[1]彭双春,陈米炼,欧册华.CD95在阿托伐他汀抗大鼠三叉神经痛中的作用机制[J].新乡医学院学报,2020,37(1):026-29.[doi:10.7683/xxyxyxb.2020.01.007]
　PENG Shuangchun,CHEN Milian,OU Cehua.Mechanism of CD95 in atorvastatin against trigeminal neuralgia of rats[J].Journal of Xinxiang Medical University,2020,37(1):026-29.[doi:10.7683/xxyxyxb.2020.01.007]
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CD95在阿托伐他汀抗大鼠三叉神经痛中的作用机制
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
37
期数:
2020年1
页码:
026-29
栏目:
基础研究
出版日期:
2020-01-05

文章信息/Info

Title:
Mechanism of CD95 in atorvastatin against trigeminal neuralgia of rats
作者:
彭双春; 陈米炼; 欧册华
（西南医科大学附属医院疼痛科，四川　泸州　646000）
Author(s):
PENG Shuangchun; CHEN Milian; OU Cehua
(Department of Pain,the Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China)
关键词:
CD95; 阿托伐他汀; 三叉神经痛; 核因子-κB
Keywords:
CD95; atorvastain; trigeminal neuralgia; nuclear factor-κB
分类号:
R651.3；R965
DOI:
10.7683/xxyxyxb.2020.01.007
文献标志码:
A
摘要:
目的　探讨CD95在阿托伐他汀抗大鼠三叉神经痛中的作用机制。方法　雄性Sprague Dawley大鼠30只随机分为假手术组、三叉神经痛组和阿托伐他汀组，每组10只。三叉神经痛组和阿托伐他汀组大鼠结扎右侧眶下神经远端制备三叉神经痛模型，假手术组大鼠仅暴露右侧眶下神经，不结扎。造模后，阿托伐他汀组大鼠每天给予阿托伐他汀钙灌胃（10 mg·kg-1），共14 d；假手术组、三叉神经痛组大鼠每天给予等体积生理盐水灌胃，共14 d。分别于造模前1 d及造模后3、7、14 d检测各组大鼠机械痛阈。造模后第15天处死大鼠取前额叶皮质,采用Western blot检测各组大鼠前额叶皮质中CD95、核因子-κB（NF-κB）、p-NF-κB、白细胞介素（IL）-1β蛋白表达量。结果　造模前3组大鼠机械痛阈比较差异无统计学意义（P>0.05）。造模后3、7、14 d，三叉神经痛组和阿托伐他汀组大鼠机械痛阈低于假手术组（P<0.05）；造模后3 d，三叉神经痛组和阿托伐他汀组大鼠机械痛阈比较差异无统计学意义（P>0.05）；造模后7、14 d，阿托伐他汀组大鼠机械痛阈高于三叉神经痛组（P<0.05）。三叉神经痛组大鼠前额叶皮质中CD95、NF-κB、p-NF-κB 、IL-1β蛋白表达量高于假手术组（P<0.05）；阿托伐他汀组和假手术组大鼠前额叶皮质中CD95、NF-κB、p-NF-κB 、IL-1β蛋白表达量比较差异无统计学意义（P>0.05）；阿托伐他汀组大鼠前额叶皮质中CD95、NF-κB、p-NF-κB 、IL-1β蛋白表达量低于三叉神经痛组（P<0.05）。结论　阿托伐他汀可减轻三叉神经痛大鼠疼痛，其机制可能与抑制前额叶皮质中CD95、NF-κB、p-NF-κB 、IL-1β蛋白表达，从而抑制前额叶皮质神经炎症有关。
Abstract:
Objective　To investigate the mechanism of CD95 in atorvastatin against trigeminal neuralgia of rats.Methods　Thirty adult male Sprague Dawley rats were randomly divided into sham operation group,trigeminal neuralgia group and atorvastain group,with 10 rats in each group.The right infraorbital nerve of rats in trigeminal neuralgia group and atorvastain group was dissociated and ligated to prepare the trigeminal neuralgia model;the right infraorbital nerve of rats in sham operation group was dissociated without ligation.After modeling,the rats in atorvastain group were given atorvastatin calcium (10 mg·kg-1) by intragastric administration daily for 14 days,the rats in sham operation group and trigeminal neuralgia group were given equivalent volume of normal saline daily for 14 days.The mechanical pain threshold of all rats was detected at 1 day before modeling and 3,7,14 days after modeling.At the 15th day after modeling,all rats were sacrificed and the prefrontal cortex was reserved.The expressions of CD95,nuclear factor-κB（NF-κB）,p-NF-κB and interleukin（IL）-1β protein in the prefrontal cortex were detected by Western blot.Results　There was no significant difference in mechanical pain threshold among the three groups before modeling(P>0.05).At 3,7,14 days after modeling,the mechanical pain threshold of rats in trigeminal neuralgia group and atorvastain group were lower than those in the sham operation group(P<0.05);at 3 days after modeling,there was no significant difference in mechanical pain threshold of rats between trigeminal neuralgia group and atorvastain group(P>0.05);at 7,14 days after modeling,the mechanical pain threshold of rats in the atorvastain group was higher than that in the trigeminal neuralgia group(P<0.05).The expressions of CD95,NF-κB,p-NF-κB and IL-1β protein of rats in the trigeminal neuralgia group were higher than those in the sham operation group (P<0.05);there was no significant difference in the expressions of CD95,NF-κB,p-NF-κB and IL-1β protein between the sham operation group and atorvastain group(P>0.05);the expressions of CD95,NF-κB,p-NF-κB and IL-1β protein of rats in the atorvastain group were lower than those in the trigeminal neuralgia group (P<0.05).Conclusions　Atorvastatin can alleviate the pain of rats with trigeminal neuralgia,and its mechanism may be related to the inhibition of CD95,NF-κB,p-NF-κB,IL-1β expression in prefrontal cortex.

