[1]成　力,陈文江,姚　峰,等.miRNA-505-3p在原发性高血压发病机制中的作用[J].新乡医学院学报,2018,35(11):965-970.[doi:10.7683/xxyxyxb.2018.11.005]
　CHENG Li,CHEN Wen-jiang,YAO Feng,et al.Role of miRNA-505-3p in the pathogenesis of essential hypertension[J].Journal of Xinxiang Medical University,2018,35(11):965-970.[doi:10.7683/xxyxyxb.2018.11.005]
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miRNA-505-3p在原发性高血压发病机制中的作用
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
35
期数:
2018年11
页码:
965-970
栏目:
基础研究
出版日期:
2018-11-05

文章信息/Info

Title:
Role of miRNA-505-3p in the pathogenesis of essential hypertension
作者:
成　力1; 陈文江2; 姚　峰3; 李小庆1
（1.陆军军医大学附属第二医院心血管内科，重庆　400037；2.重庆医科大学附属第二医院心血管内科，重庆　400010；3.广东医科大学附属医院心血管内科，广东　湛江　524000）
Author(s):
CHENG Li1; CHEN Wen-jiang2; YAO Feng3; LI Xiao-qing1
（1.Department of Vasculocardiology，the Second Affiliated Hospital of Army Medical University，Chongqing 400037，China；2.Department of Vasculocardiology，the Second Affiliated Hospital of Chongqing Medical University，Chongqing 400010，China；3.Department of Vasculocardiology，the Affiliated Hospital of Guangdong Medical University，Zhanjiang 524000，Guangdong Province，China）
关键词:
原发性高血压; miRNA-505-3p; 生物学标志物; 胰岛素样生长因子-1; 主动脉平滑肌细胞
Keywords:
essential hypertension; miRNA-505-3p; biomarkers; insulin-like growth factor-1; aortic smooth muscle cell
分类号:
R544.1
DOI:
10.7683/xxyxyxb.2018.11.005
文献标志码:
A
摘要:
目的　探讨miRNA-505-3p在原发性高血压（EH）发生机制中的作用。方法　选择2014年5～8月于陆军军医大学附属第二医院收治的EH患者和非EH患者各60例，采集患者静脉血，分离血浆，采用实时荧光定量聚合酶链反应（PCR）检测血浆中miRNA-505-3p相对表达量，采用酶联免疫吸附试验检测血浆中胰岛素样生长因子-1（IGF-1）水平。培养人主动脉平滑肌细胞（HASMC），当细胞融合度达到50%～70%时随机分为mimic组（转染miRNA-505-3p mimic序列）、mimic对照组（转染mimic对照序列）和空白对照组（不进行转染）；转染后继续培养72 h，然后收集细胞及细胞培养液。采用实时荧光定量PCR检测3组HASMC中miRNA-505-3p及IGF-1 mRNA表达，细胞计数试剂盒-8检测3组HASMC的活性，Western blot法检测3组HASMC中IGF-1蛋白表达。结果　EH患者与非EH患者血浆中miRNA-505-3p相对表达量分别为0.156 5±0.025 9、0.007 9±0.001 4，EH患者血浆中miRNA-505-3p相对表达量显著高于非EH患者（t=6.880，P<0.01）。EH患者和非EH患者血浆miRNA-505-3p的受试者工作特征曲线下面积为0.988 1，95%可信区间为0.972 4～0.999 0（P<0.01）。EH患者和非EH患者血浆中IGF-1水平分别为（1 338.03±96.92）、（620.90±23.19）ng·L-1，EH患者血浆中IGF-1水平显著高于非EH患者（t=7.101，P<0.01）。mimic组、mimic对照组和空白对照组HASMC活性分别为0.679 7±0.032 8、0.471 0±0.024 5、0.525 2±0.035 3；mimic组HASMC活性显著高于mimic对照组和空白对照组（t=6.203、4.610，P<0.05），mimic对照组与空白对照组HASMC活性比较差异无统计学意义（t=1.432，P>0.05）。mimic组、mimic对照组和空白对照组HASMC中miRNA-505-3p相对表达量分别为 0.693 3±0.022 9、0.020 6±0.000 8、0.023 8±0.001 1，mimic组HASMC中miRNA-505-3p相对表达量显著高于mimic对照组和空白对照组（t=7.991、5.840，P<0.01），mimic对照组与空白对照组HASMC中miRNA-505-3p相对表达量比较差异无统计学意义（t=0.323，P>0.05）。mimic组、mimic对照组和空白对照组HASMC中IGF-1 mRNA相对表达量分别为0.238 6±0.013 3、0.082 4±0.004 0、0.079 7±0.004 7，mimic组HASMC中IGF-1 mRNA相对表达量显著高于mimic对照组和空白对照组（t=16.903、18.240，P<0.01），mimic对照组与空白对照组HASMC中IGF-1 mRNA相对表达量比较差异无统计学意义（t=0.295，P>0.05）。mimic组、mimic对照组和空白对照组HASMC中IGF-1蛋白相对表达量分别为0.602 0±0.017 3、0.040 3±0.006 2、0.051 6±0.001 0；mimic组HASMC中IGF-1蛋白相对表达量显著高于mimic对照组和空白对照组（t=29.026、31.557，P<0.01），mimic对照组与空白对照组HASMC中IGF-1蛋白相对表达量比较差异无统计学意义（t=0.516，P>0.05）。结论　EH患者血浆miRNA-505-3p表达上调，miRNA-505-3p可能通过调控IGF-1表达而参与EH的发病，并可作为EH的生物学标志物。
Abstract:
Objective　To investigate the role of miRNA-505-3p in the pathogenesis of essential hypertension （EH）.Methods　Sixty EH patients and sixty non-EH patients were selected from May to August 2014 in the Second Affiliated Hospital of Army Medical University.The venous blood samples were collected，and the plasma was separated.The expression of miRNA-505-3p in plasma of EH patients and non-EH patients was detected by real-time fluorescence quantitative polymerase chain reaction （PCR）;the level of insulin-like growth factor-1 （IGF-1） in plasma was detected by enzyme linked immunosorbent assay.The human aortic smooth