[1]高　健.支气管哮喘患儿血清小窝蛋白-1水平和叉头框蛋白M1基因表达与气道重塑的相关性[J].新乡医学院学报,2022,39(2):155-159.[doi:10.7683/xxyxyxb.2022.02.011]
　GAO Jian.Correlation between serum caveolin-1,forkhead box M1 gene expression and airway remodeling in children with bronchial asthma[J].Journal of Xinxiang Medical University,2022,39(2):155-159.[doi:10.7683/xxyxyxb.2022.02.011]
点击复制

支气管哮喘患儿血清小窝蛋白-1水平和叉头框蛋白M1基因表达与气道重塑的相关性
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
39
期数:
2022年2
页码:
155-159
栏目:
临床研究
出版日期:
2022-02-05

文章信息/Info

Title:
Correlation between serum caveolin-1,forkhead box M1 gene expression and airway remodeling in children with bronchial asthma
作者:
高　健
（河南省儿童医院/郑州儿童医院/郑州大学附属儿童医院/郑州市儿童感染与免疫重点实验室，河南　郑州　450018）
Author(s):
GAO Jian
(Henan Children′s Hospital/Zhengzhou Children′s Hospital/Children′s Hospital Affiliated to Zhengzhou University/Zhengzhou Key Laboratory of Children′s Infection and Immunity,Zhengzhou 450018,Henan Province,China)
关键词:
支气管哮喘; 小窝蛋白-1; 叉头框蛋白M1; 气道重塑; 肺功能
Keywords:
bronchial asthma; caveolin-1; forkhead box M1; airway remodeling; lung function
分类号:
R725.6
DOI:
10.7683/xxyxyxb.2022.02.011
文献标志码:
A
摘要:
目的　探讨支气管哮喘患儿血清小窝蛋白-1（Cav-1）水平、叉头框蛋白M1（FOXM1）基因表达与气道重塑的相关性。方法　选择2020年1月至2021年7月郑州大学附属儿童医院收治的178例支气管哮喘患儿为研究对象，其中急性发作期92例（急性发作组）、临床缓解期86例（临床缓解组）；急性发作期患儿根据病情程度分为轻度组（n=47）和中重度组（n=45）。采用酶联免疫吸附法检测患儿血清Cav-1水平，实时荧光定量聚合酶链反应法检测血清中FOXM1 mRNA表达；应用肺功能检测仪检测患儿第1秒用力呼气量（FEV1）、用力肺活量（FVC），并计算FEV1/FVC；应用胸部CT检查患儿气道重塑情况，计算支气管管壁厚度与外直径的比值（T/D）、支气管壁占支气管总横截面积的百分比（WA）。结果　急性发作组患儿血清Cav-1水平显著低于临床缓解组，FOXM1 mRNA相对表达量显著高于临床缓解组（P<0.05）。急性发作组患儿FEV1、FEV1/FVC显著低于临床缓解组，T/D、WA显著高于临床缓解组（P<0.05）。中重度组患儿血清Cav-1水平及FEV1、FEV1/FVC显著低于轻度组，血清中FOXM1 mRNA相对表达量及T/D、WA显著高于轻度组（P<0.05）。Pearson分析结果显示，急性发作期支气管哮喘患儿血清Cav-1水平与FOXM1 mRNA表达呈负相关（r=-0.512，P<0.05）；血清Cav-1水平与FEV1、FEV1/FVC呈正相关（r=0.516、0.499，P<0.05），与T/D、WA呈负相关（r=-0.508、-0.514，P<0.05）；血清中FOXM1 mRNA表达水平与FEV1、FEV1/FVC呈负相关（r=-0.494、-0.487，P<0.05），与T/D、WA呈正相关（r=0.504、0.512，P<0.05）。结论　支气管哮喘患儿血清中Cav-1水平降低，FOXM1基因表达上调，二者可能参与了气道重塑的发生与发展；血清Cav-1和FOXM1基因表达水平对评估支气管哮喘患儿病情严重程度有重要价值。
Abstract:
Objective　To investigate the correlation between serum caveolin-1 (Cav-1),forkhead box M1 (FOXM1) gene expression and airway remodeling in children with bronchial asthma.Methods　A total of 178 children with bronchial asthma treated in the Children′s Hospital Affiliated to Zhengzhou University from January 2020 to July 2021 were selected as the research subjects,including 92 cases in acute attack stage (acute