[1]张博汝,魏柏,石晓雅,等.生物钟基因Bmal1对结肠癌细胞增殖的影响[J].新乡医学院学报,2022,39(11):1001-1005.[doi:10.7683/xxyxyxb.2022.11.001]
　ZHANG Boru,WEI Bai,SHI Xiaoya,et al.Effect of circadian clock gene Bmal1 on the proliferation of colon cancer cell[J].Journal of Xinxiang Medical University,2022,39(11):1001-1005.[doi:10.7683/xxyxyxb.2022.11.001]
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生物钟基因Bmal1对结肠癌细胞增殖的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
39
期数:
2022年11
页码:
1001-1005
栏目:
基础研究
出版日期:
2022-11-05

文章信息/Info

Title:
Effect of circadian clock gene Bmal1 on the proliferation of colon cancer cell
作者:
张博汝; 魏柏; 石晓雅; 谢梦丽
（华中科技大学同济医学院附属梨园医院肿瘤科，湖北　武汉　430077）
Author(s):
ZHANG Boru; WEI Bai; SHI Xiaoya; XIE Mengli
(Department of Oncology,Liyuan Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430077,Hubei Province,China)
关键词:
Bmal1基因; 结肠癌细胞; 缺氧诱导因子-1α; 血管内皮生长因子
Keywords:
Bmal1 gene; colon cancer cell; hypoxia-inducible factor-1α; vascular endothelial growth factor
分类号:
R574.62
DOI:
10.7683/xxyxyxb.2022.11.001
文献标志码:
A
摘要:
目的　探讨生物钟基因Bmal1基于缺氧诱导因子-1α（HIF-1α）/血管内皮生长因子（VEGF）信号通路对结肠癌细胞增殖的影响。方法　将对数生长期结肠癌MC38细胞随机分为对照组和Bmal1基因沉默组，2组细胞按照HiPerFect 转染试剂说明书分别转染阴性对照干扰小RNA（siRNA）和Bmal1 siRNA。细胞转染后0、24、48 h，采用细胞计数试剂盒-8（cell counting kit-8，CCK-8）法检测2组细胞增殖能力；细胞转染后48 h，采用实时荧光定量聚合酶链式反应法检测2组细胞中Bmal1、HIF-1α、VEGF mRNA的相对表达量，Western blot法检测2组细胞Bmal1、HIF-1α、VEGF蛋白的相对表达量。结果　转染后0 h， 2组细胞增殖能力比较差异无统计学意义（P>0.05）；转染后24、48 h，Bmal1基因沉默组细胞增殖能力显著低于对照组（P<0.05）。细胞转染后48 h，Bmal1基因沉默组细胞中Bmal1 mRNA和蛋白相对表达量显著低于对照组（P<0.05），Bmal1基因沉默组细胞中HIF-1α和VEGF的mRNA和蛋白相对表达量显著低于对照组(P<0.05)。 结论　沉默Bmal1基因可抑制结肠癌细胞的增殖，其机制可能与抑制HIF-1α/VEGF信号通路有关。
Abstract:
Objective　To investigate the effect of circadian clock gene Bmal1 on the proliferation of colon cancer cell based on hypoxia-inducible factor-1α (HIF-1α)/vascular endothelial growth factor (VEGF) signaling pathway.Methods　MC38 cells of colon cancer in logarithmic growth stage were randomly divided into control group and Bmal1 gene silencing group.The cells in the two groups were transfected with negative control small interfering RNA (siRNA) and Bmal1 siRNA according to the instructions of HiPerFect transfection reagent,respectively.At 0,24,48 hours after transfection,the proliferation ability of cells in the two groups was detected by the cell counting kit-8 (CCK-8) assay.At 48 hours after transfection,the relative expressions of Bmal1,HIF-1α and VEGF mRNA in cells in the two groups were detected by real-time fluorescence quantitative polymerase chain reaction,the relative expressions of Bmal1,HIF-1α and VEGF protein in the cells in the two groups were detected by Western Blot.Results　There was no significant difference in the cell proliferation ability between the two groups at 0 hour after transfection (P>0.05).At 24 and 48 hours after transfection,the proliferation activity of cells in the Bmal1 gene silencing group was significantly lower than that in the control group (P<0.05).At 48 hours after transfection,the relative expressions of Bmal1 mRNA and protein in cells in the Bmal1 gene silencing group were significantly lower than those in the control group (P<0.05),and the relative expressions of HIF-1α and VEGF mRNA and protein in cells in the Bmal1 gene silencing group were significantly lower than those in the control group (P<0.05).Conclusion　Bmal1 gene silencing can inhibit the proliferation of colon cancer cells,and the mechanism may be related to the inhibition of HIF-1α/VEGF signaling pathway.

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更新日期/Last Update: 2022-11-05