[1]王文茜,开　悦,刘　虎,等.高脂饮食对小鼠不同组织中成纤维细胞生长因子21及其受体表达的影响[J].新乡医学院学报,2020,37(4):318-322.[doi:10.7683/xxyxyxb.2020.04.004]
　WANG Wenqian,KAI Yue,LIU Hu,et al.Effect of high fat diet on the expression of fibroblast growth factor 21 and its receptors in different tissues of mice[J].Journal of Xinxiang Medical University,2020,37(4):318-322.[doi:10.7683/xxyxyxb.2020.04.004]
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高脂饮食对小鼠不同组织中成纤维细胞生长因子21及其受体表达的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
37
期数:
2020年4
页码:
318-322
栏目:
基础研究
出版日期:
2020-04-05

文章信息/Info

Title:
Effect of high fat diet on the expression of fibroblast growth factor 21 and its receptors in different tissues of mice
作者:
王文茜1; 2; 开　悦1; 2; 刘　虎1; 2; 王玉冰1; 2; 宋向凤1; 2
（1.新乡医学院免疫学教研室，河南　新乡　453003；2.新乡市肿瘤疫苗及免疫治疗重点实验室，河南　新乡　453003）
Author(s):
WANG Wenqian1; 2; KAI Yue1; 2; LIU Hu1; 2; WANG Yubing1; 2; SONG Xiangfeng1; 2
（1.Department of Immunology，Xinxiang Medical University，Xinxiang 453003，Henan Province，China；2.Xinxiang City Key Laboratory of Tumor Vaccine and Immunotherapy，Xinxiang 453003，Henan Province，China）
关键词:
高脂饮食; 肥胖; 成纤维细胞生长因子21; 成纤维细胞生长因子受体
Keywords:
high fat diet; obesity; fibroblast growth factor 21; fibroblast growth factor receptor
分类号:
R589.2
DOI:
10.7683/xxyxyxb.2020.04.004
文献标志码:
A
摘要:
目的　探讨成纤维细胞生长因子21（FGF21）及其受体在高脂饮食诱导的肥胖小鼠体内不同组织中的表达。方法　选取6~8周龄C57BL/6雄性小鼠10只，适应性饲养1周后随机分为正常饮食组和高脂饮食组，每组5只。正常饮食组小鼠给予普通饲料；高脂饮食组小鼠给予高脂饲料，自由摄食及饮水，连续饲养10周，建立高脂饮食肥胖模型小鼠。采用酶联免疫吸附试验检测2组小鼠血清FGF21水平，实时荧光定量聚合酶链反应检测2组小鼠附睾脂肪组织、肝脏和骨骼肌组织中FGF21、FGF21受体（FGFR）和辅助受体β-Klotho表达，苏木精-伊红染色观察附睾脂肪组织和肝脏组织病理变化。结果　喂养10周后，高脂饮食组小鼠体质量约为正常饮食组小鼠体质量的1.3倍，且体质量增量显著高于正常饮食组（P＜0.01），高脂饮食诱导的肥胖小鼠模型制备成功。小鼠附睾脂肪组织结构正常，形态规则，细胞轮廓清晰，细胞核呈蓝色贴于细胞膜周边；正常饮食组小鼠附睾脂肪组织中脂肪细胞大小正常；而高脂饮食组小鼠附睾脂肪组织中脂肪细胞体积明显增大。正常饮食组小鼠肝脏组织结构正常；高脂饮食组小鼠肝组织内部分区域脂肪空泡显著增多，肝脏细胞表现出脂肪变性且周围有炎症细胞浸润。与正常饮食组比较，高脂饮食组小鼠血清FGF21水平显著升高（P＜0.05），附睾脂肪组织中FGF21 mRNA相对表达量显著降低（P＜0.05），肝脏组织和骨骼肌组织中FGF21 mRNA相对表达量升高（P＜0.05）；高脂饮食组小鼠附睾脂肪组织、肝脏组织和骨骼肌组织中FGFR、β-Klotho mRNA相对表达量显著低于正常饮食组（P＜0.05，P＜0.01）。结论　高脂饮食诱导的肥胖小鼠体内不同组织中FGF21及其受体FGFR和β-Klotho表达失调。
Abstract:
Objective　To investigate the expression of fibroblast growth factor 21(FGF21) and its receptors in different tissues of obese mice induced by high fat diet.Methods　Ten male C57BL/6 mice aged 6-8 weeks were randomly divided into normal diet group and high fat diet group after 1 week of adaptive breeding，with 5 mice in each group.The mice in the normal diet group were fed with common feed;the mice in the high fat diet group were fed with high fat diet;all mice freely fed and drank for 10 weeks to establish the high fat diet obese model.The level of FGF21 in the serum of mice in the two groups was detected by enzyme-linked immunosorbent assay；the expression of FGF21 and its receptors FGFR and assistant receptor of β-Klotho in adipose tissues of epididymis，liver and muscle tissues were detected by real-time fluorescent quantitative polymerase chain reaction;the pathological changes of liver tissues and adipose tissues were observed by hematoxylin and eosin staining.Results　After 10 weeks of feeding，the body weight of mice in the high fat diet group reached about 130% of the body weight of mice in the normal diet group and the body weight increment was significantly higher than that in the normal diet group (P<0.01)，which indicated that the high fat diet-induced obese mouse model was successfully prepared.The structure of the adipose tissue of the epididymis of mice was normal，the shape was regular，the cell outlin was clear，and the nucleus was blue and attached to the periphery of the cell membrane;the size of adipocytes in the epididymal adipose tissue of mice in the normal diet group was normal，while the volume of adipocytes in the epididymal adipose tissue of mice in the high fat diet group increased significantly.In the normal diet group,the liver tissue structure was normal;in the high fat diet group，the number of fat vacuoles in some areas of the liver tissue increased significantly，and the liver cells showed steatosis with inflammatory cell infiltration.Compared with the normal diet group，the serum level of FGF21 in the high fat diet group increased significantly (P<0.05)，the relative expression of FGF21 mRNA in epididymal adipose tissue decreased significantly (P<0.05)，and the relative expression of FGF21 mRNA in liver tissue and skeletal muscle tissue increased significantly (P<0.05).The relative expression levels of FGFR and β-Klotho mRNA in epididymal adipose tissue，liver tissue and skeletal muscle tissue of mice in the high fat diet group were significantly lower than those in the normal diet group (P<0.05，P<0.01).Conclusion　The expression of FGF21 and its receptors FGFR and β-Klothoare are dysregulated in different tissues of obese mice induced by high fat diet.

