[1]孟　勇,汪　涛.同源转录因子2、环氧合酶-2及核因子-κB对胃癌患者病情进展的预测价值[J].新乡医学院学报,2016,33(1):037-40.[doi:10.7683/xxyxyxb.2016.01.011]
　MENG Yong,WANG Tao.Value of cognate transcription factor 2,cyclooxygenase-2 and nuclear factor-κB in predicting the progression of gastric cancer[J].Journal of Xinxiang Medical University,2016,33(1):037-40.[doi:10.7683/xxyxyxb.2016.01.011]
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同源转录因子2、环氧合酶-2及核因子-κB对胃癌患者病情进展的预测价值
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
33
期数:
2016年1
页码:
037-40
栏目:
临床研究
出版日期:
2016-01-05

文章信息/Info

Title:
Value of cognate transcription factor 2,cyclooxygenase-2 and nuclear factor-κB in predicting the progression of gastric cancer
作者:
孟　勇; 汪　涛
（咸宁市中心医院胃肠科，湖北　咸宁　437100）
Author(s):
MENG Yong; WANG Tao
（Department of Gastroenterology,the Central Hospital of Xianning City,Xianning 437100,Hubei Province,China）
关键词:
同源转录因子2; 环氧合酶-2; 核因子-κB; 胃癌
Keywords:
homologous transcription factor 2; cyclooxygenase-2; nuclear factor-κB; gastric cancer
分类号:
R735.2
DOI:
10.7683/xxyxyxb.2016.01.011
文献标志码:
A
摘要:
目的　探讨同源转录因子2（CDX2）、环氧合酶-2（COX-2）、核因子-κB（NF-κB）对胃癌患者病情进展的预测价值及其相互关系。方法　应用免疫组织化学法和反转录聚合酶链反应检测30例正常胃黏膜组织、42例胃癌癌前病变组织及34例胃癌组织中CDX2、COX-2、NF-κB蛋白及其mRNA的表达水平。结果　胃癌组织和癌前病变组织中CDX2、COX-2、NF-κB mRNA的表达显著高于正常胃黏膜组织（P<0.05），胃癌组织中COX-2、NF-κB mRNA的表达显著高于癌前病变组织（P<0.05），胃癌组织中CDX2 mRNA的表达显著低于癌前病变组织（P<0.05）。胃癌组织和癌前病变组织中CDX2、COX-2、NF-κB蛋白阳性表达率显著高于正常胃黏膜组织（P<0.05），胃癌组织中COX-2、NF-κB蛋白阳性表达率显著高于癌前病变组织（P<0.05），胃癌组织中CDX2蛋白阳性表达率显著低于癌前病变组织（P<0.05）。CDX2表达与胃癌临床分期、分化程度有关（P<0.05），COX-2和NF-κB表达与临床分期、分化程度、肿瘤直径及淋巴结转移有关（P<0.05）。CDX2与COX-2、NF-κB表达呈负相关（P<0.05），COX-2与NF-κB表达呈正相关（P<0.05）。结论　CDX2表达下调及COX-2、NF-κB表达上调可能与胃癌的发生发展、浸润及淋巴结转移有关，通过对三者表达及关系进行分析有助于了解胃癌患者的病情进展及预后。
Abstract:
Objective　To investigate the value of homologous transcription factor 2 (CDX2),cyclooxygenase-2 (COX-2) and nuclear factor-κB (NF-κB) in predicting the progression of gastric cancer and their relationships.Methods　The expressions of CDX2,COX-2,NF-κB protein and mRNA in 30 cases of normal gastric mucosa tissue,34 cases of precancerous tissue and 42 cases of gastric cancer tissue were detected by immunohistochemical method and reverse transcription-polymerase chain reaction.Results　The expressions of CDX2,COX-2 and NF-κB mRNA in gastric cancer tissue and precancerous tissue were significantly higher than those in normal gastric mucosa tissue(P<0.05).The expressions of COX-2 and NF-κB mRNA in gastric cancer tissue were significantly higher than those in precancerous tissue(P<0.05).The expression of CDX2 mRNA in gastric cancer tissue was significantly lower than that in precancerous tissue(P<0.05).The expressions of CDX2,COX-2 and NF-κB protein in gastric cancer tissue and precancerous tissue were significantly higher than those in normal gastric mucosa tissue(P<0.05).The expressions of COX-2 and NF-κB protein in gastric cancer tissue were significantly higher than those in precancerous tissue(P<0.05).The expression of CDX2 protein in gastric cancer tissue was significantly lower than that in precancerous tissue(P<0.05).CDX2 expression was related to clinical stage and differentiation degree of gastric cancer(P<0.05).The expression of COX-2 and NF-κB was related to clinical stage,differentiation degree,tumor diameter and lymph node metastasis(P<0.05).CDX2 expression was negatively correlated with COX-2 and NF-κB(P<0.05).COX-2 expression was positively correlated with NF-κB(P<0.05).Conclusion　The down-regulation of CDX2 expression and the up-regulation of COX-2 and NF-κB expression may be related to the occurrence,development,invasion and lymph node metastasis of gastric cancer.It is helpful to evaluate the progress and prognosis of gastric cancer patients by analyzing the expression and relationship of them.

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更新日期/Last Update: 2016-01-05