[1]刘　勇,徐天娇,霍金莲.Ku70乙酰化在硒诱导人肺腺癌A549细胞凋亡中的作用[J].新乡医学院学报,2017,34(2):099-103.[doi:10.7683/xxyxyxb.2017.02.005]
　LIU Yong,XU Tian-jiao,HUO Jin-lian.Effect of Ku70 acetylation on selenium-induced apoptosis of human pulmonary adenocarcinoma A549 cell[J].Journal of Xinxiang Medical University,2017,34(2):099-103.[doi:10.7683/xxyxyxb.2017.02.005]
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Ku70乙酰化在硒诱导人肺腺癌A549细胞凋亡中的作用
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
34
期数:
2017年2
页码:
099-103
栏目:
基础研究
出版日期:
2017-02-05

文章信息/Info

Title:
Effect of Ku70 acetylation on selenium-induced apoptosis of human pulmonary adenocarcinoma A549 cell
作者:
刘　勇1; 徐天娇2; 霍金莲1
（1.延安大学咸阳医院老年病科，陕西　咸阳　712000；2.西安医学院基础医学部药理学研究室，陕西　西安　710021）
Author(s):
LIU Yong1; XU Tian-jiao2; HUO Jin-lian1
(1.Department of Geratology,Xianyang Hospital of Yan′an University,Xianyang 712000,Shaanxi Province,China;2.Department of Pharmacology,College of Basic Medicine of Xi′an Medical University,Xi′an 710021,Shaanxi Province,China)
关键词:
硒; 肺腺癌; A549细胞; Ku70蛋白; 细胞凋亡
Keywords:
selenium; pulmonary adenocarcinoma; A549 cell; Ku70 protein; apoptosis
分类号:
R734.2
DOI:
10.7683/xxyxyxb.2017.02.005
文献标志码:
A
摘要:
目的　探讨Ku70乙酰化在硒诱导人肺腺癌A549细胞凋亡中的作用。方法　将体外培养的人肺腺癌A549细胞分为对照组(用含体积分数10%胎牛血清的达尔伯克改良伊格尔培养基培养）和1、5、10 μmol·L-1硒处理组（分别用含1、5、10 μmol·L-1硒的达尔伯克改良伊格尔培养基培养），分别培养24、48 h，通过Western blot法检测Ku70表达，免疫沉淀法检测乙酰化Ku70和Ku70-Bax、Bax-Ku70的表达。将Ku70 siRNA转染后的人肺腺癌A549细胞分为对照组和1、5、10 μmol·L-1硒处理组，通过Western blot法检测凋亡相关蛋白Bax和Bcl-2的表达。结果　与同一时间点对照组比较，1 μmol·L-1硒处理组Ku70、Bax、Bcl-2、乙酰化Ku70、Ku70-Bax和Bax-Ku70表达差异无统计学意义(P>0.05)；与同一时间点对照组、1 μmol·L-1硒处理组比较，5、10 μmol·L-1硒处理组Ku70、Bcl-2、Ku70-Bax和Bax-Ku70表达均减少（P<0.01)，Bax、乙酰化Ku70表达均增加（P<0.01)；与同一时间点 5 μmol·L-1 硒处理组比较，10 μmol·L-1硒处理组Ku70、Bcl-2、Ku70-Bax和Bax-Ku70表达均减少（P<0.01)，Bax、乙酰化Ku70表达均增加（P<0.01)。与本组24 h时比较，48 h时1 μmol·L-1硒处理组Ku70、Bax、Bcl-2、乙酰化Ku70、Ku70-Bax和Bax-Ku70 表达差异无统计学意义(P>0.05)；与本组24 h时比较，48 h时5、10 μmol·L-1硒处理组Ku70、Bcl-2、Ku70-Bax和Bax-Ku70表达减少（P<0.05)，Bax、乙酰化Ku70表达增加（P<0.05)。结论　硒通过促使Ku70乙酰化诱导Bax依赖性人肺腺癌A549细胞凋亡。
Abstract:
Objective　To investigate the effect of Ku70 acetylation on selenium-induced apoptosis of human pulmonary adenocarcinoma A549 cell.Methods　The A549 cells which cultured in vitro were divided into control group(the cells were cultured in Dulbecco′s modified Eagle′s medium containing 10% fetal bovine serum) and 1,5,10 μmol·L-1 selenium group(the cells were cultured in Dulbecco′s modified Eagle′s medium containing 1,5,10 μmol·L-1 selenium).At the time points of 24,48 h after cultivation,the expression of Ku70 was detected by Western blotting,the acetylizad Ku70,Ku70-Bax and Bax-Ku70 were detected by immuno-precipitation method.The A549 cells which were transfected with Ku70 siRNA were divided into control group and 1,5,10 μmol·L-1 selenium group,the levels of Bax and Bcl-2 protein were detected by Western blotting.Results　There was statistic difference in the level of Ku70,Bax,Bcl-2,acetylated Ku70,Ku70-Bax and Bax-Ku70 between control group and 1 μmol·L-1 selenium group at the same time (P>0.05);Compared with control group and 1 μmol·L-1 selenium group at the same time,5,10 μmol·L-1 selenium could significantly down-regulate the levels of Ku70，Bcl-2,Ku70-Bax and Bax-Ku70(P<0.01),and up-regulate the levels of Bax and acetylated Ku70(P<0.01);Compared with 5 μmol·L-1 selenium group at the same time,10 μmol·L-1 selenium could significantly down-regulate the levels of Ku70，Bcl-2,Ku70-Bax and Bax-Ku70(P<0.01),and up-regulate the levels of Bax and acetylated Ku70(P<0.01).There was no statistic difference in the levels of Ku70,Bax,Bcl-2,acetylated Ku70,Ku70-Bax and Bax-Ku70 at the time points of 24 h and 48 h in 1 μmol·L-1 selenium group(P>0.05).Compared with the time point of 24 h,the levels of Ku70，Bcl-2,Ku70-Bax and Bax-Ku70 decreased significantly（P<0.05),and the levels of Bax and acetylated Ku70 increased significantly in 5,10 μmol·L-1 selenium groups at the time point of 48 h（P<0.05).Conclusion　Selenium can induce the Bax-dependent apoptosis of human pulmonary adenocarcinoma A549 cells by promoting Ku70 acetylation.

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更新日期/Last Update: 2017-02-05