参考文献/References:

［1］　BONDURANT L D，POTTHOFF M J.Fibroblast growth factor 21：a versatile regulator of metabolic homeostasis［J］.Annu Rev Nutr，2018，38：173-196.
［2］　LI H，WU G，FANG Q，et al.Fibroblast growth factor 21 increases insulin sensitivity through specific expansion of subcutaneous fat［J］.Nat Commun，2018，9(1)：272.
［3］　DEGIROLAMO C，SABBA C，MOSCHETTA A.Therapeutic potential of the endocrine fibroblast growth factors FGF19，FGF21 and FGF23［J］.Nat Rev Drug Discov，2016，15(1)：51-69.
［4］　JELENIK T，DILLE M，MLLER-LHLHOFF S，et al.FGF21 regulates insulin sensitivity following long-term chronic stress［J］.Mol Metab，2018，16：126-138.
［5］　崔丽梅，吕纳强.肥胖的疾病特征［J］.中国比较医学杂志，2019，29(10)：22-25，111.
［6］　NISHIMURA T，NAKATAKE Y，KONISHI M，et al.Identification of a novel FGF，FGF-21，preferentially expressed in the liver［J］.Biochim Biophys Acta，2000，1492(1)：203-206.
［7］　STAIGER H，KEUPER M，BERTI L，et al.Fibroblast growth factor 21-metabolic role in mice and men［J］.Endocr Rev，2017，38(5)：468-488.
［8］　GENG L,LIAO B,JIN L,et al.Exercise alleviates obesity-induced metabolic dysfunction via enhancing FGF21 sensitivity in adipose tissues［J］.Cell Rep,2019,26(10):2738-2752.
［9］　GAO R Y，HSU B G，WU D A，et al.Serum fibroblast growth factor 21 levels are positively associated with metabolic syndrome in patients with type 2 diabetes［J］.Int J Endocrinol，2019，2019：5163245.
［10］　TEZZE C，ROMANELLO V，SANDRI M.FGF21 as modulator of metabolism in health and disease［J］.Front Physiol，2019，10：419.
［11］　CHA J Y,NEPALI S,LEE H Y,et al.Chrysanthemum indicum L.ethanol extract reduces high-fat diet-induced obesity in mice［J］.Exp Ther Med，2018,15(6):5070-5076.
［12］　TANAJAK P，PONGKAN W，CHATTIPAKORN S C，et al.Increased plasma FGF21 level as an early biomarker for insulin resistance and metabolic disturbance in obese insulin-resistant rats［J］.Diab Vasc Dis Res，2018，15(3)：263-269.
［13］　田思聪.莱菔硫烷对肝脏脂肪滴形成的影响及MAM区的调控机制［D］.哈尔滨：哈尔滨工业大学，2019.
［14］　杨国灿，刘琪.FGF-21对酒精性肝病的保护作用及其机制研究［J］.中国免疫学杂志，2019，35(14)：1686-1688，1693.
［15］　BONDURANT L D，AMEKA M，NABER M C，et al.FGF21 regulates metabolism through adipose-dependent and -independent mechanism［J］.Cell Metab，2017，25(4)：935-944.
［16］　MARKAN K R.Defining "FGF21 resistance" during obesity：controversy，criteria and unresolved questions［J］.F1000 Res，2018，7：289.
［17］　HALE C，CHEN M M，STANISLAUS S，et al.Lack of overt FGF21 resistance in two mouse models of obesity and insulin resistance［J］.Endocrinology，2012，153(1)：69-80.

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更新日期/Last Update: 2020-04-05