[1]刘小良,廖鑫鑫,黄　鹏,等.植物提取物碳酸酐酶对肾结石大鼠的影响[J].新乡医学院学报,2016,33(1):012-15.[doi:10.7683/xxyxyxb.2016.01.004]
　LIU Xiao-liang,LIAO Xin-xin,HUANG Peng,et al.Effects of carbonic anhydrase extracts from plant on renal calculi rats[J].Journal of Xinxiang Medical University,2016,33(1):012-15.[doi:10.7683/xxyxyxb.2016.01.004]
点击复制

植物提取物碳酸酐酶对肾结石大鼠的影响
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年1
页码:
012-15
栏目:
基础研究
出版日期:
2016-01-05

文章信息/Info

Title:
Effects of carbonic anhydrase extracts from plant on renal calculi rats
作者:
刘小良; 廖鑫鑫; 黄　鹏; 周海滨; 宋秋元; 曾　泉; 魏胜红; 付　强; 陆文宝; 黄玉清; 张　良; 唐　青
（1.九江学院附属医院泌尿外科，江西　九江　332000；2.九江学院附属医院科研中心，江西　九江　332000)
Author(s):
LIU Xiao-liang1; 2; LIAO Xin-xin1; 2; HUANG Peng1; 2; ZHOU Hai-bin1; 2; SONG Qiu-yuan1; 2; ZENG Quan1; 2; WEI Sheng-hong1; 2; FU Qiang1; 2; LU Wen-bao1; 2; HUANG Yu-qing1; 2; ZHANG Liang1; 2; TANG Qing1; 2
（1.Department of Urology，the Affiliated Hospital of Jiujiang University，Jiujiang 332000，Jiangxi Province，China；2.Scientific Research Center，the Affiliated Hospital of Jiujiang University，Jiujiang 332000，Jiangxi Province，China）
关键词:
碳酸酐酶; 肾结石; 动物模型; 尿排泄
Keywords:
carbonic anhydrase; renal calculi; animal model; urinary excretion
分类号:
R692.4
DOI:
10.7683/xxyxyxb.2016.01.004
文献标志码:
A
摘要:
目的　观察碳酸酐酶(CA)对肾结石大鼠的影响。方法　从植物叶片中提取CA并检测活性。将40只大鼠随机分为 Ⅰ、Ⅱ、Ⅲ 和 Ⅳ 组，每组25只。Ⅰ 组大鼠正常喂养颗粒饲料；Ⅱ 组大鼠每天给予质量分数1％乙二醇+质量分数2％氯化铵2 mL灌胃；Ⅲ组大鼠每天给予质量分数1％乙二醇+质量分数2％氯化铵2 mL+CA 1 mL灌胃；Ⅳ组大鼠每天给予CA 1 mL灌胃。饲养1周后收集4组大鼠24 h尿量，并检测尿液的尿素氮、肌酐、尿酸及钙离子。30 d后处死大鼠，观察肾脏组织结晶形成情况。结果　4组大鼠尿液中的尿素氮、肌酐及尿酸水平比较差异均无统计学意义(P>0.05)；Ⅲ、Ⅳ 组大鼠24 h尿量与 Ⅰ、Ⅱ 组比较差异有统计学意义（P<0.05）；4组大鼠尿钙两两比较差异均有统计学意义（P<0.05）。Ⅱ组可见明显的肾结石结晶；Ⅲ、Ⅳ 组未见明显的肾结石结晶，但可见肾小管结构异常改变。结论　植物提取物CA能够抑制大鼠肾结晶的形成，对肾结石的预防和治疗可能有一定的积极作用。
Abstract:
Objective　To explore the effect of carbonic anhydrase(CA) on the animal model of renal calculi rats.Methods　The CA was extracted from plant and its activity was detected.Forty rats were divided into four groups，with twenty-five rats in each group.The rats in group Ⅰ were fed pelleted diet；the rats in group Ⅱ were fed quality concentration 1% ethylene glycol +quality concentration 2% ammonium chloride 2 mL；the rats in group Ⅲ were fed quality concentration 1% ethylene glycol +quality concentration 2% ammonium chloride 2 mL+ CA 1 mL；the rats in group Ⅳ were fed CA 1 mL.All rats were oral administration per day for 30 days.A week after feeding，24 h urine volume was collected，and the urea nitrogen，creatinine，uric acid and calcium was detected.One month later，rats were sacrificed and the kidney tissues crystal were observed.Results　There was no statistic difference of urine urea nitrogen，creatinine and uric acid levels of rats in the four groups (P>0.05)；there was statistic difference of 24 h urine volume between group Ⅲ，group Ⅳ and group Ⅰ，group Ⅱ (P<0.05)；there was statistic difference of calcium oxalate in urine among the four groups (P<0.05).After rats were sacrificed，renal crystal was observed only in group Ⅱ；no obvious renal crystal was observed in group Ⅲ and group Ⅳ，but abnormal structure of renal tubule was observed.Conclusion　Plant extraction CA can inhibit the formation of renal crystal，may have certain positive role in prevention and treatment of renal calculi.

