[1]宋宝杰,吕敬媛,邢天容,等.丝氨酸蛋白酶A3和转化生长因子-β在子宫内膜异位症患者中的表达及临床意义[J].新乡医学院学报,2016,33(3):204-207.[doi:10.7683/xxyxyxb.2016.03.012]
　SONG Bao-jie,LYU Jing-yuan,XING Tian-rong,et al.Value of expression of high-temperature requirement factor A3,transforming growth factor-β in the endometrial tissue and serum of patient with endometriosis[J].Journal of Xinxiang Medical University,2016,33(3):204-207.[doi:10.7683/xxyxyxb.2016.03.012]
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丝氨酸蛋白酶A3和转化生长因子-β在子宫内膜异位症患者中的表达及临床意义
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年3
页码:
204-207
栏目:
临床研究
出版日期:
2016-03-05

文章信息/Info

Title:
Value of expression of high-temperature requirement factor A3,transforming growth factor-β in the endometrial tissue and serum of patient with endometriosis
作者:
宋宝杰1; 吕敬媛2; 邢天容2; 陈　丽2
（1.佳木斯大学，黑龙江　佳木斯　154002；2.佳木斯大学附属第一医院妇产科，黑龙江　佳木斯　154007）
Author(s):
SONG Bao-jie1; LYU Jing-yuan2; XING Tian-rong2; CHEN Li2
(1.Jiamusi University,Jiamusi 154002,Heilongjiang Province,China;2.Department of Gynaecology and Obstetrics,the First Affiliated Hospital of Jiamusi University,Jiamusi 154007,Heilongjiang Province,China)
关键词:
丝氨酸蛋白酶A3; 转化生长因子-β; 免疫组织化学; 酶联免疫吸附试验; 子宫内膜异位症
Keywords:
high-temperature requirement factor A3; transforming growth factor-β; immunohistochemtistry; enzyme-linked immunosorbent assay; endometriosis
分类号:
R711.71
DOI:
10.7683/xxyxyxb.2016.03.012
文献标志码:
A
摘要:
目的　探讨子宫内膜异位症(EMT)患者内膜组织中丝氨酸蛋白酶A3（HtrA3）、转化生长因子-β(TGF-β)及血清中HtrA3水平变化及其临床意义。方法　选取2014年9月至2015年6月于佳木斯大学附属第一医院确诊的EMT患者30例（观察组）和卵巢囊肿患者30例（对照组）,观察组患者取其异位内膜、在位内膜组织及外周血，对照组患者取其正常子宫内膜及外周血，采用免疫组织化学方法分别检测HtrA3、TGF-β在异位内膜、在位内膜及正常子宫内膜组织中的表达；应用酶联免疫吸附法（ELISA）测定HtrA3在2组患者血清中的水平。结果　观察组患者异位内膜、在位内膜HtrA3表达水平显著低于对照组正常子宫内膜（P<0.01），且观察组患者异位内膜HtrA3表达水平显著低于在位内膜（P<0.01）。观察组患者异位内膜、在位内膜TGF-β表达水平显著高于对照组正常子宫内膜（P<0.01），且观察组患者异位内膜TGF-β表达水平显著高于在位内膜（P<0.01）。HtrA3与TGF-β水平在异位内膜组织中的表达呈负相关（r=-0.485，P<0.01）。与对照组比较，观察组患者术前、术后第2、7天血清HtrA3水平显著降低（P<0.01）；观察组患者术前、术后第2天外周血中HtrA3水平均显著低于术后第7天（P<0.01）；观察组患者术前血清中HtrA3水平与术后第2天比较差异无统计学意义（P>0.05）。结论　EMT患者内膜组织中HtrA3、TGF-β的表达异常，可能在EMT发病的初始阶段发挥作用，血清中低水平表达的HtrA3可能有助于临床诊断和预后评估。
Abstract:
Objective　To investigate the value of expression of high-temperature requirement factor A3 (HtrA3),transforming growth factor-β (TGF-β) in endometrial tissue and the level of serum HtrA3 of patient with endometriosis.Methods　Thirty patients with endometriosis (observation group) and thirty patients with leiomyoma (control group) were selected in the First Affiliated Hospital of Jiamusi University from September 2014 to June 2015.The expression of HtrA3,TGF-β in ectopic,eutopic and normal endometrial tissue were assayed by immunohistochemistry.The level of HtrA3 in the peripheral blood was measured by enzyme-linked immunosorbent assay (ELISA).Results　The expression of HtrA3 in ectopic,eutopic endometrial tissue of patients in observation group was significantly lower than that in control group (P<0.01),besides,the expression of HtrA3 in ectopic tissue was markedly lower than eutopic tissue (P<0.01).The level of TGF-β in ectopic,eutopic endometrial tissue of patients in observation group was significantly higher than that in control group(P<0.01),and the level of TGF-β in ectopic endometrial tissue was significantly higher than eutopic endometrial tissue (P<0.01).The expression of HtrA3 and TGF-β in ectopic endometrial tissue was negatively correlated (r=-0.485,P<0.01).The concentration of serum HtrA3 in before operation,2 days and 7 days after operation of patients in observation group were significantly lower than control group;the concentration of serum HtrA3 in before operation,2 days after operation in patients of obseration group were significantly lower than 7 days after operation (P<0.01).There was no significant difference of serum HtrA3 between the before operation and 2 days after operation (P>0.05).Conclusion　The data show that the abnormal expression of HtrA3 and TGF-β in endometrial tissue of patients with endometriosis might play a role in the initial phase of pathogenesis of endometriosis,the low level of serum HtrA3 may contribute to the clinical diagnosis and follow-up.

参考文献/References:

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更新日期/Last Update: 2016-03-05