参考文献/References:

［1］　KOOPMAN J S,DIELEMAN J P,HUYGEN F J,et al.Incidence of facial pain in the general population［J］.Pain,2009,147(1/2/3):122-127.
［2］　DIETRICH P Y,WALKER P R,SAAS P.Death receptors on reactive astrocytes: a key role in the fine tuning of brain inflammation［J］.Neurology,2003,60(4):548-554.
［3］　CHU L W,CHEN J Y,WU P C,et al.Atorvastatin prevents neuroinflammation in chronic constriction injury rats through nuclear NF-κB downregulation in the dorsal root ganglion and spinal cord［J］.ACS Chem Neurosci,2015,6(6):889-898.
［4］　DING W,YOU Z,SHEN S,et al.An improved rodent model of trigeminal neuropathic pain by unilateral chronic constriction injury of distal infraorbital nerve［J］.J Pain,2017,18(8):899-907.
［5］　JI R R,CHAMESSIAN A,ZHANG Y Q.Pain regulation by non-neuronal cells and inflammation［J］.Science,2016,354(6312):572-577.
［6］　JI R R,NACKLEY A,HUH Y,et al.Neuroinflammation and central sensitization in chronic and widespread pain［J］.Anesthesiology,2018,129(2):343-366.
［7］　李永乐,李伟彦.他汀类药物抗炎机制及其在神经病理性疼痛中的应用［J］.医学研究生学报,2013,26(9):1001-1003.
［8］　CHOI C,BENVENISTE E N.Fas ligand/Fas system in the brain: regulator of immune and apoptotic responses［J］.Brain Res Brain Res Rev,2004,44(1):65-81.
［9］　王璐.三叉神经痛对大鼠神经退行性变的影响及CD95/CD95L通路在其中的作用机制研究［D］.泸州：西南医科大学,2018.
［10］　FIORE N T,AUSTIN P J.Peripheral nerve injury triggers neuroinflammation in the medial prefrontal cortex and ventral hippocampus in a subgroup of rats with coincident affective behavioural changes［J］.Neuroscience,2019,416:147-167.
［11］　BLANCO-COLIO L M,MARTIN-VENTURA J L,DE TERESA E,et al.Increased soluble Fas plasma levels in subjects at high cardiovascular risk:atorvastatin on inflammatory markers (AIM) study,a substudy of ACTFAST［J］.Arterioscler Thromb Vasc Biol,2007,27(1):168-174.
［12］　丘玥,陈唯韫,王之遥,等.辛伐他汀对大鼠神经病理性疼痛行为学和RhoA通路表达的影响［J］.中国疼痛医学杂志,2016,22(7):493-500.
［13］　PATHAK N N,BALAGANUR V,LINGARAJU M C,et al.Atorvastatin attenuates neuropathic pain in rat neuropathy model by down-regulating oxidative damage at peripheral,spinal and supraspinal levels［J］.Neurochem Int,2014,68:1-9.
［14］　PATHAK N N,BALAGANUR V,LINGARAJU M C,et al.Antihyperalgesic and anti-inflammatory effects of atorvastatin in chronic constriction injury-induced neuropathic pain in rats［J］.Inflammation,2013,36(6):1468-1478.

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更新日期/Last Update: 2020-01-05