[1]马斐斐,曹丁丁,李　伟,等.趋化因子CXCL1在肥胖小鼠脂肪组织中的表达及意义[J].新乡医学院学报,2016,33(10):849-851.[doi:10.7683/xxyxyxb.2016.10.003]
　MA Fei-fei,CAO Ding-ding,LI Wei,et al.Expression and significance of chemokine CXCL1 in the adipose tissue of mice with obesity[J].Journal of Xinxiang Medical University,2016,33(10):849-851.[doi:10.7683/xxyxyxb.2016.10.003]
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趋化因子CXCL1在肥胖小鼠脂肪组织中的表达及意义
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年10
页码:
849-851
栏目:
基础研究
出版日期:
2016-10-05

文章信息/Info

Title:
Expression and significance of chemokine CXCL1 in the adipose tissue of mice with obesity
作者:
马斐斐1; 曹丁丁2; 李　伟3; 唐认桥3; 欧阳胜荣1; 吴建新1
（1.首都儿科研究所生化研究室，北京　100020；2.北京大学首都儿科研究所教学医院，北京　100020；3.北京协和医学院研究生院，北京　100730）
Author(s):
MA Fei-fei1; CAO Ding-ding2; LI Wei3; TANG Ren-qiao3; OUYANG Sheng-rong1; WU Jian-xin1
(1.Department of Biochemistry,Capital Institute of Pediatrics,Beijing 100020，China;2.Clinical College,Capital Institute of Pediatrics，Beijing 100020，China;3.Graduate School,Chinese Academy of Medical Science,Beijing 100730，China)
关键词:
肥胖; 脂肪细胞分化; 炎症; 趋化因子
Keywords:
obesity; adipocyte differentiation; inflammation; chemokine
分类号:
R725.8；R723.14
DOI:
10.7683/xxyxyxb.2016.10.003
文献标志码:
A
摘要:
目的　探讨趋化因子CXCL1在肥胖小鼠脂肪组织中的表达及意义。方法　将20只小鼠随机分为高脂饲料喂养组和普通饲料喂养组，每组10只，分别给予高脂饲料和普通饲料喂养。提取2组小鼠附睾脂肪组织总RNA，应用实时荧光定量聚合酶链反应检测小鼠附睾脂肪组织中CXCL1 mRNA表达水平；成脂诱导脂肪间充质干细胞，酶联免疫吸附试验检测细胞培养上清液中CXCL1的表达。结果　与普通饲料喂养组小鼠比较，高脂饲料喂养组小鼠附睾脂肪组织中CXCL1 mRNA表达显著增加（P=0.001）；CXCL1蛋白表达水平随脂肪细胞分化时间延长而逐渐增加。结论　小鼠肥胖状态下CXCL1表达明显增加，推测CXCL1在肥胖的发生发展过程中发挥重要作用。
Abstract:
Objective　To observed the expression and significance of chemokine CXCL1 in the adipose tissue of mice with obesity.Methods　Twenty mice were randomly divided into high fat feeding group and common feeding group,10 mice in each group.The mice in high fat feeding group and common feeding group were fed with high fat diet and common diet respectively.The total RNA was extracted from the epididymal adipose tissue of mice in the two groups.The expression of CXCL1 mRNA in the epididymal adipose tissue of the mice in the two group were detected by real-time fluorescence quantitative polymerase chain reaction.The levels of CXCL1 in the cell culture supernatant was detected by enzyme-linked immunosorbent assay after adipogenic treatment of adipose-derived mesenchymal stem cells.Results　Compared with common feeding group,CXCL1 mRNA expression in epididymal adipose tissue of the mice increased significantly in high fat feeding group（P=0.001）.CXCL1 expression increased gradually with the extending of the adipocyte differentiation time.Conclusion　The expression of CXCL1 is significantly increased in the obese mice,which implys that CXCL1 plays an important role in the development of obesity.

参考文献/References:

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更新日期/Last Update: 2016-10-05