[1]杨　璐,何广杰,李　静,等.以基质金属蛋白酶-2为靶向的荧光共振能量转移探针制备及其对肝癌细胞的检测[J].新乡医学院学报,2016,33(7):556-560.[doi:10.7683/xxyxyxb.2016.07.002]
　YANG Lu,HE Guang-jie,LI Jing,et al.Preparation of the fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 and its detection of hepatoma cells[J].Journal of Xinxiang Medical University,2016,33(7):556-560.[doi:10.7683/xxyxyxb.2016.07.002]
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以基质金属蛋白酶-2为靶向的荧光共振能量转移探针制备及其对肝癌细胞的检测
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年7
页码:
556-560
栏目:
基础研究
出版日期:
2016-07-05

文章信息/Info

Title:
Preparation of the fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 and its detection of hepatoma cells
作者:
杨　璐1; 何广杰2; 李　静2; 冯思佳1; 赵二趁1; 千新来1; 原志庆1
（1.新乡医学院基础医学院病理学教研室，河南　新乡　453003；2.新乡医学院法医学系，河南　新乡　453003）
Author(s):
YANG Lu1; HE Guang-jie2; LI Jing2; FENG Si-jia1; ZHAO Er-chen1; QIAN Xin-lai1; YUAN Zhi-qing1
(1.Department of Pathology，School of Basic Medical College，Xinxiang Medical University，Xinxiang 453003，Henan Province，China；2.Department of Forensic Medicine，Xinxiang Medical University，Xinxiang 453003，Henan Province，China)
关键词:
肝癌; 基质金属蛋白酶-2; 荧光共振能量转移; 荧光探针; 细胞成像
Keywords:
liver cancer; matrix metalloproteinase-2; fluorescence resonance energy transfer; fluorescent probe; cell imaging
分类号:
R735.7
DOI:
10.7683/xxyxyxb.2016.07.002
文献标志码:
A
摘要:
目的　构建以基质金属蛋白酶-2（MMP-2）为靶向的聚乙二醇（PEG）修饰的荧光共振能量转移探针，探讨其用于肝癌细胞成像检测的可行性。方法　将荧光基团7-(N，N-二乙基氨基)香豆素-3-羧酸琥珀酰亚胺酯通过能够被MMP-2切割的多肽链(GPLGVRGKGG)连接淬灭基团4-［4-(二甲基氨基)苯偶氮］苯甲酸N-丁二酰亚胺酯，构建荧光共振能量转移（FRET)探针。以人肝癌7402细胞和人正常肝细胞L-O2为研究对象，通过细胞成像实验检测人肝癌7402细胞和L-O2细胞中MMP-2的表达水平。结果　体外实验（MMP-2、MMP-1及其他物种对探针的荧光响应）显示了探针对MMP-2的靶向性；体内实验（细胞成像）结果显示，人肝癌7402细胞中MMP-2表达水平高于人正常肝细胞L-O2。结论　该实验制备的探针可以检测人肝癌7402细胞和人正常肝细胞L-O2中MMP-2的表达，将有望用于肝癌的筛查、诊断和预后判断。
Abstract:
Objective　To prepare the polyethylene glycol(PEG) modified fluorescence resonance energy transfer probe targeting matrix metalloproteinase-2 (MMP-2) and to explore the feasibility of it in hepatoma carcinoma cell imaging.Methods　The fluorescence resonance energy transfer probe targeting MMP-2 was prepared by coupling a fluorophore ［7-（diethylamino）-2-oxo-2H-chromene-3-succinimidyl ester］ with a quencher{4-［4-（dimethylamino） phenylazo］benzoic acid N-succinimidyl ester} via a polypeptide chain (GPLGVRGKGG) that could be cleaved by MMP-2.Human hepatoma 7402 cells and normal human liver L-O2 cells were taken as research object and cell imaging was done to detect the MMP-2 expression levels in hepatoma cells.Results　In vitro experiments (the fluorescent probe responses for MMP-2，MMP-1 and other species) showed that the probe targeted MMP-2 and in vivo experiments (cell imaging) results showed that the MMP-2 expression level in human hepatoma 7402 cells was higher than that of normal human liver L-O2 cells.Conclusion　The MMP-2 probe could be used to examine the MMP-2 expression in human hepatoma 7402 cells and human normal liver L-O2 cells，which was expected to be used for hepatoma screening，diagnosis and prognosis.

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更新日期/Last Update: 2016-07-05