[1]王文杰,左　霞,郭俐宏,等.大株红景天注射液对大鼠脑缺血再灌注后血管内皮损伤的影响[J].新乡医学院学报,2016,33(1):026-29.[doi:10.7683/xxyxyxb.2016.01.008]
　WANG Wen-Jie,ZUO Xia,GUO Li-hong,et al.Effect of rhodiola kirilowii Regel injection on rats with vascular endothelial induced by cerebral ischemia reperfusion[J].Journal of Xinxiang Medical University,2016,33(1):026-29.[doi:10.7683/xxyxyxb.2016.01.008]
点击复制

大株红景天注射液对大鼠脑缺血再灌注后血管内皮损伤的影响
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年1
页码:
026-29
栏目:
基础研究
出版日期:
2016-01-05

文章信息/Info

Title:
Effect of rhodiola kirilowii Regel injection on rats with vascular endothelial induced by cerebral ischemia reperfusion
作者:
王文杰1; 左　霞1; 郭俐宏1; 彭吉霞2; 刘兴林3; 安庆宝3
（1.十堰市太和医院护理部，湖北　十堰　442000；2.湖北医药学院机能实验室，湖北　十堰　442000；3.湖北医药学院实验动物中心，湖北　十堰　442000）
Author(s):
WANG Wen-Jie1; ZUO Xia1; GUO Li-hong1; PENG Ji-xia2; LIU Xing-lin3; AN Qing-bao3
(1.Department of Nursing，Taihe Hospital of Shiyan City，Shiyan 442000，Hubei Province，China；2.Function Laboratory，Hubei University of Medicine，Shiyan 442000，Hubei Province，China；3.Laboratory Animals Center，Hubei University of Medicine，Shiyan 442000，Hubei Province，China)
关键词:
大鼠; 脑缺血再灌注; 大株红景天注射液; 血管内皮生长因子; 脑缺血梗死百分比
Keywords:
rat; cerebral ischemia reperfusion; Rhodiola kirilowii Regel injection; vascular endothelial growth factor; cerebral ischemia infarction percentage
分类号:
R743.3
DOI:
10.7683/xxyxyxb.2016.01.008
文献标志码:
A
摘要:
目的　观察大株红景天注射液对大鼠脑缺血再灌注损伤后血管内皮损伤的影响，并探讨其作用机制。方法　Sprague Dawley大鼠80只，其中24只未造模型大鼠作为对照组；另56只大鼠采用线栓阻塞大脑中动脉法建立脑缺血再灌注损伤模型，术后选择其中造模成功的48只大鼠随机分为模型组和观察组，每组24只。观察组大鼠经尾静脉注射大株红景天注射液2 mL·kg-1，每日1次；对照组及模型组大鼠经尾静脉注射等量生理盐水。14 d后断头处死各组大鼠，每组取8只大鼠采用酶联免疫吸附试验检测脑组织血管内皮生长因子（VEGF）、受体胎肝激酶-1(FLK-1)、缺氧诱导因子-1α(HIF-1α)蛋白，另取8只大鼠采用反转录聚合酶链反应测定其脑组织VEGF mRNA、FLK-1 mRNA、 HIF-1α mRNA表达，余下8只大鼠采用氯化三苯基四氮唑染色法测定脑缺血梗死区比例。结果　对照组、模型组、观察组大鼠全脑质量比较差异无统计学意义（P>0.05）。对照组大鼠脑组织无缺血梗死；模型组、观察组大鼠的脑缺血梗死区质量及梗死比例均显著高于对照组（P<0.05）；观察组大鼠脑缺血梗死区质量及梗死比例均显著低于模型组（P<0.05）。模型组和观察组大鼠脑组织VEGF、FLK-1、HIF-1α蛋白及其mRNA表达与对照组比较均显著增高（P<0.05）；观察组大鼠脑组织VEGF、FLK-1、 HIF-1α蛋白及其mRNA表达与模型组比较均显著增高（P<0.05）。结论　大株红景天注射液可以升高大鼠缺血脑组织VEGF、FLK-1、 HIF-1α蛋白及其mRNA表达，有助于诱导缺血部位血管内皮分化与再生，促进脑缺血区动脉侧支循环重建，维持成熟血管的完整性，增加脑组织供血，对大鼠脑缺血再灌注损伤具有一定的保护作用。
Abstract:
Objective　To investigate the effect of rhodiola kirilowii Regel injection on rats with vascular endothelial induced by cerebral ischemia reperfusion and to explore the mechanism of action.Methods　Among eighty Sprague Dawley rats，24 rats were not given any operation as the control group，the other 56 rats were performed with occlusion of middle cerebral artery to establish the cerebral ischemia reperfusion models.Forty-eight rats which were built model sucessfully were divided into model group and observation group randomly，with 24 rats in each group.The rats in observation group were given 2 mL·kg-1 rhodiola kirilowii Regel by caudal intravenous injection，once a day.While the rats in control group and model group were given the same volume of physiological saline.The rats were sacrificed after 14 days.The protein expressions of vascular endothelial growth factor (VEGF)，fetal liver receptor kinase 1 (FLK1) and hypoxia inducing factor-1α (HIF-1α) in brain tissues of 8 rats in each group were detected by enzyme-linked immunosorbent assay.The expressions of VEGF mRNA,FLK-1 mRNA and HIF-1α mRNA of another 8 rats in each group were detected by reverse transcription polymerase chain reaction.Cerebral ischemia infarction area of the left 8 rats was evaluated by triphenyltetrazolium chloride staining method.Results　There was no significant difference in total brain mass among the control group，the model group and the observation group (P>0.05).Ischemia and infarction in brain was not found in the control group.Infarct mass and infarct percentage in brain of model group and observation group were significantly higher than those of control group (P<0.05).Infarct mass and infarct percentage in brain of observation group were significantly lower than those of model group (P<0.05).The protein and mRNA expressions of VEGF，FLK-1 and HIF-1α in model group and observation group were significantly higher than those in the control group (P<0.05).The protein and mRNA expressions of VEGF，FLK-1 and HIF-1α in observation group were significantly higher than those in the model group (P<0.05).Conclusion　Rhodiola kirilowii Regel injection plays a role of the protective effect for cerebral ischemia reperfusion in rats by promoting the protein and mRNA expressions of VEGF，FLK-1 and HIF-1α，improving the differentiation and regeneration of blood vessel endothelium in ischemia area，promoting the realignment of arterial collateral circulation in cerebral ischemia area，maintaining the integrality of blood vessel and increasing the blood-supply of brain tissue.

