[1]董　娇,冀紫阳,谷景阳,等.度洛西汀对抑郁症大鼠行为及海马和前额叶皮质中S100B基因甲基化的影响[J].新乡医学院学报,2018,35(4):255-259.[doi:10.7683/xxyxyxb.2018.04.001]
　DONG Jiao,JI Zi-yang,GU Jing-yang,et al.Effect of duloxetine on the behavior and methylation of S100B gene in hippocampus and prefrontal cortex of depression rats[J].Journal of Xinxiang Medical University,2018,35(4):255-259.[doi:10.7683/xxyxyxb.2018.04.001]
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度洛西汀对抑郁症大鼠行为及海马和前额叶皮质中S100B基因甲基化的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
35
期数:
2018年4
页码:
255-259
栏目:
基础研究
出版日期:
2018-04-05

文章信息/Info

Title:
Effect of duloxetine on the behavior and methylation of S100B gene in hippocampus and prefrontal cortex of depression rats
作者:
董　娇; 冀紫阳; 谷景阳; 邵秋静; 王长虹
（新乡医学院第二附属医院临床精神卫生学教研室，河南　新乡　453002）
Author(s):
DONG Jiao; JI Zi-yang; GU Jing-yang; SHAO Qiu-jing; WANG Chang-hong
（Department of Clinical Mental Health，the Second Affiliated Hospital of Xinxiang Medical University，Xinxiang 453002，Henan Province，China）
关键词:
抑郁症; 度洛西汀; 海马; 前额叶皮质; S100B基因; 甲基化
Keywords:
depression; duloxetine; hippocampus; prefrontal cortex; S100B gene; methylation
分类号:
R749.4
DOI:
10.7683/xxyxyxb.2018.04.001
文献标志码:
A
摘要:
目的　探讨度洛西汀对抑郁症大鼠行为及海马和前额叶皮质中S100B基因甲基化的影响。方法　30只成年雄性Sprague-Dawle大鼠按随机数字表法分为对照组、抑郁症组和度洛西汀组，每组10只。抑郁症组和度洛西汀组大鼠于实验第1～42天给予慢性不可预见性刺激（CUMS）制备抑郁症模型，度洛西汀组大鼠于实验第22～42天给予度洛西汀灌胃（7.5 mg·kg-1·d-1）。对照组大鼠不给予CUMS及任何干预措施。各组大鼠分别于造模前（实验前）、造模后（实验第21天）及药物干预后（实验第42天）进行旷场实验、避暗实验，评估3组大鼠的行为学变化；最后1次行为学评估后，处死大鼠取脑组织，采用甲基化特异性聚合酶链反应检测大鼠海马和前额叶皮质中S100B蛋白编码基因启动子区CpG岛甲基化状态。结果　造模前3组大鼠行为学指标比较差异均无统计学意义（P>0.05）。对照组大鼠造模前、造模后及干预后旷场实验中水平运动评分、垂直运动评分及避暗实验中潜伏期比较差异均无统计学意义（P>0.05）。与造模前比较，造模后抑郁症组、度洛西汀组大鼠旷场实验中水平运动评分、垂直运动评分减少（P<0.05），避暗实验中潜伏期延长（P<0.05）。造模后抑郁症组与度洛西汀组大鼠行为学指标比较差异均无统计学意义（P>0.05）。与对照组比较，造模后抑郁症组、度洛西汀组大鼠体质量降低，旷场实验中水平运动评分、垂直运动评分减少（P<0.05），避暗实验中潜伏期延长（P<0.05）。与造模后比较，干预后度洛西汀组大鼠旷场实验中水平运动评分、垂直运动评分增加（P<0.05），潜伏期缩短（P<0.05）。与抑郁症组比较，干预后度洛西汀组大鼠体质量及旷场实验中水平运动评分、垂直运动评分增加（P<0.05），潜伏期缩短（P<0.05）。3组大鼠海马及前额叶皮质中均未呈现S100B蛋白编码基因启动子区CpG岛甲基化状态。结论　度洛西汀可以显著改善抑郁症模型大鼠的行为学表现，其机制可能与海马及前额叶皮层中S100B基因的甲基化状态无关。
Abstract:
Objective　To investigate the effect of duloxetine on the behavior and methylation of S100B gene in hippocampus and prefrontal cortex of depression rats.Methods　Thirty adult male Sprague-Dawle rats were divided into control group，depression group and duloxetine group by random number table method，with ten rats in each group.The rats in the depression group and duloxetine group were given the chronic unpredictable mild stress（CUMS） for 1-42 days to prepare the depression model.The rats in the duloxetine group were given duloxetine （7.5 mg·kg-1·d-1） by intragastric administration from the twenty-second to forty-second days.The rats in the control group did not give CUMS and any intervention.The behavioral changes of rats in each group were evaluated by open field test and dark avoidance test at the time points of before modeling（before experiment），after modeling （the twenty-first day） and after drug intervention（the forty-second day）.After the last behavioral assessment，the rats were killed and the brain tissues were collected.The methylation status of CpG island in the promoter region of the S100B protein encoding gene in the hippocampus and prefrontal cortex of rats was detected by methylation specific polymerase chain reaction.Results　There was no significant difference in the behavioral indexes of rats in the three groups before modeling （P>0.05）.There was no significant difference in the horizontal movement score and vertical movement score of rats in open field test，and the latency in dark avoidance test before modeling，after modeling and after intervention in the control group （P>0.05）.Compared with before modeling，the horizontal movement score and vertical movement score of rats reduced in open field test（P<0.05），and the latency prolonged in dark avoidance test in the depression group and duloxetine group after modeling （P<0.05）.There was no significant difference in the behavioral indexes of rats between the depression group and duloxetine group after modeling （P>0.05）.Compared with the control group，the body mass of rats decreased，the horizontal movement score and vertical movement score of rats reduced in open field test（P<0.05），and the latency prolonged in dark avoidance test in the depression group and duloxetine group after modeling （P<0.05）.Compared with after modeling，the body mass of rats decreased，the horizontal movement score and vertical movement score of rats increased in open field test（P<0.05），and the latency shortened in dark avoidance test in the duloxetine group after intervention （P<0.05）.Compared with the depression group，the horizontal movement score and vertical movement score of rats increased in open field test（P<0.05），and the latency shortened in dark avoidance test in the duloxetine group after intervention （P<0.05）.The methylation status of CpG island in the promoter region of S100B protein gene was not present in the hippocampus and prefrontal cortex of rats in the three groups.Conclusion　Duloxetine can significantly improve the behavioral performance of depression rats，which may not be related to the methylation status of S100B gene in hippocampus and prefrontal cortex.

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更新日期/Last Update: 2018-04-05