attack group) and 86 cases in clinical remission stage (clinical remission group).The children in acute attack stage were divided into mild group (n=47) and moderate-severe group (n=45).The level of serum Cav-1 was detected by enzyme-linked immunosorbent assay,and the expression of FOXM1 mRNA was detected by real-time fluorescence quantitative polymerase chain reaction.The forced expiratory volume in one second (FEV1) and forced vital capacity (FVC) were measured by pulmonary function tester,and the FEV1/FVC was calculated.The airway remodeling of children was examined by chest CT,and the ratio of bronchial wall thickness to outer diameter (T/D) and the percentage of bronchial wall in the total cross-sectional area of bronchus (WA) were calculated.Results　The level of serum Cav-1 in the acute attack group was significantly lower than that in the clinical remission group,and the relative expression of FOXM1 mRNA in the acute attack group was significantly higher than that in the clinical remission group (P<0.05).The FEV1 and FEV1 / FVC in the acute attack group were significantly lower than those in the clinical remission group,and T/D and WA in the acute attack group were significantly higher than those in the clinical remission group (P<0.05).The serum Cav-1 level,FEV1 and FEV1/FVC in the moderate-severe group were significantly lower than those in the mild group,and the relative expression of serum FOXM1 mRNA,T/D and WA in the moderate-severe group were significantly higher than those in the mild group (P<0.05).Pearson analysis showed that serum Cav-1 level was negatively correlated with FOXM1 mRNA expression in children with acute exacerbation of bronchial asthma (r=-0.512,P<0.05);the serum Cav-1 level was positively correlated with FEV1 and FEV1/FVC (r=0.516,0.499;P<0.05),and the serum Cav-1 level was negatively correlated with T/D and WA (r=-0.508，-0.514;P<0.05);the expression level of FOXM1 mRNA in serum was negatively correlated with FEV1 and FEV1/FVC (r=-0.494,-0.487;P<0.05),and it was positively correlated with T/D and WA (r=0.504,0.512;P<0.05).Conclusion　The serum Cav-1 level decreased and the expression of FOXM1 gene increased in children with bronchial asthma,they may be involved in the occurrence and development of airway remodeling.The levels of serum Cav-1 and FOXM1 gene expression are of great value in evaluating the severity of children with bronchial asthma.