参考文献/References:

［1］　曹俊岭，薛春苗，王艳梅，等.红景天颗粒体内外抗氧化作用研究［J］.中国药学杂志，2014，49（14）：1222-1223.
［2］　杨婷媛，李三鸣，李红菊，等.红景天注射液中红景天苷和酪醇的定性定量分析［J］.中成药，2007，29(12)：1789-1791.
［3］　郎云峰，孟可，赵珩，等.红景天苷对糖尿病模型大鼠海马CA1区ChAT和BDNF阳性神经元的影响［J］.吉林医学，2014，35（19）：4133-4135.
［4］　徐艳炜，孙莉，梁浩，等.脑缺血中12/15-脂氧合酶对过氧化物酶体增殖物激活受体γ调节作用的初步探讨［J］.中华神经科杂志，2014，47(2)：123-127.
［5］　OZMEN S，AYHAN S，DEMIR Y，et al.Impact of gradual blood flow increase on ischaemia-reperfusion injury in the rat cremaster microcirculation model ［J］.J Plast Reconstr Aesthet Surg，2008，61(8)：939-948.
［6］　罗利俊，陈玲，笱玉兰，等.中药联合辛伐他汀治疗急性脑梗死的临床疗效 ［J］.新乡医学院学报，2014，31（9）：747-750.
［7］　王婷，闫咏梅，曹影，等.丹红注射液治疗中风(恢复期)瘀血闭阻证的临床研究［J］.中西医结合心脑血管病杂志，2014，12（2）：148-149.
［8］　王晓娟.丹参多酚酸盐对大鼠骨髓间充质干细胞增殖及血管内皮生长因子表达的影响［J］.环球中医药杂志，2013，6(7)：492-495.
［9］　张莉，刘海燕.通心络胶囊联合美托洛尔治疗冠心病心力衰竭92例［J］.国际中医中药杂志，2014，36（1）：57-58.
［10］　李剑，范维琥，罗心平，等.预灌红景天对心梗大鼠心功能及Flk-1mRNA表达的影响［J］.复旦学报（医学版），2006，33（3）：384-385.
［11］　刘林，薛文，钱耀文，等.缺氧诱导因子-1在大鼠脊髓缺血再灌注损伤中的表达［J］.中华神经外科疾病研究杂志，2013，12（1）：16-18.

