[1]赵　欣,裴科阳,谭　军.腺苷预处理对大鼠局灶性脑缺血再灌注后线粒体活性氧含量及线粒体呼吸链复合体Ⅰ、Ⅲ活性和脑组织总抗氧化能力的影响[J].新乡医学院学报,2019,36(4):305-308.[doi:10.7683/xxyxyxb.2019.04.002]
　ZHAO Xin,PEI Ke-yang,TAN Jun.Effect of adenosine preconditioning on reactive oxygen species content，activity of respiratory chain complex Ⅰ and Ⅲ of mitochondria and the total antioxidant capacity of brain tissueafter focal cerebral ischemia-reperfusion in rats[J].Journal of Xinxiang Medical University,2019,36(4):305-308.[doi:10.7683/xxyxyxb.2019.04.002]
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腺苷预处理对大鼠局灶性脑缺血再灌注后线粒体活性氧含量及线粒体呼吸链复合体Ⅰ、Ⅲ活性和脑组织总抗氧化能力的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
36
期数:
2019年4
页码:
305-308
栏目:
基础研究
出版日期:
2019-04-05

文章信息/Info

Title:
Effect of adenosine preconditioning on reactive oxygen species content，activity of respiratory chain complex Ⅰ and Ⅲ of mitochondria and the total antioxidant capacity of brain tissueafter focal cerebral ischemia-reperfusion in rats
作者:
赵　欣1; 裴科阳1; 谭　军2
（1.新乡医学院，河南　新乡　453003；2.新乡医学院第三附属医院神经内二科，河南　新乡　453003）
Author(s):
ZHAO Xin1; PEI Ke-yang1; TAN Jun2
（1.Xinxiang Medical University，Xinxiang 453003，Henan Province，China；2.Department of Neurology，the Third Affiliated Hospital of Xinxiang Medical University，Xinxiang 453003，Henan Province，China）
关键词:
腺苷; 脑缺血再灌注; 活性氧; 线粒体呼吸链复合体; 总抗氧化能力; 大鼠
Keywords:
adenosine; cerebral ischemia-reperfusion; reactive oxygen species; mitochondria respiratory chain complex; total antioxidant capacity; rat
分类号:
R743.3
DOI:
10.7683/xxyxyxb.2019.04.002
文献标志码:
A
摘要:
目的　观察腺苷预处理对大鼠局灶性脑缺血再灌注后线粒体活性氧（ROS）含量及线粒体呼吸链复合体Ⅰ、Ⅲ活性和脑组织总抗氧化能力（TAC）的影响。方法　将18只雄性Sprague Dawley大鼠随机分为假手术组、缺血再灌注组和腺苷预处理组，每组6只。造模前3 d腺苷预处理组大鼠腹腔注射腺苷注射液（1.5 mg·kg-1），每日1次；假手术组和缺血再灌注组大鼠在相同时间点腹腔注射2 mL生理盐水。缺血再灌注组和腺苷预处理组大鼠采用改良线栓法制作大脑中动脉闭塞（MCAO）模型，假手术组大鼠术中只分离血管，不插入线栓。血流阻断2 h后将线栓拔出，形成再灌注。造模后24 h采用化学荧光法检测3组大鼠缺血区脑组织线粒体中ROS水平及线粒体呼吸链复合体Ⅰ、Ⅲ活性和TAC。结果　缺血再灌注组大鼠缺血区脑组织线粒体中ROS水平显著高于假手术组（t=5.122，P=0.000），腺苷预处理组大鼠缺血区脑组织线粒体中ROS水平显著低于缺血再灌注组（t=4.107，P=0.001）。缺血再灌注组大鼠缺血区脑组织线粒体呼吸链复合体Ⅰ、Ⅲ活性显著低于假手术组（t=3.632、3.472，P=0.002、0.003），腺苷预处理组大鼠缺血区脑组织线粒体呼吸链复合体Ⅰ、Ⅲ活性显著高于缺血再灌注组 （t=2.623、2.193，P=0.019、0.045）。3组大鼠缺血区脑组织TAC比较差异均无统计学意义（F=1.755，P=0.207）。结论　腺苷预处理可通过保护线粒体呼吸链复合体Ⅰ、Ⅲ活性而减少MCAO大鼠脑缺血再灌注后线粒体ROS的生成。
Abstract:
Objective　To explore the effect of adenosine preconditioning on reactive oxygen species（ROS） content，activity of respiratory chain complex Ⅰ and Ⅲ of mitochondria and the total antioxidant capacity（TAC） of brain tissue after focal cerebral ischemia-reperfusion in rats.Methods　Eighteen male Sprague-Dawley rats were randomly divided into sham operation group，ischemia-reperfusion group and adenosine preconditioning group，with 6 rats in each group.The rats in adenosine preconditioning group were intraperitoneally injected with adenosine injection（1.5 mg·kg-1） for 3 consecutive days before modeling，the rats in ischemia-reperfusion and sham operation group were intraperitoneally injected with 2 mL saline at the same time point.The middle cerebral artery occlusion（MCAO）model was established by improved suture-occluded method in ischemia-reperfusion group and adenosine preconditioning group，the rats in the sham operation group were only separated the blood vessels during operation，and were not inserted the thread.The thread bolt was pulled out to form reperfusion after blocking the blood flow for 2 hours.At 24 h after modeling，the ROS content，the activity of respiratory chain complexⅠand Ⅲ in mitochondria and the TAC of brain tissue of rats in the three groups were detected by chemical fluorescence method.Results　The ROS content in mitochondria in ischemic brain tissue of rats in ischemia-reperfusion group was significantly higher than that in the sham group （t=5.122，P=0.000）；the ROS content in mitochondria in ischemic brain tissue of rats in adenosine preconditioning group was significantly lower than that in the ischemia-reperfusion group （t=4.107，P=0.001）.The activity of respiratory chain complexⅠand Ⅲ in mitochondria in ischemic brain tissue of rats in ischemia-reperfusion group was significantly lower than that in the sham group （t=3.632，3.472；P=0.002，0.003）；the activity of respiratory chain complex Ⅰand Ⅲ in mitochondria in ischemic brain tissue of rats in adenosine preconditioning group was significantly higher than that in the ischemia-reperfusion group（t=2.623，2.193；P=0.019，0.045）.There was no significant difference in the TAC in mitochondria in ischemic brain tissue of rats among the three groups（F=1.755，P=0.207）.Conclusion　Adenosine pretreatment can reduce the ROS content in mitochondria by protecting the activity of respiratory chain complex Ⅰ and Ⅲ after ischemia-reperfusion in MCAO rats.

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更新日期/Last Update: 2019-04-05