参考文献/References:

［1］　MANDLIK D S,MANDLIK S K.New perspectives in bronchial asthma:pathological,immunological alterations,biological targets,and pharmacotherapy［J］.Immunopharmacol Immunotoxicol,2020,42(6):521-544.
［2］　常兴，张恬，颜培正，等.NF-κB与PI3K-Akt-mTOR信号通路在支气管哮喘病理机制中的研究进展［J］.现代中西医结合杂志，2019，28（34）：3863-3866.
CHANG X,ZHANG T,YAN P Z,et al.Research progress of NF-κB and PI3K-Akt-mTOR signaling pathway in the pathological mechanism of bronchial asthma［J］.Mod J Int Trad Chin West Med,2019,28(34):3863-3866.
［3］　BANNO A,REDDY A T,LAKSHMI S P,et al.Bidirectional interaction of airway epithelial remodeling and inflammation in asthma［J］.Clin Sci (Lond),2020,134(9):1063-1079.
［4］　于卫民，姬建胜，王冬冬.RNA干扰Caveolin-1基因表达抑制食管癌细胞增殖侵袭及PI3K/AKT信号通路的研究［J］.中国地方病防治杂志,2018,33(3):357-357.
YU W M,JI J S,WANG D D.Inhibition of caveolin-1 gene expression by RNA interference on proliferation and invasion of esophageal cancer cells and PI3K / Akt signaling pathway［J］.Chin J Ctrl Endem Dis,2018,33(3):357-357.
［5］　LI H T,YE C,ZHOU M,et al.Moxifloxacin suppresses airway inflammation and modulates expression of caveolin-1 and flotillin-1 in airway smooth muscle cells of asthmatic rats［J］.Ann Transl Med,2019,7(18):469-469.
［6］　ZHU X,LU K Y,CAO L Y,et al.FoxM1 is upregulated in osteosarcoma and inhibition of FoxM1 decreases osteosarcoma cell proliferation,migration,and invasion［J］.Cancer Manag Res,2020,12(1):9857-9867.
［7］　WU G H,YANG L T,XU Y,et al.FABP4 induces asthmatic airway epithelial barrier dysfunction via ROS-activated FoxM1［J］.Biochem Biophys Res Commun,2018,495(1):1432-1439.
［8］　中华医学会呼吸病学分会哮喘学组，中国哮喘联盟.支气管哮喘急性发作评估及处理中国专家共识［J］.中华内科杂志，2018，57（1）：4-14.
ASTHMA GROUP OF CHINESE THORACIC,CHINA ASTHMA ALLIANCE.The Chinese experts′ consensus on the evaluation and management of asthma exacerbation［J］.Chin J Intern Med,2018,57(1):4-14.
［9］　MILOTA T,BLOOMFIELD M,PARACKOVA Z,et al.Bronchial asthma and bronchial hyperresponsiveness and their characteristics in patients with common variable immunodeficiency［J］.Int Arch Allergy Immunol,2019,178(2):192-200.
［10］　王宇，陈亚飞，张瑞.支气管哮喘患者血清中转化生长因子β1表达水平与气道重塑的相关性分析［J］.现代诊断与治疗，2021，32（1）：122-124.
WANG Y,CHEN Y F,ZHANG R.Correlation between serum transforming growth factor β1 expression and airway remodeling in patients with bronchial asthma［J］.Mod Diagn Treat,2021,32(1):122-124.
［11］　INOUE H,ITO I,NIIMI A,et al.CT-assessed large airway involvement and lung function decline in eosinophilic asthma:the association between induced sputum eosinophil differential counts and airway remodeling［J］.J Asthma,2016,53(9):914-921.
［12］　蒋凌志，许丹媛，杨志雄.支气管哮喘患者血清25-(OH)D3、TGF-β1水平与气道重塑的关系［J］.山东医药，2018，58（12）：54-56.
JIANG L Z,XU D Y,YANG Z X.Relationship between serum 25-(OH)D3,TGF-β1 levels and airway remodeling in patients with bronchial asthma［J］.Shandong Med J,2018,58(12):54-56.
［13］　程丹，胡克.气道上皮屏障功能异常在支气管哮喘发病中的作用［J］.国际呼吸杂志，2020，40（2）：146-150.
CHENG D,HU K.Role of airway epithelial barrier dysfunction in bronchial asthma［J］.Int J Respir,2020,40(2):146-150.
［14］　FREY A,LUNDING L P,EHLERS J C,et al.More than just a barrier:the immune functions of the airway epithelium in asthma pathogenesis［J］.Front Immunol,2020,11(1):761-783.
［15］　ZHANG M M,CHEN G,WANG Y,et al.Polymorphisms in the airway epithelium related genes CDHR3 and EMSY are associated with asthma susceptibility［J］.BMC Pulm Med,2020,20(1):305-314.
［16］　WANG I,CHIU Y,CHEN W,et al.Increased levels of FOXM1 transcription factor in bronchioalveolar stem cells promote epithelial cell repopulation in injured mouse airway［J］.FASEB J,2018,32(1):817.
［17］　王文慧，李洪艳，屈超，等.小窝蛋白-1:肿瘤细胞氧化应激靶标［J］.生理学报，2019，71（5）：116-122.
WANG W H,LI H Y,QU C,et al.Caveolin-1:oxidative stress target in tumor cells［J］.Acta Physiologica Sinica,2019,71(5):116-122.
［18］　WANG J,SHANG Y X,CAI X X,et al.Vasoactive intestinal peptide inhibits airway smooth muscle cell proliferation in a mouse model of asthma via the ERK1/2 signaling pathway［J］.Exp Cell Res,2018,364(2):168-174.
［19］　李慧婷，赵杰，李海泉，等.莫西沙星可浓度依赖性增加人气道平滑肌细胞Caveolin-1的表达［J］.中外医疗，2019，38（9）：11-13.
LI H T,ZHAO J,LI H Q,et al.Moxifloxacin increasing the expression of caveolin-1 of human airway smooth muscle cell in concentration-dependent manner［J］.Chin Foreign Med Treat,2019,38(9):11-13.