相似文献/References:

[1]刘燕青,彭瑞云,高亚兵,等.成年大鼠窦房结的定位方法及其在超微结构研究中的应用[J].新乡医学院学报,,():000.
[2]许春雷,杨廷桐,李东亮,等.鼠大脑局部缺血再灌注的病理学观察[J].新乡医学院学报,1994,11(02):130.
[3]冯广友,杨文亮,常晓岚,等.大鼠间脑向腰骶髓的直接投射[J].新乡医学院学报,1995,12(02):099.
[4]许予明,方岩,赵德华,等.突触的可塑性研究──大鼠运动神经末梢增龄变化[J].新乡医学院学报,1995,12(03):205.
[5]常全忠,张淑玲,周素风,等.急性局部脑缺血─再灌注对大鼠外周血糖的影响[J].新乡医学院学报,1997,14(03):255.
[6]王建芬,周素凤,李东亮,等.大鼠左侧MCA阻塞后运动和行为的观察[J].新乡医学院学报,1997,14(04):346.
[7]王　省　任同明　王庆志.大鼠边缘系统神经肽Y免疫阳性神经元的分布[J].新乡医学院学报,1999,16(02):116.
[8]李艳萍　唐军民　唐　岩.MNNG所致大鼠胃粘膜癌变过程中肾上腺皮质组化研究[J].新乡医学院学报,1999,16(04):298.
[9]李万里　王　萍　王守英　田玉慧　刘晓丽.钙与鸡冠花提取物对氟中毒大鼠骨代谢的影响[J].新乡医学院学报,1999,16(04):289.
[10]刘巨源　陈永凤　郭　萍　高新平　路成吉.黄芪丹参甘草等中药对大鼠肺纤维化的影响[J].新乡医学院学报,1999,16(04):292.
[11]赵红岗 李东亮,王东霞 祈英杰,周素凤,等.黄体酮对大鼠脑缺血区水和钠钾钙含量的影响[J].新乡医学院学报,2000,17(02):077.
[12]赵　欣,裴科阳,谭　军.腺苷预处理对大鼠局灶性脑缺血再灌注后线粒体活性氧含量及线粒体呼吸链复合体Ⅰ、Ⅲ活性和脑组织总抗氧化能力的影响[J].新乡医学院学报,2019,36(4):305.[doi:10.7683/xxyxyxb.2019.04.002]
　ZHAO Xin,PEI Ke-yang,TAN Jun.Effect of adenosine preconditioning on reactive oxygen species content，activity of respiratory chain complex Ⅰ and Ⅲ of mitochondria and the total antioxidant capacity of brain tissueafter focal cerebral ischemia-reperfusion in rats[J].Journal of Xinxiang Medical University,2019,36(1):305.[doi:10.7683/xxyxyxb.2019.04.002]

更新日期/Last Update: 2016-01-05