[1]宋先璐,黄作平,张　弓,等.X线放射后BALB/c小鼠mCD99L2-A20细胞成瘤率及外周血T淋巴细胞亚群变化[J].新乡医学院学报,2016,33(4):254-256.[doi:10.7683/xxyxyxb.2016.04.002]
　SONG Xian-lu,HUANG Zuo-ping,ZHANG Gong,et al.Rate of tumor formation of mCD99L2-A20 cells and changes of peripheral blood T lymphocyte subsets of BALB/c mice after X-ray radiation[J].Journal of Xinxiang Medical University,2016,33(4):254-256.[doi:10.7683/xxyxyxb.2016.04.002]
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X线放射后BALB/c小鼠mCD99L2-A20细胞成瘤率及外周血T淋巴细胞亚群变化
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年4
页码:
254-256
栏目:
基础研究
出版日期:
2016-04-06

文章信息/Info

Title:
Rate of tumor formation of mCD99L2-A20 cells and changes of peripheral blood T lymphocyte subsets of BALB/c mice after X-ray radiation
作者:
宋先璐1; 黄作平2; 张　弓3; 周同冲1; 韩西群3
(1.广州医科大学附属肿瘤医院放疗科，广东　广州　510095；2.广州武警部队医院肿瘤科，广东　广州　510507；3.南方医科大学病理学系，广东　广州　510515)
Author(s):
SONG Xian-lu1; HUANG Zuo-ping2; ZHANG Gong3; ZHOU Tong-chong1; HAN Xi-qun3
(1.Department of Radiotherapy,Cancer Center of Guangzhou Medical University,Guangzhou 510095,Guangdong Province,China;2.Department of Oncology,Hospital of Guangzhou Armed Police Force,Guangzhou 510507,Guangdong Province,China;3.Department of Pathology,Southern Medical University,Guangzhou 510515,Guangdong Province,China)
关键词:
BALB/c小鼠; X线放射; T淋巴细胞亚群; 成瘤率
Keywords:
BALB/c mice; X-ray radiation; T lymphocyte subsets; tumor formation rate
分类号:
R392.12
DOI:
10.7683/xxyxyxb.2016.04.002
文献标志码:
A
摘要:
目的　探讨X线放射对BALB/c小鼠外周血T淋巴细胞亚群及mCD99L2-A20细胞成瘤率的影响。方法　40只BALB/c小鼠随机分为放射组和对照组，每组20只。放射组小鼠接受X线放射处理，对照组小鼠不接受X线放射处理。2组小鼠每只皮下注射接种mCD99L2-A20细胞悬液100 μL（1×107个细胞），观察2组小鼠的成瘤情况；分别选择对照组、放射组接种mCD99L2-A20后成瘤及未成瘤小鼠各5只，于接种后20 d抽取小鼠外周静脉血，应用流式细胞仪检测T淋巴细胞亚群水平。结果　放射组小鼠成瘤13只（放射后成瘤组），未成瘤7只（放射后未成瘤组），成瘤率为65.0%；对照组小鼠成瘤1只，未成瘤19只，成瘤率为5.0%；放射组小鼠成瘤率显著高于对照组（χ2=15.824，P=0.000）。对照组、放射后未成瘤组和放射后成瘤组小鼠外周血CD3+、CD4+水平比较差异均无统计学意义（F=2.857、2.755，P=0.116、0.104），但3组小鼠外周血CD8+水平比较差异有统计学意义（F=65.087，P=0.000）。放射后未成瘤组和放射后成瘤组小鼠外周血CD8+水平显著低于对照组（t=6.166、12.982，P=0.000），放射后成瘤组小鼠外周血CD8+水平显著低于放射后未成瘤组（t=4.597，P=0.002）。结论　X线放射后BALB/c小鼠接种mCD99L2-A20细胞成瘤率提高，其机制可能与机体免疫功能抑制及细胞毒性T细胞减少有关。
Abstract:
Objective　To investigate the effect of X-ray radiation on the rate of tumor formation of mCD99L2-A20 cells and changes of peripheral blood T lymphocyte subsets in BALB/c mice.Methods　Forty BALB/c mice were randomly divided into radiation group and control group,with twenty rats in each group.The mice in radiation group received X-ray radiation,but the mice in control group did not accept X-ray radiation.mCD99L2-A20 cell suspension(1×107 cells) were inoculated subcutaneously to the BALB/c mice in the two group,then the rate of tumor formation of mice was observed in the two groups.Five normal mice(control group),five mice with tumor formation and five mice without tumor formation(radiation group) were selected.The peripheral blood T lymphocyte subsets were detected by flow cytometer twenty days after inoculation.Results　There were thirteen mice with tumor formation(tumor formation group) and seven mice without tumor formation(no tumor formation group) in radiation group,the tumor formation rate was 65.0%.There were one mice with tumor formation and nineteen mice without tumor formation in control group,the tumor formation rate was 5.0%.The tumor formation rate of mice in radiation group was significantly higher than that in control group(χ2=15.824,P=0.000).There was no significant difference in the levels of CD3+,CD4+ in peripheral blood among control group,no tumor formation group and tumor formation group(F=2.857,2.755;P=0.116,0.104).There was significant difference in peripheral blood CD8+level of mice among the three groups(F=65.087,P=0.000).The CD8+level in peripheral blood of mice in tumor formation group and no tumor formation group was significantly lower than that in control group(t=6.166,12.982;P=0.000).The CD8+level in peripheral blood of mice in tumor formation group was significantly lower than that in no tumor formation group(t=4.597,P=0.002).Conclusion　The tumor formation rate of mCD99L2-A20 cells increase in BALB/c mice after X-ray radiation.The mechanism may be related to the inhibition of immune function and the decrease of cytotoxic T cells.Following radiation immunosuppression of T cells might have been related to suppressed immune function and reduced cytotoxic T cells in the organism.

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更新日期/Last Update: 2016-04-05