相似文献/References:

[1]杜荣,郭悦鹏,陈永凤,等.误诊为支气管哮喘的气管肿瘤10例[J].新乡医学院学报,1996,13(01):090.
[2]张永辉,刘宗花,杜红丽,等.中药泡桐花浸膏对哮喘豚鼠肺组织作用的病理学研究[J].新乡医学院学报,2002,19(06):473.
[3]张永辉,宋伟涛,刘宗花,等.中药泡桐花浸膏防治支气管哮喘的实验性研究[J].新乡医学院学报,2002,19(03):186.
[4]王玉梅.联用沙丁胺醇和溴化异丙托品雾化吸入治疗支气管哮喘38例[J].新乡医学院学报,2002,19(04):314.
[5]张士奎,张道远.哮喘90 例对糖皮质激素气雾剂依从性调查[J].新乡医学院学报,2003,20(01):045.
[6]张士奎。 张道远.哮喘90例对糖皮质激素气雾剂依从性调查[J].新乡医学院学报,2003,20(01):046.
[7]王世英,忽新刚,吴春芳,等.支气管哮喘患者血清免疫球蛋白E和支气管肺泡灌洗液中白介素8测定及意义[J].新乡医学院学报,2009,26(01):056.
[8]陈　芳.小剂量罗红霉素对支气管哮喘患儿细胞免疫功能及炎症因子水平的影响[J].新乡医学院学报,2020,37(1):068.[doi:10.7683/xxyxyxb.2020.01.016]
　CHEN Fang.Effect of low-dose roxithromycin on cellular immune function and inflammatory factors in children with bronchial asthma[J].Journal of Xinxiang Medical University,2020,37(2):068.[doi:10.7683/xxyxyxb.2020.01.016]
[9]韩亚军,贾　新,王晓明.沙美特罗替卡松粉吸入剂联合氨茶碱注射液治疗老年支气管哮喘疗效观察[J].新乡医学院学报,2020,37(5):433.[doi:10.7683/xxyxyxb.2020.05.008]
　HAN Yajun,JIA Xin,WANG Xiaoming.Effect of salmeterol xinafoate and fluticasone propionate powder for inhalation combined with aminophylline injection in the treatment of elderly patients with bronchial asthma[J].Journal of Xinxiang Medical University,2020,37(2):433.[doi:10.7683/xxyxyxb.2020.05.008]
[10]曹利静,严东星,李林静,等.百令胶囊对支气管哮喘患者呼吸道重塑及肺功能的影响[J].新乡医学院学报,2019,36(5):475.[doi:10.7683/xxyxyxb.2019.05.018]
　CAO Li-jing,YAN Dong-xing,LI Lin-jing,et al.Effect of Bailing capsule on respiratory tract remodeling and pulmonary function in patients with bronchial asthma[J].Journal of Xinxiang Medical University,2019,36(2):475.[doi:10.7683/xxyxyxb.2019.05.018]

更新日期/Last Update: 2022-02-05