muscle cells （HASMC） were cultured，and the cells were randomly divided into mimic group （transfected with miRNA-505-3p mimic sequence），mimic-control group （transfected with mimic control sequence） and blank control group （not transfected） when the cell fusion reached 50%-70%.The cells were cultured for 72 hours after transfection，then the cells and cell culture medium were collected.The expression of miRNA-505-3p and IGF-1 mRNA in HASMC of the three groups was detected by real-time fluorescence quantitative PCR，the activity of HASMC was detected by cell counting kit-8，and the expression of IGF-1 protein in HASMC was detected by Western blot.Results　The relative expression of plasma miRNA-505-3p in EH patients and non-EH patients was （0.156 5±0.025 9） and （0.007 9±0.001 4），respectively.The relative expression of plasma miRNA-505-3p in EH patients was significantly higher than that in non-EH patients （t=6.880，P<0.01）.The area under the receiver operating characteristic curve of plasma miRNA-505-3p in EH patients and non-EH patients was 0.988 1，and the 95% confidence interval was 0.972 4-0.999 0 （P<0.01）.The plasma IGF-1 level in EH patients and non-EH patients was （1 338.03±96.92） and （620.90±23.19） ng·L-1，respectively.The plasma IGF-1 level in EH patients were significantly higher than that in non-EH patients （t=7.101，P<0.01）.The activity of HASMC in the mimic group，mimic-control group and blank control group was 0.679 7±0.032 8，0.471 0±0.024 5 and 0.525 2±0.035 3，respectively.The activity of HASMC in the mimic group was significantly higher than that in the mimic-control group and blank control group （t=6.203，4.610；P<0.05），but there was no significant difference in the activity of HASMC between the mimic-control group and blank control group （t=1.432，P>0.05）.The relative expression of miRNA-505-3p in HASMC of the mimic group，mimic-control group and blank control group was 0.693 3±0.022 9，0.020 6±0.000 8 and 0.023 8±0.001 1，respectively.The relative expression of miRNA-505-3p in HASMC of the mimic group was significantly higher than that of the mimic-control group and blank control group （t=7.991，5.840；P<0.01）.There was no significant difference in the relative expression of miRNA-505-3p in HASMC between the mimic-control group and blank control group （t=0.323，P>0.05）.The relative expression of IGF-1 mRNA in HASMC of the mimic group，mimic-control group and blank control group was 0.238 6±0.013 3，0.082 4±0.004 0 and 0.079 7±0.004 7，respectively.The relative expression of IGF-1 mRNA in HASMC of the mimic group was significantly higher than that of mimic-control group and blank control group （t=16.903，18.240；P<0.01）.There was no significant difference in the relative expression of IGF-1 mRNA between the mimic-control group and blank control group （t=0.295，P>0.05）.The relative expression of IGF-1 protein in HASMC of the mimic group，mimic-control group and blank control group was 0.602 0±0.017 3，0.040 3±0.006 2 and 0.051 6±0.001 0，respectively.The relative expression of IGF-1 protein in HASMC of the mimic group was significantly higher than that of mimic-control group and blank control group （t=29.026，31.557；P<0.01）.There was no significant difference in the relative expression of GF-1 protein between the mimic-control group and blank control group （t=0.516，P>0.05）.Conclusion　The expression of miRNAs-505-3p in plasma of EH patients is up-regulated.MiRNAs-505-3p may participate in the pathogenesis of EH by regulating the expression of IGF-1，and may be used as a biomarker of EH.

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更新日期/Last Update: 2018-11-05