汀; 大鼠心肌细胞; 心肌细胞纤维化; 骨膜蛋白; 转录激活蛋白-1
Keywords:
sitagliptin; rat cardiomyocyte; cardiomyocyte fibrosis; periostin; transcriptional activator protein-1
分类号:
R542.23
DOI:
10.7683/xxyxyxb.2020.09.005
文献标志码:
A
摘要:
目的　探讨在高糖环境下西格列汀对大鼠心肌细胞纤维化过程中骨膜蛋白（POSTN）及转录激活蛋白-1表达（AP-1）的影响。方法　将大鼠心肌细胞H9c2复苏后接种于含体积分数10%胎牛血清的改良型洛斯维1640培养基持续培养，并用无血清培养基继续培养使其同步化；将同步化的H9c2细胞分为对照组、高糖损伤组及西格列汀处理组，对照组H9c2细胞予以普通培养液，高糖损伤组H9c2细胞予以葡萄糖浓度为33.0 mmol·L-1的培养基，西格列汀处理组H9c2细胞予以终浓度为33.0 mmol·L-1葡萄糖+0.1 μmol·L-1西格列汀的培养基。采用细胞计数试剂盒-8检测细胞活力。酶联免疫吸附试验检测H9c2细胞上清液中Ⅲ型胶原蛋白（COL Ⅲ）表达水平；免疫组织化学法检测H9c2细胞中AP-1蛋白表达水平；反转录-聚合酶链反应检测H9c2细胞中POSTN和AP-1 mRNA表达水平。结果　高糖损伤组和西格列汀处理组H9c2细胞增殖活力均显著高于对照组（P<0.05）；西格列汀处理组H9c2细胞增殖活力显著低于高糖损伤组（P<0.05）。高糖损伤组和西格列汀处理组H9c2细胞中COL Ⅲ表达水平均显著高于对照组（P<0.05）；西格列汀处理组H9c2细胞中COL Ⅲ表达水平均显著低于高糖损伤组（P<0.05）。高糖损伤组和西格列汀处理组H9c2细胞中AP-1表达水平显著高于对照组（P<0.05）；西格列汀处理组H9c2细胞中AP-1表达水平显著低于高糖损伤组（P<0.05）。高糖损伤组和西格列汀处理组H9c2细胞中POSTN、AP-1 mRNA表达水平均显著高于对照组（P<0.05）；西格列汀处理组H9c2细胞中POSTN、AP-1 mRNA表达水平均显著低于高糖损伤组（P<0.05）。结论　体外高糖环境可促进大鼠心肌细胞的增殖活力，并使心肌细胞纤维化；西格列汀可通过抑制H9c2细胞中AP-1的过表达下调POSTN的表达，从而减缓纤维化进程，延缓心肌细胞损伤。
Abstract:
Objective　To investigate the effect of sitagliptin on the expression of periostin (POSTN) and transcriptional activator protein-1 (AP-1) in the process of cardiomyocyte fibrosis under a high glucose environment.Methods　Rat′s cardiomyocyte cells (H9c2 cells) were inoculated with 10% fetal bovine serum modified park roswell memorial institute 1640 medium for continuous culture after resuscitation,and then were further cultured in serum-free medium for synchronization;the synchronized cardiomyocytes were divided into the control group,the high glucose injury group and the sitagliptin treatment group.H9c2 cells in the control group was cultured in a normal medium,H9c2 cells in the high glucose injury group were cultured in a medium with concentration of 33.0 mmol·L-1 glucose,and H9c2 cells in the sitagliptin treatment group were cultured in a medium with 33.0 mmol·L-1 glucose and 0.1 μmol·L-1 sitagliptin.The cell activity was measured by cell counting kit-8 method.Enzyme linked immunosorbent assay was used to detect the expression level of type Ⅲ collagen(COL Ⅲ) in the supernatant of H9c2 cells;immunohistochemistry was used to detect the expression of AP-1 in H9c2 cells;reverse transcription-polymerase chain reaction was used to detect the expression of POSTN and AP-1 mRNA in H9c2 cells.Results　The proliferation activity of H9c2 cells in the high glucose injury group and the sitagliptin treatment group was significantly higher than that in the control group (P<0.05),while the proliferation activity of H9c2 cells in the sitagliptin treatment group was significantly lower than that in the high glucose injury group(P<0.05).The expression of COL Ⅲ in the H9c2 cells in the high glucose injury group and the sitagliptin treatment group was significantly higher than that in the control group(P<0.05),and the expression of COL Ⅲ of H9c2 cells in the sitagliptin treatment group was significantly lower than that in the high glucose injury group(P<0.05).The expression levels of POSTN and AP-1 mRNA in the high glucose injury group and the sitagliptin treatment group were significantly higher than those in the control group (P<0.05),and the expression levels of POSTN and AP-1 mRNA in the sitagliptin treatment group were significantly lower than those in the high glucose injury group (P<0.05).Conclusion　High glucose environment can promote the proliferation activity of rat′s cardiomyocytes and make the cardiomyocytes fibrosis.Sitagliptin can down-regulate the expression of POSTN by inhibiting the overexpression of AP-1 in H9c2 cells,and then slow down the process of fibrosis and delay the damage of cardiomyocytes.

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更新日期/Last Update: 2020-09-05