[1]王国戗,杨亚莉,王小方,等.青霉素酶免疫血清对产青霉素酶葡萄球菌耐药性的影响[J].新乡医学院学报,2016,33(8):657-661.[doi:10.7683/xxyxyxb.2016.08.002]
　WANG Guo-qiang,YANG Ya-li,WANG Xiao-fang,et al.Effect of penicillinase immune serum on drug tolerance of producing penicillinase Staphylococcus aureus[J].Journal of Xinxiang Medical University,2016,33(8):657-661.[doi:10.7683/xxyxyxb.2016.08.002]
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青霉素酶免疫血清对产青霉素酶葡萄球菌耐药性的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年8
页码:
657-661
栏目:
基础研究
出版日期:
2016-07-27

文章信息/Info

Title:
Effect of penicillinase immune serum on drug tolerance of producing penicillinase Staphylococcus aureus
作者:
王国戗1; 杨亚莉2; 王小方1; 汪　磊1; 潘亚晶1; 张　磊1; 仲　华1; 张华丽1; 范艳茹1; 武艳伟1; 林志强1; 薛会朝3; 郭长磊4; 齐劲松5; 袁晓梅6
（1.新乡医学院第一附属医院检验科，河南　卫辉　453100；2.新乡市第一人民医院消化科，河南　新乡　453000；3.新乡医学院第一附属医院甲状腺乳腺外科，河南　卫辉　453100；4.新乡医学院第一附属医院心血管内一科，河南　卫辉　453100；5.新乡医学院第一附属医院介入科，河南　卫辉　453100；6.新乡医学院第一附属医院呼吸科，河南　卫辉　453100）
Author(s):
WANG Guo-qiang1; YANG Ya-li2; WANG Xiao-fang1; WANG Lei1; PAN Ya-jing1; ZHANG Lei1; ZHONG Hua1; ZHANG Hua-li1; FAN Yan-ru1; WU Yan-wei1; LIN Zhi-qiang1; XUE Hui-cao3; GUO Chang-lei4; QI Jin-song5; YUAN Xiao-mei6
（1.Clinical Laboratory，First Affiliated Hospital of Xinxiang Medical University；2.Department of Gastroenterology，First People′s Hospital of Xinxiang；3.Department of Thyroid and Breast Surgery，First Affiliated Hospital of Xinxiang Medical University；4.First Department of Cardiology，First Affiliated Hospital of Xinxiang Medical University；5.Department of Interventional Radiology，First Affiliated Hospital of Xinxiang Medical University；6.Department of Pneumology，First Affiliated Hospital of XXMU）
关键词:
青霉素; 青霉素酶; 免疫血清; 金黄色葡萄球菌; 动物感染模型
Keywords:
penicillin; penicillinase; immune serum; Staphylococcus aureus; animal infection model
分类号:
R965
DOI:
10.7683/xxyxyxb.2016.08.002
文献标志码:
A
摘要:
目的　探讨青霉素酶免疫血清在治疗产青霉素酶葡萄球菌感染中的作用。方法　采用青霉素酶加弗氏完全佐剂、弗氏不完全佐剂充分乳化后，于新西兰兔背部皮下多点注射，制备青霉素酶免疫血清。以肉汤培养管中加入产青霉素酶的金黄色葡萄球菌、青霉素酶免疫血清和青霉素为实验组，同时设立肉汤培养管中加入产青霉素酶的金黄色葡萄球菌和青霉素酶免疫血清的实验对照组、仅有营养肉汤的实验空白组和肉汤培养管中加入青霉素和产青霉素酶的金黄色葡萄球菌的空白组，进行体外实验，观察培养基有无细菌生长，并采用Phoenix100细菌鉴定仪鉴定所生长细菌是否为金黄色葡萄球菌。同时制备新西兰兔产青霉素酶金黄色葡萄球菌感染模型，分为实验组（经耳静脉注射青霉素酶免疫血清和青霉素）、空白对照组（仅经耳静脉注射青霉素）、实验对照组（仅经耳静脉注射青霉素酶免疫血清），连续观察各组新西兰兔体温、血培养变化。结果　体外培养72 h后，实验组、实验空白组无细菌生长；空白组和实验对照组有金黄色葡萄球菌生长。体内注射后第2、3、4、5、6天，实验组新西兰兔体温升高者均少于实验对照组（χ2=5.051、5.051、5.051、7.500、7.540，P<0.05）和空白对照组（χ2=7.200、5.058、7.200、5.495、7.540，P<0.05）；第7天，实验组新西兰兔与实验对照组和空白对照组比较，体温增高者差异无统计学意义（χ2=3.058、3.529，P>0.05）。第1、2、3、4、5、6、7天，实验对照组新西兰兔与空白对照组比较体温增高者差异无统计学意义（χ2=0.000、0.267、0.000、0.267、0.220、0.059、0.024，P>0.05）。接种后第2天，实验组新西兰兔血培养阳性者与实验对照组比较差异无统计学意义（χ2=3.333，P>0.05），但显著低于空白对照组（χ2=5.051，P<0.05）；实验对照组新西兰兔血培养阳性者与空白对照组比较差异无统计学意义（χ2=0.220，P>0.05）。接种后第4天，实验组新西兰兔血培养阳性者显著低于实验对照组和空白对照组（χ2=7.500、7.500，P<0.05），实验对照组新西兰兔血培养阳性者与空白对照组比较差异无统计学意义（χ2=0.000，P>0.05）。接种后第6天，实验组新西兰兔血培养阳性者显著低于实验对照组和空白对照组（χ2=7.540、5.000，P<0.05）；实验对照组与空白对照组比较差异无统计学意义（χ2=0.460，P>0.05）。结论　青霉素酶免疫血清可以消除金黄色葡萄球菌因产青霉素酶而产生的对青霉素类抗生素的耐药作用。
Abstract:
