[1]黄琰菁,王　琳,李　赛,等.长链非编码RNA PCGEM1对肝癌Huh7细胞增殖、侵袭及凋亡的影响[J].新乡医学院学报,2019,36(5):406-410.[doi:10.7683/xxyxyxb.2019.05.002]
　HUANG Yan-jing,WANG Lin,LI Sai,et al.Effects of long noncoding RNA PCGEM1 on proliferation，invasion and apoptosis of hepatoma carcinoma Huh7 cells[J].Journal of Xinxiang Medical University,2019,36(5):406-410.[doi:10.7683/xxyxyxb.2019.05.002]
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长链非编码RNA PCGEM1对肝癌Huh7细胞增殖、侵袭及凋亡的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
36
期数:
2019年5
页码:
406-410
栏目:
基础研究
出版日期:
2019-05-05

文章信息/Info

Title:
Effects of long noncoding RNA PCGEM1 on proliferation，invasion and apoptosis of hepatoma carcinoma Huh7 cells
作者:
黄琰菁; 王　琳; 李　赛; 盛　莉; 王海霞; 杨生辉
（海南省人民医院肿瘤内科，海南　海口　570311）
Author(s):
HUANG Yan-jing; WANG Lin; LI Sai; SHENG Li; WANG Hai-xia; YANG Sheng-hui
（Department of Oncology，Hainan Provincial People′s Hospital，Haikou 570311，Hainan Province，China）
关键词:
非编码RNA PCGEM1; 肝癌细胞; 增殖; 侵袭; 凋亡
Keywords:
long noncoding RNA; hepatoma carcinoma cell; proliferation; invasion; apoptosis
分类号:
R605
DOI:
10.7683/xxyxyxb.2019.05.002
文献标志码:
A
摘要:
目的　探讨长链非编码RNA（LncRNA）PCGEM1对肝癌细胞增殖、侵袭及凋亡的影响。方法　构建LncRNA PCGEM1慢病毒沉默表达载体，培养人肝癌细胞系Huh7细胞至对数生长期，分为空白对照组、阴性对照组和观察组，空白对照组细胞未进行转染，阴性对照组细胞应用空慢病毒载体进行转染，观察组细胞应用LncRNA PCGEM1慢病毒沉默表达载体进行转染。采用实时荧光定量聚合酶链反应检测各组肝癌细胞中LncRNA PCGEM1及Caspase-3、Caspase-9 mRNA表达，细胞计数试剂盒-8检测细胞的增殖能力，Transwell小室实验检测细胞的迁移能力，流式细胞术检测细胞的凋亡情况。结果　3组细胞中LncRNA PCGEM1及Caspase-3、Caspase-9 mRNA相对表达量比较差异有统计学意义（F=4.328、5.473、4.198，P<0.05）；观察组细胞中LncRNA PCGEM1及Caspase-3、Caspase-9 mRNA相对表达量均显著低于空白对照组和阴性对照组（P<0.05），空白对照组与阴性对照组细胞中LncRNA PCGEM1及Caspase-3、Caspase-9 mRNA相对表达量比较差异无统计学意义（P>0.05）。转染24、48、72、96 h时，3组细胞增殖能力比较差异有统计学意义（F=4.126、4.572、5.218、5.379，P<0.05）；观察组细胞增殖能力低于空白对照组和阴性对照组（P<0.05），空白对照组与阴性对照组细胞增殖能力比较差异无统计学意义（P>0.05）。3组细胞迁移细胞数、细胞凋亡率比较差异有统计学意义（F=6.873、6.844，P<0.05），观察组迁移细胞数及细胞凋亡率显著低于空白对照组和阴性对照组（P<0.05）；空白对照组与阴性对照组迁移细胞数、细胞凋亡率比较差异无统计学意义（P>0.05）。结论　肝癌Huh7细胞中存在LncRNA PCGEM1表达，下调LncRNA PCGEM1的表达能抑制肝癌细胞增殖、侵袭及凋亡。
Abstract:
Objective　To investigate the effects of long noncoding RNA（LncRNA） PCGEM1 on the proliferation，invasion and apoptosis of hepatoma carcinoma cells.Methods　LncRNA PCGEM1 lentivirus silencing expression vector was constructed，and human hepatoma carcinoma Huh7 cells were cultured.The Huh7 cells which in logarithmin growth phase were divided into blank control group，negative control group and observation group.The Huh7 cells in the blank control group were not transfected，while the Huh7 cells in the negative control group were transfected with empty lentivirus vector，the Huh7 cells in the observation group were transfected with LncRNA PCGEM1 lentivirus silencing expression vector.The expression of LncRNA PCGEM1 and Caspase-3，Caspase-9 mRNA in Huh7 cells was detected by real-time fluorescence quantitative polymerase chain reaction.The proliferation ability of Huh7 cells was detected by cell counting kit-8.The migration ability of Huh7 cells was detected by Transwell assay.The apoptosis of Huh7 cells was detected by flow cytometry.Results　There were significant differences in the relative expression of LncRNA PCGEM1 and Caspase-3,Caspase-9 mRNA in Huh7 cells among the three groups （F=4.328，5.473，4.198；P<0.05）.The relative expression of LncRNA PCGEM1 and Caspase-3,Caspase-9 mRNA in Huh7 cells in the observation group was significantly lower than that in the blank control group and the negative control group （P<0.05），but there was no significant difference in the relative expression of LncRNA PCGEM1 and Caspase-3,Caspase-9 mRNA in Huh7 cells between the blank control group and the negative control group （P>0.05）.There were significant differences in the proliferation ability of Huh7 cells among the three groups at 24，48，72 and 96 hours after transfection （F=4.126，4.572，5.218，5.379；P<0.05）；the proliferation ability of Huh7 cells in the observation group was lower than that in the blank control group and the negative control group （P<0.05），but there was no significant difference in the proliferation ability of Huh7 cells between the blank control group and the negative control group（P>0.05）.There were significant differences in the number of migrating cells and apoptotic rate among the three groups （F=6.873，6.844；P<0.05）；the number of migrating cells and apoptotic rate in the observation group were significantly lower than those in the blank control group and the negative control group （P<0.05）；but there was no significant difference in the number of migrating cells and apoptotic rate between the blank control group and the negative control group （P>0.05）.Conclusion　LncRNA PCGEM1 is expressed in hepatoma carcinoma Hub7 cells，and down-regulation of LncRNA PCGEM1 can inhibit the proliferation，invasion and apoptosis of hepatoma carcinoma cells.

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更新日期/Last Update: 2019-05-05