[1]张　英,马瑞霞,杜艳云,等.MicroRNA-15b在子宫内膜癌组织中的表达及其对子宫内膜癌细胞增殖的影响[J].新乡医学院学报,2018,35(3):177-181.[doi:10.7683/xxyxyxb.2018.03.004]
　ZHANG Ying,MA Rui-xia,DU Yan-yun,et al.Expression of microRNA-15b in endometrial carcinoma and its effect on proliferation of endometrial carcinoma cells[J].Journal of Xinxiang Medical University,2018,35(3):177-181.[doi:10.7683/xxyxyxb.2018.03.004]
点击复制

MicroRNA-15b在子宫内膜癌组织中的表达及其对子宫内膜癌细胞增殖的影响
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
35
期数:
2018年3
页码:
177-181
栏目:
基础研究
出版日期:
2018-03-05

文章信息/Info

Title:
Expression of microRNA-15b in endometrial carcinoma and its effect on proliferation of endometrial carcinoma cells
作者:
张　英1; 马瑞霞1; 杜艳云2; 史亚男3
（1.新乡市中心医院妇瘤一科，河南　新乡　453000；2.河南省荣康医院妇产科，河南　新乡　453000；3.中山大学第一附属医院妇产科，广东　广州　510000）
Author(s):
ZHANG Ying1; MA Rui-xia1; DU Yan-yun2; SHI Ya-nan3
（1.Department of Gynecologic Oncology One Division，Xinxiang Central Hospital，Xinxiang 453000，Henan Province，China;2.Department of Obstetrics and Gynecology，Rongkang Hospital of Henan Province，Xinxiang 453000，Henan Province，China;3.Department of Obstetrics and Gynecology，the First Affiliated Hospital of Sun Yat-sen University，Guangzhou 510000，Guangdong Province，China）
关键词:
子宫内膜癌; microRNA-15b; 细胞增殖; 细胞凋亡
Keywords:
endometrial carcinoma; microRNA-15b; cell proliferation; cell apoptosis
分类号:
R737.33
DOI:
10.7683/xxyxyxb.2018.03.004
文献标志码:
A
摘要:
目的　探讨microRNA-15b（miR-15b）在子宫内膜癌组织中的表达及其对子宫内膜癌细胞增殖的影响。方法　选取2013年2月至2017年2月新乡市中心医院收治的87例子宫内膜癌患者手术切除的子宫内膜癌组织，另选取45例因子宫肌瘤行子宫全切术患者的正常子宫内膜组织作为对照，采用实时荧光定量聚合酶链反应（PCR）检测子宫内膜癌组织和正常子宫内膜组织中miR-15b的表达，并分析miR-15b表达与患者临床病理特征的关系。培养人子宫内膜癌HEC-1A细胞，随机分为miR-15b模拟物组、对照序列组和空白对照组，采用实时荧光定量PCR检测各组细胞中miR-15b表达，四甲基偶氮唑盐（MTT）法检测各组细胞增殖能力，流式细胞术检测各组细胞凋亡率和细胞周期变化。结果　子宫内膜癌组织和正常子宫内膜组织中miR-15b相对表达量分别为1.32±0.13、2.49±0.16，子宫内膜癌组织中miR-15b相对表达量低于正常子宫内膜组织（t=46.184，P<0.05）；子宫内膜癌组织中miR-15b相对表达量与国际妇产科联合会分期、肌层浸润深度和淋巴结转移有关（P<0.05），与年龄、组织学类型、分化程度、是否累及淋巴管和血管无关（P>0.05）。与空白对照组和对照序列组比较，miR-15b模拟物组人子宫内膜癌细胞中miR-15b相对表达量显著增加（P<0.05），培养24、48、72、96 h时细胞增殖能力均降低（P<0.05），细胞凋亡率升高（P<0.05），G0+G1期细胞比例显著增加（P<0.05），而G2+M期细胞比例减少（P<0.05）。空白对照组与对照序列组人子宫内膜癌细胞中miR-156相对表达量、培养12、24、48、72、96 h时细胞增殖能力、细胞凋亡率及G0+G期、S期和G2+M期细胞比例比较差异均无统计学意义（P>0.05）。结论　miR-15b在子宫内膜癌组织中呈低表达，上调子宫内膜癌细胞中miR-15b基因表达可抑制细胞增殖，其机制可能与阻滞细胞周期及促进细胞凋亡有关。
Abstract:
