[1]黄永超,李慧君,安　珍,等.新乡市大气细颗粒物中细菌种群多样性及分布特征[J].新乡医学院学报,2019,36(7):615-618.[doi:10.7683/xxyxyxb.2019.07.004]
　HUANG Yong-chao,LI Hui-jun,AN Zhen,et al.Biodiversity and distribution characteristics of bacteria in atmospheric particulate matter in Xinxiang city[J].Journal of Xinxiang Medical University,2019,36(7):615-618.[doi:10.7683/xxyxyxb.2019.07.004]
点击复制

新乡市大气细颗粒物中细菌种群多样性及分布特征
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
36
期数:
2019年7
页码:
615-618
栏目:
基础研究
出版日期:
2019-07-05

文章信息/Info

Title:
Biodiversity and distribution characteristics of bacteria in atmospheric particulate matter in Xinxiang city
作者:
黄永超; 李慧君; 安　珍; 徐光翠; 范　威; 单怡凡; 姜　静; 张丰泉; 李海斌; 吴　辉; 吴卫东
（新乡医学院公共卫生学院，河南省国际联合实验室，河南 　新乡　453003）
Author(s):
HUANG Yong-chao; LI Hui-jun; AN Zhen; XU Guang-cui; FAN Wei; SHAN Yi-fan; CHAO Ling; ZHANG Feng-quan; LI Hai-bin; WU Hui; WU Wei-dong
（School of Public Health，Xinxiang Medical University,Henan International Collaborative Laboratory for Health Effects and Intervention of Air Pollution,Xinxiang 453003，Henan Province,China）
关键词:
大气污染; 细颗粒物; 细菌种群; 高通量测序
Keywords:
air pollution; particulate matter; bacteria community; high-throughput sequencing technology
分类号:
R122
DOI:
10.7683/xxyxyxb.2019.07.004
文献标志码:
A
摘要:
目的　研究新乡市大气细颗粒物（PM2.5）中细菌种群多样性及分布特征。方法　采集4个季节PM2.5样品，利用高通量测序技术分析种群结构及其多样性。结果　冬季大气中PM2.5浓度最高，其次为春季、秋季和夏季。在细菌门的水平上，4个季节相对丰度最高的是变形菌门。春季和夏季丰度相对较高的菌属均为乳杆菌属，秋、冬季丰度相对较高的菌属分别为未鉴定线粒体、未鉴定叶绿体。4个季节大气PM2.5中细菌群落相对丰度依次为春季>冬季>夏季>秋季，4个季节大气PM2.5中群落多样性依次为夏季>春季>冬季>秋季。春季、夏季、秋季大气PM2.5中细菌群落Beta多样性指数两两比较差异均有统计学意义（P<0.05），春季和冬季大气PM2.5中细菌群落Beta多样性指数比较差异无统计学意义（P>0.05）。结论　新乡市大气PM2.5中细菌种群具有显著的季节变化特征，春季和冬季PM2.5中细菌丰度也相对高于夏季和秋季。
Abstract:
Objective　To study the iodiversity and distribution characteristics of bacteria in atmospheric particulate matter （PM2.5）in Xinxiang.Methods　PM2.5 samples in four seasons were collected and its bacterial biodiversity and community structure were analyzed by using high throughput sequencing technology.Results　PM2.5 concentration was highest in the winter,followed by the spring,autumn and summer.At bacteriophyta level,Proteobacteria was the most abundant phylum in PM2.5 samples in the four seasons.Lactobacillus was higher in relative abundant in PM2.5 samples in the spring and summer,and unidentified mitochondria,unidentified chloroplast were relatively higher in abundance in the autumn and winter.The order of bacterial abundance of PM2.5 in the four seasons were spring>winter>summer>autumn,while the bacterial biodiversity of PM2.5 in the four seasons were summer>spring>autumn>winter.There was statistic difference in the Beta diversity index of bacterial community in PM2.5 among spring,summer and autumn (P<0.05);there was no statistic difference in the Beta diversity index of bacterial community in PM2.5 between the spring and winter（P>0.05）.Conclusion　The bacterial biodiversity of the PM2.5 samples significantly varies with season.The bacterial abundance in spring and winter are relatively higher than summer and autumn.

