[1]周　航,王慧敏,闫义涛,等.嗅觉障碍小鼠嗅上皮组织中Nogo受体及其配体Nogo-A的表达及意义[J].新乡医学院学报,2019,36(9):824-827.[doi:10.7683/xxyxyxb.2019.09.005]
　ZHOU Hang,WANG Hui-min,YAN Yi-tao,et al.Significance of the expression of Nogo receptor and its ligand Nogo-A in olfactory epithelium of mice with olfactory dysfunction[J].Journal of Xinxiang Medical University,2019,36(9):824-827.[doi:10.7683/xxyxyxb.2019.09.005]
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嗅觉障碍小鼠嗅上皮组织中Nogo受体及其配体Nogo-A的表达及意义
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
36
期数:
2019年9
页码:
824-827
栏目:
基础研究
出版日期:
2019-09-05

文章信息/Info

Title:
Significance of the expression of Nogo receptor and its ligand Nogo-A in olfactory epithelium of mice with olfactory dysfunction
作者:
周　航1; 王慧敏1; 闫义涛2; 卢振民1; 李　靖1
（1.新乡医学院第一附属医院耳鼻咽喉科，河南　卫辉　453100；2.新乡医学院第一附属医院眼科，河南　卫辉　453100）
Author(s):
ZHOU Hang1; WANG Hui-min1; YAN Yi-tao2; LU Zhen-min1; LI Jing1
（1.Department of Otorhinolaryngology，the First Affiliated Hospital of Xinxiang Medical University，Weihui 453100，Henan Province，China；2.Department of Ophthalmology，the First Affiliated Hospital of Xinxiang Medical University，Weihui 453100，Henan Province，China）
关键词:
Nogo受体; Nogo-A; 嗅觉功能障碍
Keywords:
Nogo receptor; Nogo-A; olfactory dysfunction
分类号:
R651.1+5
DOI:
10.7683/xxyxyxb.2019.09.005
文献标志码:
A
摘要:
目的　探讨Nogo受体（NgR）及其配体Nogo-A在嗅觉障碍小鼠嗅上皮组织中的表达及意义。方法　将100只BALB/C小鼠随机分为对照组（n=20）和模型组（n=80）。模型组小鼠给予体积分数0.7% Triton X-100 100 μL 双侧鼻腔注射建立嗅觉障碍模型，对照组小鼠给予等体积生理盐水。于造模后3、7、21、49 d采用埋藏食物颗粒实验检测2组小鼠觅食时间。取对照组及模型组小鼠造模后3、7、21、49 d嗅上皮组织，免疫组织化学法检测小鼠嗅上皮组织中NgR蛋白表达，反转录-聚合酶链反应检测小鼠嗅上皮组织中Nogo-A、NgR mRNA的表达，Western blot法及酶联免疫吸附试验分别检测嗅上皮组织中NgR蛋白、Nogo-A蛋白相对表达量。结果　模型组小鼠造模后3、7、21 d觅食时间长于对照组及造模后 49 d（P<0.05）。模型组小鼠造模后49 d觅食时间与对照组比较差异无统计学意义（P>0.05）。模型组小鼠造模后3、7、21、49 d嗅上皮组织中NgR阳性表达率显著高于对照组（P<0.05）。模型组小鼠造模后3、7、21、49 d 嗅上皮组织中NgR、Nogo-A mRNA及NgR、Nogo-A蛋白相对表达量均高于对照组（P<0.05）。结论　嗅觉障碍小鼠嗅上皮组织中NgR、Nogo-A表达上调，NgR与其配体Nogo-A相互作用可能参与了小鼠嗅觉障碍的形成。
Abstract:
Objective　To investigate the significance of the expression of Nogo receptor（NgR） and its ligand Nogo-A in olfactory epithelium of mice with olfactory dysfunction.Methods　One hundred BALB/C mice were randomly divided into control group （n=20） and model group （n=80）.The mice in the model group were given 100 μL 0.7% Triton X-100 bilateral nasal injection to establish the olfactory dysfunction model，while the mice in the control group were given equal volume of normal saline.The foraging time of mice in the two group was detected by buried food particles at 3，7，21 and 49 days after modeling.The olfactory epithelium tissue of mice in the control group and model group at 3,7,21,49 days after modeling was obtained，the expression of NgR protein in olfactory epithelium tissues of mice was detected by immunohistochemistry，the expression of Nogo-A and NgR mRNA in olfactory epithelium tissues was detected by reverse transcriptase-polymerase chain reaction，and the relative expression of NgR and Nogo-A protein in olfactory epithelium tissues was detected by Western blot and enzyme-linked immunosorbent assay.Results　The foraging time of mice in the model group at 3，7 and 21 days after modeling was longer than that in the control group and at 49 days after modeling in the model group （P<0.05）.There was no significant difference in the foraging time between the model group and the control group at 49 days after modeling （P>0.05）.The NgR positive expression rate in olfactory epithelium tissues of the model group was significantly higher than that in the control group （P<0.05）.The relative expression levels of NgR and Nogo-A mRNA，NgR and Nogo-A protein in olfactory epithelium tissues of mice in the model group were all higher than those in the control group （P<0.05）.Conclusion　The expression of NgR and Nogo-A is up-regulated in olfactory epithelium of mice with olfactory dysfunction.The interaction between NgR and its ligand Nogo-A is involved in the formation of olfactory dysfunction in mice.

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更新日期/Last Update: 2019-09-05