[1]秦胜利.原花青素对大鼠缺血再灌注损伤脑组织中超氧化物歧化酶、谷胱甘肽过氧化物酶、丙二醛和过氧化脂质水平的影响[J].新乡医学院学报,2016,33(8):662-663.[doi:10.7683/xxyxyxb.2016.08.003]
　QIN Sheng-li.Effect of proanthocyanidin on the levels of superoxide dismutase，glutathione peroxidase，malondialdehyde and lipid peroxides in brain tissue of rats with cerebral ischemia reperfusion injury[J].Journal of Xinxiang Medical University,2016,33(8):662-663.[doi:10.7683/xxyxyxb.2016.08.003]
点击复制

原花青素对大鼠缺血再灌注损伤脑组织中超氧化物歧化酶、谷胱甘肽过氧化物酶、丙二醛和过氧化脂质水平的影响
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年8
页码:
662-663
栏目:
基础研究
出版日期:
2016-07-27

文章信息/Info

Title:
Effect of proanthocyanidin on the levels of superoxide dismutase，glutathione peroxidase，malondialdehyde and lipid peroxides in brain tissue of rats with cerebral ischemia reperfusion injury
作者:
秦胜利
（焦作市中站区人民医院药剂科，河南　焦作　454191）
Author(s):
QIN Sheng-li
（Department of Pharmacy，the People′s Hospital of Zhongzhan District of Jiaozuo City，Jiaozuo 454191，Henan Province，China）
关键词:
原花青素; 超氧化物歧化酶; 谷胱甘肽过氧化物酶; 丙二醛; 过氧化脂质; 大鼠; 脑; 缺血再灌注损伤
Keywords:
proanthocyanidin; superoxide dismutase; glutathione peroxidase; malondialdehyde; lipid peroxides; cerebral ischemia-reperfusion injury
分类号:
R965
DOI:
10.7683/xxyxyxb.2016.08.003
文献标志码:
A
摘要:
目的　探讨原花青素对大鼠缺血再灌注损伤脑组织中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）和过氧化脂质（LPO）水平的影响。方法　64只健康雄性Sprague-Dawley大鼠随机分为假手术组、模型组、原花青素高剂量（160 mg·kg-1）组和原花青素低剂量（80 mg·kg-1）组，每组16只。采用改良的Zea-Longa线栓法制备大鼠局灶性脑缺血再灌注损伤模型，并对每组大鼠进行相应的干预处理；比较4组大鼠脑组织中SOD、GSH-Px、MDA和LPO水平。结果　与假手术组比较，模型组大鼠脑组织中SOD、GSH-Px水平显著降低（P<0.05），而MDA、LPO水平显著升高（P<0.05）。与模型组比较，原花青素高、低剂量组大鼠脑组织中SOD、GSH-Px水平显著升高（P<0.05），而MDA、LPO水平显著降低（P<0.05）。原花青素高剂量组大鼠脑组织中SOD、GSH-Px水平显著高于原花青素低剂量组（P<0.05），而MDA、LPO水平显著低于原花青素低剂量组（P<0.05）。结论　原花青素可能通过其抗氧化作用对大鼠脑组织缺血再灌注损伤起到保护作用。
Abstract:
Objective　To investigate the effect of proanthocyanidin on the levels of superoxide dismutase（SOD），glutathione peroxidase （GSH-Px），malondialdehyde （MDA） and lipid peroxides （LPO） in brain tissue of rats with cerebral ischemia reperfusion injury.Methods　Sixty-four healthy male Sprague-Dawley rats were randomly divided into sham operation group，model group，high-dose proanthocyanidin group（160 mg·kg-1） and low-dose proanthocyanidin group （80 mg·kg-1），16 rats in each group.The models of focal cerebral ischemia reperfusion injury in rats were established by modified Zea-Longa suture method.The rats in the four groups were treated with corresponding intervention.The levels of SOD，GSH-Px，MDA and LPO in brain tissue of rats were detected in the four groups.Results　The SOD and GSH-Px levels in brain tissue of rats in model group were significantly lower than those in sham operation group （P<0.05），but the MDA and LPO levels in brain tissue of rats in model group were significantly higher than those in sham operation group （P<0.05）.The SOD and GSH-Px levels in brain tissue of rats in high- and low-dose proanthocyanidin group were significantly higher than those in model group（P<0.05），but the MDA and LPO levels in brain tissue of rats in high- and low-dose proanthocyanidin group were significantly lower than those in model group（P<0.05）.The SOD and GSH-Px levels in brain tissue of rats in high-dose proanthocyanidin group were significantly higher than those in low-dose proanthocyanidin group（P<0.05），but the MDA and LPO levels in brain tissue of rats in high-dose proanthocyanidin group were significantly lower than those in low-dose proanthocyanidin group（P<0.05）.Conclusion　The antioxidant effect of proanthocyanidin may play a protective role in cerebral ischemia reperfusion injury in rats.