参考文献/References:

［1］　ROMERO V，AKPINAR H，ASSIMOS D G.Kidney stones：a global picture of prevalence，incidence，and associated risk factors［J］.Rev Urol，2010，12(3)：e86-e96.
［2］　LEAF D E，BUKBERG P R，GOLDFARB D S.Laxative abuse，eating disorders，and kidney stones：a case report and review of the literature［J］.Am J Kidney Dis，2012，60(2)：295-298.
［3］　KHAN S R.Is oxidative stress，a link between nephrolithiasis and obesity，hypertension，diabetes，chronic kidney disease，metabolic syndrome［J］.Urol Res，2012，40(2)：95-112.
［4］　SUPURAN C T.Diuretics：from classical carbonic anhydrase inhibitors to novel applications of the sulfonamides［J］.Curr Pharm Des，2008，14(7)：641-648.
［5］　HURST T K，WANG D，THOMPSON R B，et al.Carbonic anhydrase Ⅱ-based metal ion sensing：advances and new perspectives［J］.Biochim Biophys Acta，2010，1804(2)：393-403.
［6］　赵宗江，于波，宋秀英，等.筛选乙二醇法复制大鼠肾结石模型最佳剂量的比较研究［J］.中国中西医结合肾病杂志，2007，8(11)：628-630.
［7］　MELDRUM N U，ROUGHTON F J.Carbonic anhydrase.Its preparation and properties［J］.J Physiol，1933，80(2)：113-142.
［8］　SUPURAN C T.Carbonic anhydrase inhibitors［J］.Bioorg Med Chem Lett，2010，20(12)：3467-3474.
［9］　PEREIRA P C，MIRANDA D M，OLIVEIRA E A，et al.Molecular pathophysiology of renal tubular acidosis［J］.Curr Genomics，2009，10(1)：51-59.
［10］　SKOLARIKOS A，STRAUB M，KNOLL T，et al.Metabolic evaluation and recurrence prevention for urinary stone patients：EAU guidelines［J］.Eur Urol，2015，67(4)：750-763.
［11］　HAYMANN J P.Metabolic disorders：stones as first clinical manifestation of significant diseases［J］.World J Urol，2015，33(2)：187-192.
［12］　WU T J，YAN M T，CHAU T，et al.Hypokalemic paralysis in a girl with dental and renal calculi.Distal renal tubular acidosis (RTA)［J］.Pediatr Nephrol，2013，28(5)：731，733-736.
［13］　HAYMANN J P.Metabolic disorders：stones as first clinical manifestation of significant diseases［J］.World J Urol，2015，33(2)：187-192.
［14］　DELLORTO V G，BELOTTI E A，GOEGGEL-SIMONETTI B，et al.Metabolic disturbances and renal stone promotion on treatment with topiramate：a systematic review［J］.Br J Clin Pharmacol，2014，77(6)：958-964.
［15］　MIRZA N S，ALFIREVIC A，JORGENSEN A，et al.Metabolic acidosis with topiramate and zonisamide：an assessment of its severity and predictors［J］.Pharmacogenet Genomics，2011，21(5)：297-302.
［16］　HANCHANALE V S，BIYANI C S，BROWNING A J，et al.Topiramate and the risk of urolithiasis［J］.BJU Int，2011，107(3)：352-354.
［17］　VEGA D，MAALOUF N M，SAKHAEE K.Increased propensity for calcium phosphate kidney stones with topiramate use［J］.Expert Opin Drug Saf，2007，6(5)：547-557.
［18］　NAKAI H，BYERS M G，VENTA P J，et al.The gene for human carbonic anhydrase Ⅱ(CA2) is located at chromosome 8q22［J］.Cytogenet Cell Genet，1987，44(4)：234-235.
［19］　SODA H.Carbonic anhydrase Ⅱ deficiency syndrome：clinico-pathological，biochemical and molecular studies［J］.Kurume Med J，1994，41(4)：233-240.
［20］　SLY W S，HEWETT-EMMETT D，WHYTE M P，et al.Carbonic anhydrase Ⅱ deficiency identified as the primary defect in the autosomal recessive syndrome of osteopetrosis with renal tubular acidosis and cerebral calcification［J］.Proc Natl Acad Sci USA，1983，80(9)：2752-2756.
［21］　李为，曾宪东，栗茂腾，等.微藻及其碳酸酐酶对石灰岩土壤系统中钙元素迁移的驱动作用实验研究［J］.矿物岩石地球化学通报，2011，30(3)：261-264.
［22］　LI W，YU L J，WU Y，et al.Enhancement of Ca2+ release from limestone by microbial extracellular carbonic anhydrase［J］.Bioresour Technol，2007，98(4)：950-953.