参考文献/References:

［1］　冯建纯,孙红梅,杜月菊,等.石家庄市PM2.5浓度对居民死亡的急性影响分析［J］.现代预防医学,2018,45(4):603-608.
［2］　BURNETT R T,POPE C A III,EZZATI M,et al.An integrated risk function for estimating the global burden of disease attributable to ambient ne particulate matter exposure［J］.Environ Health Perspect，2014，122(4):397-403.
［3］　BI J R,HUANG J P,HU Z Y,et al.Investigating the aerosoloptical and radiative characteristics of heavy haze episodes in Beijing during January of 2013［J］.J Geophys Res Atmos,2014,119(16):9884-9900.
［4］　田大年,丁润梅,张鹏举,等.银川市大气PM2.5 和PM10 中多环芳烃的污染特征［J］.现代预防医学,2017,44(20):3672-3676.
［5］　JAENICKE R.Abundance of cellular material and proteins in thea-tmosphere［J］.Science,2005,308(5718):73.
［6］　李慧君,李宏彬,王艳,等.大气颗粒物中微生物群落多样性及危害研究进展［J］.新乡医学院学报,2015,32(2):107-110.
［7］　王步英,郎继东,张丽娜,等.基于16S rRNA 基因测序法分析北京霾污染过程中PM2.5和PM10细菌群落特征［J］.环境科学,2015,36(8):2727-2734.
［8］　BOVALLIUS A,BUCHT B,ROFFEY R,et al.Three-year inve-stigation of the natural airborne bacterial flora at four localities in sweden［J］.Appl Environ Microbiol,1978,35(5):847-852.
［9］　PECCIA J,HERNANDEZ M.Incorporating polymerase chain reac-tionbased identification,population characterization and quantification of microorganisms into aerosol science:a review［J］.Atmospheric Environment,2006,40(21):3941-3961.
［10］　LUNDBERG D,DEREK S,MIECZKOWSKI P,et al.Practica-linnovations for high-throughput amplicon sequencing［J］.Nat Methods,2013,10（10）:999-1002.
［11］　WANG H,NAGHAVI M,ALLEN C,et al.Global,regional,and national life expectancy,all-cause mortality,and cause-specific mortality for 249 causes of death,1980-2015:a systematic analysis for the Global Burden of Disease Study 2015［J］.Lancet,2016,388(10053):1459-1544.
［12］　李文静,张美云,万博宇,等.北京市大气 PM2.5污染对某医院儿科门诊量影响的时间序列研究［J］.现代预防医学，2018,45（7）：1184-1188.

相似文献/References:

[1]晋乐飞１,冯斐斐１,吴卫东. 大气细颗粒污染物对呼吸系统危害研究进展 [J].新乡医学院学报,2015,32(02):091.[doi:10.7683/xxyxyxb.2015.02.001]
[2]李　航,郭伟丽,安　珍,等.PM2.5对慢性阻塞性肺疾病影响研究进展[J].新乡医学院学报,2016,33(3):234.[doi:10.7683/xxyxyxb.2016.03.020]
　LI Hang,GUO Weili,AN Zhen,et al.Research progress on the effect of PM2.5 on chronic obstructive pulmonary disease[J].Journal of Xinxiang Medical University,2016,33(7):234.[doi:10.7683/xxyxyxb.2016.03.020]
[3]孙贝贝,田　歌,吴卫东.大气颗粒物所致健康效应的易感因素[J].新乡医学院学报,2020,37(8):797.[doi:10.7683/xxyxyxb.2020.08.022]
[4]高丽云,张学军,汪　涛,等.环境中细颗粒物PM2.5对呼吸系统的作用机制[J].新乡医学院学报,2017,34(3):163.[doi:10.7683/xxyxyxb.2017.03.001]

更新日期/Last Update: 2019-07-05