参考文献/References:

［1］　WHITE B C，GROSSMAN L I，O′NEIL B J，et al.Global brain ischemia and reperfusion［J］.Ann Emerg Med，1996，27（5）：588-594.
［2］　SANDERSON T H，REYNOLDS C A，KUMAR R，et al.Molecular mechanisms of ischemia-reperfusion injury in brain：pivotal role of the mitochondrial membrane potential in reactive oxygen species generation［J］.Mol Neurobiol，2013，47（1）：9-23.
［3］　高璐，王滢，饶胜其，等.葡萄籽原花青素提取物对衰老模型小鼠抗氧化作用［J］.食品科学，2014，35（23）：253-256.
［4］　苏建华，陈玉芳，丁新生，等.甲钴胺对脑缺血再灌注损伤大鼠细胞凋亡及细胞色素C表达的影响 ［J］.中华老年心脑血管病杂志，2015，17（11）：1193-1196.
［5］　张妤，陈娟，韩艳艳，等.腺苷预处理对局灶性脑缺血再灌注损伤大鼠脑组织中低氧诱导因子-1α表达的影响［J］.新乡医学院学报，2014，31（4）：272-274，277.
［6］　KALOGERIS T，BAO Y，KORTHUIS R J.Mitochondrial reactive oxygen species：a double edged sword in ischemia/reperfusion vs preconditioning［J］.Redox Biol，2014，2：702-714.
［7］　吕静，姜慧萍，王腾飞，等.脑缺血再灌注损伤主要发病机制的研究进展［J］.滨州医学院学报，2015，38（4）：291-293.
［8］　黄晓辉，茹晶晶，罗五魁，等.葡萄籽中原花青素的抗氧化性能的研究［J］.宁德师范学院学报（自然科学版），2015，27（2）：136-139.
［9］　郭俊国，赵晴，王兴荣，等.六锐胶囊对葡萄膜炎大鼠血清SOD、MDA和NT-3含量的影响［J］.眼科新进展，2016，36（4）：334-335，344.
［10］　邱咏园，唐映红，王蓓，等.黄芪和三七4种有效成分配伍对小鼠脑缺血再灌注早期抗氧化应激物质活性的影响［J］.中华中医药杂志，2014，29（6）：1940-1943.
［11］　赵林静，白瑞樱，陈正跃，等.脂联素后处理对3周龄大鼠心肌缺血再灌注损伤的保护作用［J］.中华实用儿科临床杂志，2015，30（9）：718-720.

相似文献/References:

[1]李彩莲,王小引,崔泰震.超氧化物歧化酶对缺氧耐受小鼠脑兴奋性氨基酸含量的影响[J].新乡医学院学报,2002,19(02):094.
[2]李彩莲,王小引,崔泰震.超氧化物歧化酶对缺氧耐受小鼠脑兴奋性氨基酸含量的影响[J].新乡医学院学报,2002,19(02):094.
[3]贺玲.原花青素对大鼠视网膜缺血再灌注损伤后视神经的保护作用[J].新乡医学院学报,2012,29(01):000.
[4]李　慧.阿替普酶溶栓后联合丹参多酚酸治疗急性脑梗死的疗效及安全性[J].新乡医学院学报,2019,36(11):1048.[doi:10.7683/xxyxyxb.2019.11.010]
　LI Hui.Efficacy and safety of alteplase combined with danshen polyphenolic acid in treatment of patients with acute cerebral infarction[J].Journal of Xinxiang Medical University,2019,36(8):1048.[doi:10.7683/xxyxyxb.2019.11.010]
[5]项广宇.氯吡格雷联合阿托伐他汀钙治疗短暂性脑缺血发作疗效观察[J].新乡医学院学报,2019,36(1):081.[doi:10.7683/xxyxyxb.2019.01.018]
　XIANG Guang-yu.Effect of clopidogrel combined with atorvastatin calcium in the treatment of transient ischemic attack[J].Journal of Xinxiang Medical University,2019,36(8):081.[doi:10.7683/xxyxyxb.2019.01.018]
[6]杨　勇,魏东明,陈亚武.闭式体外循环下微创主动脉瓣膜置换术治疗主动脉瓣病变疗效观察[J].新乡医学院学报,2021,38(10):970.[doi:10.7683/xxyxyxb.2021.10.015]
　YANG Yong,WEI Dongming,CHEN Yawu.Effect of minimally invasive aortic valve replacement under closed cardiopulmonary bypass in the treatment of aortic valve disease[J].Journal of Xinxiang Medical University,2021,38(8):970.[doi:10.7683/xxyxyxb.2021.10.015]
[7]魏园园,栗延伟,郭　升,等.腺苷激酶抑制剂对大鼠局灶性脑缺血再灌注损伤早期氧化应激和神经元凋亡的影响[J].新乡医学院学报,2020,37(10):921.[doi:10.7683/xxyxyxb.2020.10.005]
　WEI Yuanyuan,LI Yanwei,GUO Sheng,et al.Effect of adenosine kinase inhibitor on oxidative stress and neuronal apoptosis in the early stage of focal cerebral ischemia-reperfusion injury[J].Journal of Xinxiang Medical University,2020,37(8):921.[doi:10.7683/xxyxyxb.2020.10.005]
[8]石计朋,栗延伟,郭丽娟,等.ω-3多不饱和脂肪酸对脂多糖所致脑损伤新生大鼠海马组织中氧化应激产物和细胞凋亡的影响[J].新乡医学院学报,2018,35(6):455.[doi:10.7683/xxyxyxb.2018.06.002]
　SHI Ji-peng,LI Yan-wei,GUO Li-juan,et al.Effect of ω-3 polyunsaturated fatty acid on oxidative stress production and apoptosis in hippocampus of neonatal rats with brain injury induced by lipopolysaccharide[J].Journal of Xinxiang Medical University,2018,35(8):455.[doi:10.7683/xxyxyxb.2018.06.002]
[9]翟慧娟,朱明明.心脑宁对皮肤衰老小鼠的干预性作用[J].新乡医学院学报,2017,34(2):104.[doi:10.7683/xxyxyxb.2017.02.006]
　ZHAI Hui-juan,ZHU Ming-ming.Intervention effect of Xinnaoning on skin aging mice[J].Journal of Xinxiang Medical University,2017,34(8):104.[doi:10.7683/xxyxyxb.2017.02.006]
[10]高　为,张启旺,柯磊磊,等.经尿道输尿管镜碎石术与微创经皮肾镜取石术治疗嵌顿性输尿管上段结石疗效比较[J].新乡医学院学报,2022,39(5):465.[doi:10.7683/xxyxyxb.2022.05.013]
　GAO Wei,ZHANG Qiwang,KE Leilei,et al.Comparison of curative effect between transurethral ureteroscopic lithotripsy and minimally invasive percutaneous nephrolithotomy for incarcerated upper ureteral calculi[J].Journal of Xinxiang Medical University,2022,39(8):465.[doi:10.7683/xxyxyxb.2022.05.013]

更新日期/Last Update: 2016-08-05