相似文献/References:

[1]刘志斌,张正伟,杨佳全,等.复杂性肾结石开放手术取石的术式选择[J].新乡医学院学报,2011,28(06):000.
[2]庞松强,罗功唐,宋　瑶. 经皮肾镜钬激光碎石术治疗肾铸型结石疗效观察[J].新乡医学院学报,2015,32(08):768.
[3]周真文,毕满华,蒋　方,等.经皮肾镜碎石术与经尿道输尿管软镜碎石术治疗肾结石疗效比较[J].新乡医学院学报,2021,38(3):289.[doi:10.7683/xxyxyxb.2021.03.018]
　ZHOU Zhenwen,BI Manhua,JIANG Fang,et al.Comparison of the effect between percutaneous nephrolithotomy and transurethral flexible ureteroscopic lithotripsy in the treatment of renal calculus[J].Journal of Xinxiang Medical University,2021,38(1):289.[doi:10.7683/xxyxyxb.2021.03.018]
[4]王　磊,马　玲,乔庆东,等.输尿管软镜碎石术和体外冲击波碎石术治疗小于20 mm肾结石的临床疗效及影响因素分析[J].新乡医学院学报,2019,36(12):1141.[doi:10.7683/xxyxyxb.2019.12.009]
　WANG Lei,MA Ling,QIAO Qing-dong,et al.Clinical effect and influencing factors of the flexible ureteroscopic lithotripsy and extracorporeal shock wave lithotripsy for diameter 20 mm renal calculi[J].Journal of Xinxiang Medical University,2019,36(1):1141.[doi:10.7683/xxyxyxb.2019.12.009]
[5]张　刚,李　航,韩　湘,等.局部麻醉与连续硬膜外麻醉在微创经皮肾镜取石术中的应用效果比较[J].新乡医学院学报,2018,35(5):428.[doi:10.7683/xxyxyxb.2018.05.019]
　ZHANG Gang,LI Hang,HAN Xiang,et al.Comparison of the effect of local anesthesia and continuous epidural anesthesia in minimally invasive percutaneous nephrolithotomy[J].Journal of Xinxiang Medical University,2018,35(1):428.[doi:10.7683/xxyxyxb.2018.05.019]
[6]朱　峰,范毛川,陈帅奇,等.输尿管软镜与微创经皮肾镜碎石术治疗直径≤2 cm肾结石的对比分析[J].新乡医学院学报,2016,33(6):533.[doi:10.7683/xxyxyxb.2016.06.025]
　ZHU Feng,FAN Mao-chuan,CHEN Shuai-qi,et al.Comparative analysis of the flexible ureteroscopic lithotripsy and minimally inasive percutaneous nephrolithotomy for renal calculi ≤ 2 cm in size[J].Journal of Xinxiang Medical University,2016,33(1):533.[doi:10.7683/xxyxyxb.2016.06.025]

更新日期/Last Update: 2016-01-05