Objective　To investigate the expression of microRNA-15b（miR-15b） in endometrial carcinoma and its effect on proliferation of endometrial carcinoma cells.Methods　Eighty-seven cases of endometrial cancer tissues were selected as the research objects from patients with endometrial cancer undergoing endometrial cancer staging surgery in Xinxiang Central Hospital from February 2013 to February 2017.Another 45 cases of normal endometrial tissues from patients with uterine fibroids undergoing line hysterectomy were collected as the control.The expressions of miR-15b in endometrial carcinoma tissues and normal endometrial tissues were detected by real-time quantitative polymerase chain reaction（PCR） and relationship between the miR-15b expression and clinicopathological feature was analyzed.The HEC-1A cells were cultured and were randomly divided into miR-15b mimics group，control sequence group and blank control group.The expression of miR-15b in HEC-1A cells was detected by real-time quantitative PCR.Tetramethylazolazole blue （MTT） assay was used to detect the cell proliferation.Flow cytometry was used to detect the apoptotic rate and cell cycle.Results　The relative expression levels of miR-15b in endometrial carcinoma tissues and normal endometrial tissues were 1.32±0.13 and 2.49±0.16，respectively.The relative expression level of miR-15b in endometrial carcinoma tissues was lower than that in the normal endometrial tissues （t=46.184，P<0.05）.The relative expression level of miR-15b in endometrial carcinoma was correlated with International federation of gynecology and obstetrics staging，myometrial invasion and lymph node metastasis （P<0.05），but it was not correlated with age,histological type,differentiated degree,in filtrating lymph vessel and blood vessel or not.Compared with the blank control group and the control sequence group，the relative expression level of miR-15b in miR-15b mimics group was significantly increased （P<0.05），the cell proliferation abilities at cultured 24，48，72 and 96 h were decreased （P<0.05），while the apoptosis rate increased （P<0.05），the proportion of G0+G1 phase was significantly increased，while the proportion of G2+M phase was decreased （P<0.05）.There was no significant difference in the relative expression level of miR-156,the cell proliferation ability at cultured 12,24,48,72,96 h,the apoptosis rate,the proportion of G0+G1,S and G2+M phase between the blank control group and the control sequence group(P>0.05).Conclusion　miR-15b is low expression in endometrial carcinoma tissue.Up-regulation of miR-15b expression in endometrial carcinoma cells can inhibit cell proliferation，it may be related to block cell cycle to promote cell apoptosis.

