[1]郝海涛,姚东晓,赵洪洋.Nogo-A中不同功能片段对体外培养大鼠皮质神经元细胞内钙离子浓度的影响[J].新乡医学院学报,2016,33(4):272-274.[doi:10.7683/xxyxyxb.2016.04.006]
　HAO Hai-tao,YAO Dong-xiao,ZHAO Hong-yang.Effect of different functional fragments of Nogo-A on calcium concentration in rat corticalneurons cultured in vitro[J].Journal of Xinxiang Medical University,2016,33(4):272-274.[doi:10.7683/xxyxyxb.2016.04.006]
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Nogo-A中不同功能片段对体外培养大鼠皮质神经元细胞内钙离子浓度的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年4
页码:
272-274
栏目:
基础研究
出版日期:
2016-04-06

文章信息/Info

Title:
Effect of different functional fragments of Nogo-A on calcium concentration in rat corticalneurons cultured in vitro
作者:
郝海涛; 姚东晓; 赵洪洋
（华中科技大学同济医学院附属协和医院神经外科，湖北　武汉　430000）
Author(s):
HAO Hai-tao; YAO Dong-xiao; ZHAO Hong-yang
(Department of Neurosurgery，Union Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430000，Hubei Province,China)
关键词:
Nogo-A; 钙离子; 大鼠; 神经元; 激光共聚焦显微镜
Keywords:
Nogo-A; calcium; rat; neuron; laser scanning confocal microscope
分类号:
Q421
DOI:
10.7683/xxyxyxb.2016.04.006
文献标志码:
A
摘要:
目的　研究Nogo-A主要功能片段Nogo-66、amino-Nogo对大鼠皮质神经元细胞内钙离子浓度的影响。方法　体外原代培养并鉴定新生大鼠皮质神经元，将神经元按照干预药物不同分为对照组(给予Krebs-ringer液)、Nogo-66组、amino-Nogo组、amino-Nogo+尼莫地平组，以Fluo4/AM标记神经元细胞内钙离子，采用激光共聚焦显微镜技术检测药物干预1、3、5、7 min时各组神经元细胞内钙离子信号。结果　加入药物后,对照组和Nogo-66组神经元细胞内钙离子信号强度在各时间点未见明显变化（P>0.05），而amino-Nogo组和amino-Nogo+尼莫地平组钙离子信号持续升高（P<0.05）。组间比较结果显示，在药物干预1 min时，4组间的神经元细胞内钙离子信号比较差异无统计学意义（P>0.05）；在药物干预3、5、7 min时，Nogo-66组神经元细胞内钙离子信号与对照组比较差异无统计学意义（P<0.05），而amino-Nogo组和amino-Nogo+尼莫地平组神经元细胞内钙离子信号与对照组和Nogo-66组同时间点比较均升高，差异有统计学意义（P<0.05）。在药物干预3 min时，amino-Nogo组神经元细胞内钙离子信号与amino-Nogo+尼莫地平组比较差异无统计学意义（P>0.05）；在药物干预5、7 min时，amino-Nogo组神经元细胞内钙离子信号高于amino-Nogo+尼莫地平组，差异有统计学意义（P<0.05）。结论　amino-Nogo是Nogo-A中导致神经元细胞内钙离子浓度升高的责任功能片段。
Abstract:
Objective　To investigate the effect of major functional fragments,Nogo-66 and amino-Nogo of Nogo-A on the calcium concentration in rat cortical neurons cultured in vitro.Methods　Cortical neurons of new-born rats were cultured in vitro and then identified.The neurons were divided into four groups by different interference conditions：normal control group（krebs-ringer group），Nogo-66 group，amino-Nogo group and amino-Nogo+Nimodipine group.By using laser scanning confocal microscope，neuronal calcium signals marked by Fluo4/AM were tested at 1,3,5,7 min after interfered.Results　After drug administration，the calcium signals of control group and Nogo-66 group had no significant change at all time points（P>0.05）,the calcium signals of amino-Nogo group and amino-Nogo+Nimodipine group were remarkably increased(P<0.05).At the time point of 1 min，there was no significant difference in calcium signals among the four groups（P>0.05）;at the time points of 3,5,7 min,the calcium signals of Nogo-66 group had no significant change compared with control group(P>0.05),while the calcium signals of amino-Nogo group and amino-Nogo+Nimodipine group were significantly increased compared with control group and Nogo-66 group(P<0.05);at the time point of 3 min,there was no significant change in calcium signals between amino-Nogo group and amino-Nogo+Nimodipine group（P>0.05）,at the time point of 5,7 min,calcium signals of amino-Nogo group was remarkably increased compared with amino-Nogo+Nimodipine group(P<0.05).Conclusion　The functional fragment of Nogo-A which increases calcium concentration in cortical neurons of new-born rats is amino-Nogo.

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更新日期/Last Update: 2016-04-05