[1]任佩佩,樊晋宇,郭鑫鑫,等.用精神分裂症患者皮肤成纤维细胞构建诱导性多潜能干细胞模型[J].新乡医学院学报,2016,33(7):572-576.[doi:10.7683/xxyxyxb.2016.07.006]
　REN Pei-pei,FAN Jin-yu,GUO Xin-xin,et al.Construction of induced pluripotent stem cells model with human dermal fibroblasts of patients with schizophrenia[J].Journal of Xinxiang Medical University,2016,33(7):572-576.[doi:10.7683/xxyxyxb.2016.07.006]
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用精神分裂症患者皮肤成纤维细胞构建诱导性多潜能干细胞模型
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年7
页码:
572-576
栏目:
基础研究
出版日期:
2016-07-05

文章信息/Info

Title:
Construction of induced pluripotent stem cells model with human dermal fibroblasts of patients with schizophrenia
作者:
任佩佩; 樊晋宇; 郭鑫鑫; 原志庆; 林俊堂; 丰慧根
（新乡医学院生命科学技术学院，河南省干细胞与生物治疗工程研究中心，河南　新乡　453003）
Author(s):
REN Pei-pei; FAN Jin-yu; GUO Xin-xin; YUAN Zhi-qing; LIN Jun-tang; FENG Hui-gen
（College of Life Science and Technology，Xinxiang Medical University;Stem Cell and Biological Treatment Engineering Research Center of Henan，Xinxiang 453003，Henan Pvovince，China）
关键词:
诱导性多潜能干细胞; 精神分裂症; 人皮肤成纤维细胞; 细胞模型
Keywords:
induced pluripotent stem cells; schizophrenia; human dermal fibroblasts; cell model
分类号:
R749.3Q819
DOI:
10.7683/xxyxyxb.2016.07.006
文献标志码:
A
摘要:
目的　将精神分裂症患者来源的人皮肤成纤维细胞(HDF)重编程为诱导性多潜能干细胞(iPSCs)，为精神分裂症患者建立iPSCs模型。方法　采用聚合酶链反应技术从pEP4EO2SEM2K质粒上扩增2条目的片段：OCT4-IRES2-SOX2和C-MYC-IRES2-KLF4，将其连接到慢病毒载体pLVX-IRES-mCherry上。构建成功的质粒通过磷酸钙转染法转染293T细胞包装病毒，检测病毒活力后侵染患者皮肤成纤维细胞，将其去分化为诱导性多潜能干细胞，并检测其多能性。结果　测序表明重组慢病毒载体构建成功，并制备了具有良好侵染活力的重组慢病毒。将含4种重编程因子的慢病毒颗粒侵染1例精神分裂症患者的HDF后，HDF的形态逐步发生变化，最终形成典型的iPSCs克隆。结论　成功将1例精神分裂症患者成纤维细胞重编程为iPSCs，为以后建立更多精神分裂症疾病模型奠定基础。
Abstract:
Objective　To reprogrammed the human dermal fibroblasts(HDF) which derived from schizophrenia patient into the induced pluripotent stem cells(iPSCs)，and establish a iPSCs model for patient with schizophrenia.Methods　Two fragments of OCT4-IRES2-SOX2 and C-MYC-IRES2-KLF4 were amplified from the plasmid of pEP4EO2SEM2K by polymerase chain reaction method and the two fragments were connected to the lentiviral vector pLVX-IRES-mCherry.The successfully constructed plasmid were transfected 293T cells by calcium phosphate transfection to package virus.The vitality of virus was detected and then infected the HDF of schizophrenia.Finally，HDF was reprogrammed into the iPSCs and their pluripotentiality was examined.Results　The sequencing results showed that the recombinant lentiviral vectors were constructed successfully and the recombinant virus with good infection activity.HDF cells from a schizophrenia patient were reprogrammed into iPSCs successfully by 4 kinds of reprogramming factors，and the morphology of HDF cells changed gradually，and ultimately formed a typical iPSCs clone.Conclusion　The fibroblasts of one schizophrenia patient was reprogramed into iPSCs successfully，which can lay the foundation for the establishment of more schizophrenia model in the future.

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更新日期/Last Update: 2016-07-05