[1]刘会芳,李　健,韩双印,等.microRNA-145对人结肠癌Caco-2细胞生物学行为的影响[J].新乡医学院学报,2016,33(3):186-189.[doi:10.7683/xxyxyxb.2016.03.007]
　LIU Hui-fang,LI Jian,HAN Shuang-yin,et al.Effects of microRNA-145 on biological behaviour of colon cancer Caco-2 cells[J].Journal of Xinxiang Medical University,2016,33(3):186-189.[doi:10.7683/xxyxyxb.2016.03.007]
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microRNA-145对人结肠癌Caco-2细胞生物学行为的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年3
页码:
186-189
栏目:
基础研究
出版日期:
2016-03-05

文章信息/Info

Title:
Effects of microRNA-145 on biological behaviour of colon cancer Caco-2 cells
作者:
刘会芳1; 2; 李　健2; 韩双印2; 鲍永华1; 杨万才1
(1.新乡医学院肿瘤与信号传导实验室，河南　新乡　453003；2.河南省人民医院消化内科，河南　郑州　450000)
Author(s):
LIU Hui-fang1; 2; LI Jian2; HAN Shuang-yin2; BAO Yong-hua1; YANG Wan-cai1
(1.Basic Medical College,Xinxiang Medical University,Xinxiang 453003,Henan Province,China;2.Department of Digestive System Diseases,Henan Provincial People′s Hospital,Zhengzhou 450000,Henan Province,China)
关键词:
结肠癌; 微小核糖核酸; 细胞迁移; 细胞增殖; 细胞周期
Keywords:
colon cancer; microRNA; cell proliferation; cell motility; cell cycle
分类号:
R574.62
DOI:
10.7683/xxyxyxb.2016.03.007
文献标志码:
A
摘要:
目的　探讨微小核糖核酸-145(miRNA-145)对人结肠癌细胞系Caco-2细胞迁移、增殖及细胞周期等生物学行为的影响。方法　选用Caco-2细胞并进行培养，用脂质体转染法分别将miRNA-145及阴性对照miRNA过表达的质粒转入Caco-2细胞，分为对照组和miRNA-145组进行实验。在倒置荧光显微镜下观察细胞转染效率；采用划痕试验检测miRNA-145对Caco-2细胞迁移的影响；噻唑蓝法检测miRNA-145对Caco-2细胞增殖的影响；流式细胞术检测miRNA-145对Caco-2细胞周期的影响。结果　与对照组相比，Caco-2细胞过表达miRNA-145后，细胞迁移速度减慢，生长活力明显受到抑制（P<0.05），G1期细胞百分比增高（P<0.05），而G2期及S期细胞百分比则无明显改变（P>0.05）。结论　miRNA-145可抑制Caco-2细胞的迁移和增殖，同时可使Caco-2细胞发生G1期阻滞，减慢细胞周期进程。
Abstract:
Objective　To investigate the effect of microRNA-145(miRNA-145) on proliferation，motility and cell cycle distribution of human colon cancer cell line Caco-2 cells.Methods　Caco-2 cells were cultured and then the miRNA-145 and negative control miRNA mimics were transfected into Caco-2 cells by the liposome transfection method.The experiments were consists of two groups：miRNA-145 group and negative control group.Under the fluorescence microscope observe the cell transfection efficiency.Wound healing assay was used to detect the impact of miRNA-145 on Caco-2 cell motility.The change of proliferation of Caco-2 cells was detected by thiazolyl blue method.The flow cytometry was used to examine the changes of miRNA-145 on Caco-2 cell cycle distribution.Results　Compared to the negative control group，cellular proliferation and motility were significantly inhibited (P<0.05) after over expressing miRNA-145 in Caco-2 cells,and the proportion of G1-stage cells was increased (P<0.05) while the proportion of G2-stage and S-stage cells had no obvious change(P>0.05).Conclusion　miRNA-145 can significantly inhibite Caco-2 cell proliferation and motility，led to the cell cycle arrest at G1-stage and slow down cell cycle progression.

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更新日期/Last Update: 2016-03-05