参考文献/References:

［1］　SIMONY A，HANSEN E J，CHRISTENSEN S B，et al.Incidence of cancer in adolescent idiopathic scoliosis patients treated 25 years previously［J］.Eur Spine J，2016，25（10）:3366-3370.
［2］　ZANFAGNIN V，FERRERO A，BIGLIA N，et al.The role of surgery in recurrent endometrial cancer［J］.Expert Rev Anticancer Ther，2016，16（7）:741-750.
［3］　郝义，孙笑，刘玉秋，等.miRNA146a 在视网膜色素上皮细胞老化及老年性黄斑变性中的功能研究［J］.眼科新进展，2017，37（2）:117-121.
［4］　黄芳延，杨蕾，尤梁慧，等.miR-1908过表达的人脂源性多能干细胞基因表达谱分析［J］.中华实用儿科临床杂志，2016，31（7）:496-498.
［5］　季青山，俞茜，孙思勤，等.miR-34a/SIRT1在H2O2诱导人晶状体上皮细胞氧化应激中的表达［J］.眼科新进展，2017，37（8）：728-731.
［6］　汤有才，李恩耀，魏明，等.MicroRNA-146a对寻常型银屑病患儿血CD4+T淋巴细胞的调控作用［J］.中华实用儿科临床杂志，2016，31（17）:1332-1335.
［7］　LIU X，LIU X，WU Y，et al.MicroRNAs in biofluids are novel tools for bladder cancer screening［J］.Oncotarget，2017，8（19）:32370-32379.
［8］　符春艳.miR-93及miR-21在糖尿病视网膜病变患者中的表达及其临床价值［J］.眼科新进展，2017，37（12）:1161-1164.
［9］　VARAMO C，OCCELLI M，VIVENZA D，et al.miRNAs and their role in the correlation between schizophrenia and cancer （review）［J］.Mol Med Rep，2016，14（6）:4942-4946.
［10］　丁肖媛，蔡红，王嵩，等.玉屏风散对支气管哮喘小鼠微小 RNA 表达的影响［J］.中华实用儿科临床杂志，2016，31（4）:284-287.
［11］　李娜，曹娟，郁继国，等.miR26a对葡萄膜黑色素瘤细胞增殖、凋亡、迁移和侵袭的影响及机制研究［J］.眼科新进展，2017，37（7） :619-622.
［12］　马岳，伍俊，廖旺，等.miRNA-100通过抑制羧基末端区域小磷酸化酶样蛋白表达促进人白血病细胞 HL-60增殖［J］.中华实用儿科临床杂志，2016，31（3）:185-189.
［13］　ZHAO C，WANG G，ZHU Y，et al.Aberrant regulation of miR-15b in human malignant tumors and its effects on the hallmarks of cancer［J］.Tumour Biol，2016，37（1）:177-183.
［14］　刘薇，马静茹，张春雨.低频脉冲电刺激干预对宫颈癌根治术后尿潴留的影响［J］.新乡医学院学报，2017，34（4）:327-329.
［15］　SURI V，ARORA A.Management of endometrial cancer:a review［J］.Rev Recent Clin Trials，2015，10（4）:309-316.
［16］　BOGANI G，DOWDY S C，CLIBY W A，et al.Incisional recurrences after endometrial cancer surgery［J］.Anticancer Res，2015，35（11）:6097-6104.
［17］　周胤健，杜英，梅丽娜，等.子宫内膜癌microRNA表达谱的初步研究［J］.中华实验和临床病毒学杂志，2014，28（4）:280-282.
［18］　ZHANG W L，ZHANG J H，WU X Z，et al.miR-15b promotes epithelial-mesenchymal transition by inhibiting SMURF2 in pancreatic cancer［J］.Int J Oncol，2015，47（3）:1043-1053.
［19］　WANG X，GUO H，YAO B，et al.miR-15b inhibits cancer-initiating cell phenotypes and chemoresistance of cisplatin by targeting TRIM14 in oral tongue squamous cell cancer［J］.Oncol Rep，2017，37（5）:2720-2726.
［20］　JI W B，LIU X，LUO Y，et al.High expression of miR-15b predicts poor prognosis for hepatocellular carcinoma after curative hepatectomy［J］.Oncol Rep，2016，36（4）:1901-1908.

相似文献/References:

[1]陈彩霞,赵孟军,李国芸.子宫内膜癌160例临床和手术病理分期比较[J].新乡医学院学报,2005,22(06):582.
　CHEN Caixia,ZI-UkO Mengjun,LI Guoyun.Comparison of clinical stage and operative pathological stage in 1 60 patients with endometrial cancer[J].Journal of Xinxiang Medical University,2005,22(3):582.
[2]周开梅,袁森辅,吴　晶,等.人附睾蛋白4和糖类抗原125在子宫内膜癌组织中的表达[J].新乡医学院学报,2018,35(3):219.[doi:10.7683/xxyxyxb.2018.03.015]
　ZHOU Kai-mei,YUAN Sen-fu,WU Jing,et al.Expression of human epididymis protein 4 and carbohydrate antigen 125 in endometrial carcinoma tissues[J].Journal of Xinxiang Medical University,2018,35(3):219.[doi:10.7683/xxyxyxb.2018.03.015]
[3]琚　莹,杨　君.MicroRNA-182和Ki-67在Ⅰ型和Ⅱ型子宫内膜癌组织中的表达[J].新乡医学院学报,2020,37(1):057.[doi:10.7683/xxyxyxb.2020.01.014]
　JU Ying,YANG Jun.Expression of microRNA-182 and Ki-67 in type Ⅰ and type Ⅱ endometrial carcinoma tissues[J].Journal of Xinxiang Medical University,2020,37(3):057.[doi:10.7683/xxyxyxb.2020.01.014]
[4]赵彩琴,赵宝祥.新辅助放射治疗和化学治疗联合手术治疗晚期子宫内膜癌效果观察[J].新乡医学院学报,2019,36(1):074.[doi:10.7683/xxyxyxb.2019.01.016]
　ZHAO Cai-qin,ZHAO Bao-xiang.Effect of neoadjuvant radiotherapy and chemotherapy combined with surgery in the treatment of advanced endometrial carcinoma[J].Journal of Xinxiang Medical University,2019,36(3):074.[doi:10.7683/xxyxyxb.2019.01.016]
[5]白云云,王世进,李　赟.血清人附睾蛋白4和糖类抗原125联合检测对子宫内膜癌淋巴结转移的诊断价值[J].新乡医学院学报,2021,38(8):725.[doi:10.7683/xxyxyxb.2021.08.005]
　BAI Yunyun,WANG Shijin,LI Yun.Diagnostic value of combined detection of serum human epididymal protein 4 and carbohydrate antigen 125 for lymph node metastasis in endometrial cancer[J].Journal of Xinxiang Medical University,2021,38(3):725.[doi:10.7683/xxyxyxb.2021.08.005]
[6]豆秋彦,冯　文,李春霖.脂肪因子chemerin在女性常见恶性肿瘤中的作用研究进展[J].新乡医学院学报,2020,37(12):1195.[doi:10.7683/xxyxyxb.2020.12.021]
[7]寇建芳,孟跃进.宫腔镜检查联合血清人附睾分泌蛋白及糖类抗原和miR-21早期诊断子宫内膜癌的临床价值[J].新乡医学院学报,2019,36(2):185.[doi:10.7683/xxyxyxb.2019.02.022]
　KOU Jian-fang,MENG Yue-jin.Clinical value of hysteroscopy combined with serum human epididymis protein 4,carbohydrate antigen 125 and miR-21 in early diagnosis of endometrial carcinoma[J].Journal of Xinxiang Medical University,2019,36(3):185.[doi:10.7683/xxyxyxb.2019.02.022]
[8]郭会敏,范毛川,华方方,等.脂联素对子宫内膜癌Ishikawa细胞增殖及侵袭和转移能力的影响[J].新乡医学院学报,2018,35(7):563.[doi:10.7683/xxyxyxb.2018.07.004]
　GUO Hui-min,FAN Mao-chuan,HUA Fang-fang,et al.Effects of adiponectin on invasion and metastasis of endometrial carcinoma Ishikawa cells[J].Journal of Xinxiang Medical University,2018,35(3):563.[doi:10.7683/xxyxyxb.2018.07.004]
[9]徐　岚.大蒜素促进人子宫内膜癌细胞株HEC-1细胞凋亡的细胞外信号调节激酶信号通路研究[J].新乡医学院学报,2016,33(1):020.[doi:10.7683/xxyxyxb.2016.01.006]
　XU Lan.Study on the extracellular signal-regulated kinase signal pathway of allicin promoting apoptosis in human endometrial cancer HEC-1 cell strain[J].Journal of Xinxiang Medical University,2016,33(3):020.[doi:10.7683/xxyxyxb.2016.01.006]
[10]朱春霞,孟　勇,王秀虹.血清人附睾分泌蛋白4、糖类抗原125和脂联素在子宫内膜癌淋巴结转移诊断中的价值[J].新乡医学院学报,2016,33(5):403.[doi:10.7683/xxyxyxb.2016.05.016]
　ZHU Chun-xia,MENG Yong,WANG Xiu-hong.Value of serum human epididymis protein 4，carbohydrate antigen 125 and adiponectin in the diagnosis of lymphatic metastasis in patients with endometrial carcinoma[J].Journal of Xinxiang Medical University,2016,33(3):403.[doi:10.7683/xxyxyxb.2016.05.016]

更新日期/Last Update: 2018-03-05