Objective　To investigate the function of penicillinase immune serum in the treatment of producing penicillinase staphylococci infection.Methods　Penicillinase was emulsionized by Freund′s complete adjuvant and Freund′s incomplete adjuvant，the admixture was multi-point subcutaneous injected in New Zealand rabbit to prepare the penicillinase immune serum.Experimental group（added the producing penicillinase Staphylococcus aureus and penicillin and penicillinase immune serum in the broth tube） and experimental control group（add the producing penicillinase Staphylococcus aureus and penicillinase immune serum in the broth tube）and experimental blank group（only broth in tube）and blank group（add the producing penicillinase Staphylococcus aureus and penicillin in the broth tube） were set up to observe if having bacteria growth in the above groups and identifying the growing bacteria to be or not Staphylococcus aureus by Phoenix100.New Zealand rabbits infection model was prepared by using producing penicillinase Staphylococcus aureus，experimental group（via ear vein injection of penicillinase immune serum and penicillin） and blank control group（only the ear vein injection of penicillin），experimental control group（only the ear vein injection of penicillinase immune serum） were set up and New Zealand rabbits body temperature and blood culture change were continuous observed.Results　After cultured 72 h in vitro，experimental group and experimental blank group had no bacterial growth；blank group and experimental control group grew Staphylococcus aureus.The second to the sixth day after injection，the number of fervescence in experimental group was less than that in the experimental control group（χ2=5.051，5.051，5.051，7.500，7.540，P<0.05） and blank control group（χ2=7.200，5.058，7.200，5.495，7.540，P<0.05）；on the seventh day，compared with experimental control group and blank control group，there was no significant difference in the number of fervescence in the experimental group（χ2=3.058，3.529，P>0.05）.On the first to the seventh day，there was no significant difference in the number of fervescence between the experimental control group and the blank control group（χ2=0.000，0.267，0.000，0.267，0.220，0.059，0.024，P>0.05）.On the second day after inoculation，the blood culture positive had no statistically significant difference between the experimental group and the experimental control group（χ2=3.333，P>0.05），but significantly lower than the blank control group（χ2=5.051，P<0.05）；the blood culture positive had no statistically significant difference between the experimental control group and the blank control group（χ2=0.220，P>0.05）.On the fourth days after inoculation，the blood culture positive rate in the experimental group was significantly lower than that in the experimental control group and blank control group（χ2=7.500，7.500，P<0.05），there was no significant difference in the blood culture positive rate between the experimental control group and blank control group（χ2=0.000，P>0.05）.On the sixth day after inoculation，the blood culture positive rate in the experimental group was significantly lower than in the experimental control group and blank control group（χ2=7.540，5.000，P<0.05）；there was no significant difference in the blood culture positive rate between the experimental control group and blank control group（χ2=0.460，P>0.05）.Conclusion　The penicillinase immune serum can be used to eliminated the drug resistance of producing penicillinase Staphylococcus aureus to penicillin，which due to Staphylococcus aureus producing penicillinase.

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更新日期/Last Update: 2016-08-05