[1]郝明华,徐　萍,李亚娜,等.刺梨黄酮对辐射损伤骨髓细胞周期的影响[J].新乡医学院学报,2016,33(12):1044-1046.[doi:10.7683/xxyxyxb.2016.12.007]
　HAO Ming-hua,XU Ping,LI Ya-na,et al.Effect of flavonoids of rosaroxburghii tratt on the cell cycle of bone marrow cells damaged by radiation[J].Journal of Xinxiang Medical University,2016,33(12):1044-1046.[doi:10.7683/xxyxyxb.2016.12.007]
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刺梨黄酮对辐射损伤骨髓细胞周期的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年12
页码:
1044-1046
栏目:
基础研究
出版日期:
2016-12-05

文章信息/Info

Title:
Effect of flavonoids of rosaroxburghii tratt on the cell cycle of bone marrow cells damaged by radiation
作者:
郝明华; 徐　萍; 李亚娜; 王丽娟
（新乡医学院药学院，河南　新乡　453003）
Author(s):
HAO Ming-hua; XU Ping; LI Ya-na; WANG Li-juan
(Pharmacy College of Xinxiang Medical University，Xinxiang 453003，Henan Province,China)
关键词:
刺梨黄酮; 辐射; 骨髓细胞; 细胞周期
Keywords:
flavonoids of rosaroxburghii tratt; radiation; bone marrow cells; cell cycle
分类号:
Q946
DOI:
10.7683/xxyxyxb.2016.12.007
文献标志码:
A
摘要:
目的　研究刺梨黄酮（FRT）对辐射损伤骨髓细胞周期的影响。方法　取小鼠骨髓细胞制成单细胞悬液，分为空白对照组、辐射组、辐射+ 50 mg·L-1 FRT干预组（50 mg·L-1 FRT干预组）、辐射+100 mg·L-1 FRT干预组（100 mg·L-1 FRT干预组）。空白对照组不加干预；辐射组给予6 Gy γ 射线照射；50、100 mg·L-1 FRT干预组先在骨髓细胞悬液加入相应量的FRT，使FRT终浓度分别为50、100 mg·L-1，2 h后给予6 Gy γ射线照射。经细胞固定、碘化丙啶染色后，进行流式细胞仪检测。结果　与空白对照组比较，辐射组照射后6、12 h，G1期、S期细胞比例均降低（P<0.05），G2期细胞比例均增高（P<0.05），且辐射后12 h G1和S期细胞比例低于6 h时（P<0.05），G2期细胞比例显著高于6 h时（P<0.05）。与辐射组比较，50、100 mg·L-1 FRT干预组的G1、S期细胞比例均增高（P<0.05），G2期细胞比例均减少（P<0.05），且辐射后6、12 h，100 mg·L-1 FRT干预组G1和S期细胞比例高于50 mg·L-1FRT干预组（P<0.05），G2期细胞比例低于50 mg·L-1 FRT干预组（P<0.05）。结论　FRT可以降低辐射后骨髓细胞G2期的细胞比例及增高G1、S期的细胞所占比例，对γ射线所致骨髓细胞损伤有一定防护作用，且在一定浓度范围内防护效果呈浓度依赖性。
Abstract:
Objective　To study the effect of flavonoids of rosaroxburghii tratt(FRT)on the cell cycle of bone marrow cells damaged by radiation.Methods　The mice bone marrow cells were taken and made into single cell suspension which was divided into blank control group，radiation group，radiation+50 mg·L-1FRT intervention group(50 mg·L-1FRT intervention group)，radiation+100 mg·L-1FRT intervention group(100 mg·L-1FRT intervention group).The blank control group was not given any intervention;the radiation group was irradiated with 6 Gy gamma rays;the 50,100 mg·L-1FRT intervention group were given appropriate amount of FRT and made the final concentration of FRT was 50,100 mg·L-1;after 2 hours,the 6 Gy gamma ray was irradiated.After the cells fixing and propidium iodide staining，the samples were analyzed by flow cytometry.Results　Compared with the blank control group,the G1 phase,S phase cell percentage in the radiation group was lower(P<0.05),but the cell percentage of G2 phase was higher(P<0.05)after irradiating for 6,12 h;the cell percentage of G1 and S phase after irradiating for 12 h was lower than 6 h in radiation group,the cell percentage of G2 phase after irradiating for 12 h was significantly higher than 6 h in radiation group(P<0.05).Compared with the radiation group,the cell percentage of G1 phase and S phase in 50,100 mg·L-1FRT intervention group was increased(P<0.05);but the cell percentage of G2 phase was decreased(P<0.05).The cell perentage of G1 and S phase in 100 mg·L-1 FRT invention group was higher than that in 50 mg·L-1 FRT invention group and the cell percentage of G2 phase in 100 mg·L-1FRT intervention group was significanlty lower than that in 50 mg·L-1FRT intervention group after irradiating for 6,12 h(P<0.05).Conclusion　FRT can reduce the cell percentage of bone marrow cells in G2 phase and increase the proportion of G1,S phase cells after radiation.FRT has a certain protective effect on bone marrow cell which damaged by gamma ray,and the protective effect was concentration dependent in a certain range of concentration.

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更新日期/Last Update: 2016-12-05