[1]杨鑫,王晓蕾,杨孟丽,等.星状神经节阻滞对大鼠心肌缺血再灌注损伤的影响[J].新乡医学院学报,2023,40(8):707-711.[doi:10.7683/xxyxyxb.2023.08.002]
　YANG Xin,WANG Xiaolei,YANG Mengli,et al.Effect of stellate ganglion block on myocardial ischemia-reperfusion injury in rats[J].Journal of Xinxiang Medical University,2023,40(8):707-711.[doi:10.7683/xxyxyxb.2023.08.002]
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星状神经节阻滞对大鼠心肌缺血再灌注损伤的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
40卷
期数:
2023年8
页码:
707-711
栏目:
基础研究
出版日期:
2023-08-05

文章信息/Info

Title:
Effect of stellate ganglion block on myocardial ischemia-reperfusion injury in rats
文章编号:
20230802
作者:
杨鑫1; 王晓蕾1; 杨孟丽1; 张红伟1; 樊腾1; 马闻苛1; 杨明月1; 高宁宁1; 殷婕1; 郭自伟1; 张雪莹1; 王玉淼1; 李丽2; 岳修勤1
（1.新乡医学院第一附属医院麻醉科，河南　卫辉　453100；2.首都医科大学附属北京友谊医院麻醉科，北京　100050）
Author(s):
YANG Xin1; WANG Xiaolei1; YANG Mengli1; ZHANG Hongwei1; FAN Teng1; MA Wenke1; YANG Mingyue1; GAO Ningning1; YIN Jie1; GUO Ziwei1; ZHANG Xueying1; WANG Yumiao1; LI Li2; YUE Xiuqin1
关键词:
星状神经节阻滞; 心肌缺血再灌注; 心肌酶; 氧化应激
Keywords:
(1.Department of Anesthesiology; the First Affiliated Hospital of Xinxiang Medical University; Weihui 453100; Henan Province; China; 2.Department of Anesthesiology; Beijing Friendship Hospital; Capital Medical University; Beijing 100050; China)
分类号:
R541.4
DOI:
10.7683/xxyxyxb.2023.08.002
文献标志码:
A
摘要:
目的　探讨星状神经节阻滞对大鼠心肌缺血再灌注损伤的影响。
方法　将30只8周龄健康Sprague Dawley雄性大鼠按照随机数字表法分为对照组、缺血再灌注组和星状神经节阻滞组，每组10只。缺血再灌注组、星状神经节阻滞组大鼠采用冠状动脉左前降支结扎法制备心肌缺血再灌注模型，对照组大鼠用丝线穿过冠状动脉左前降支但不结扎。星状神经节阻滞组大鼠再灌注即刻使用罗哌卡因行星状神经节阻滞。记录3组大鼠手术前（T0）、结扎30 min（T1）、再灌注后30 min（T2）、再灌注后60 min（T3）、再灌注后120 min（T4）的心率（HR）和平均动脉压（MAP）；再灌注结束后，取各组大鼠颈动脉血5 mL，采用酶联免疫吸附试验（ELISA）法检测血清心肌肌钙蛋白I（cTnI）、肌酸激酶同工酶（CK-MB）以及乳酸脱氢酶（LDH）水平；取3组大鼠缺血区心肌组织，采用ELISA法检测心肌组织中丙二醛（MDA）及超氧化物歧化酶（SOD）表达。
结果　T0时3组大鼠的MAP、HR比较差异均无统计学意义（F=2.863、2.949，P>0.05）。T1、T2、T3、T4时3组大鼠的MAP比较差异均有统计学意义（F=94.628、76.462、86.647、121.432，P<0.05）；T1、T2、T3、T4时,缺血再灌注组和星状神经节阻滞组大鼠的MAP均显著低于对照组（P<0.05）；T2、T3、T4时星状神经节阻滞组大鼠的MAP均显著高于缺血再灌注组（P<0.05）。 T1、T2、T3、T4时3组大鼠的HR比较差异均有统计学意义（F=126.462、168.564、98.678、128.751，P<0.05）；T1、T2、T3、T4时,缺血再灌注组和星状神经节阻滞组大鼠的HR均显著高于对照组（P<0.05）；T2、T3、T4时星状神经节阻滞组大鼠的HR均显著低于缺血再灌注组（P<0.05）。缺血再灌注组和星状神经节阻滞组大鼠血清cTnI、LDH、CK-MB水平均显著高于对照组(P<0.05)；星状神经节阻滞组大鼠血清cTnI、LDH、CK-MB水平均显著低于缺血再灌注组(P<0.05)。缺血再灌注组和星状神经节阻滞组大鼠心肌组织中MDA水平显著高于对照组，SOD活性显著低于对照组(P<0.05)；星状神经节阻滞组大鼠心肌组织中MDA水平显著低于缺血再灌注组，SOD活性显著高于缺血再灌注组(P<0.05)。
结论　星状神经节阻滞能够改善缺血再灌注大鼠的心肌灌注，通过减轻氧化应激而减轻心肌缺血再灌注损伤。
Abstract:
Objective　To investigate the effect of stellate ganglion block on myocardial ischemia-reperfusion injury in rats.
Methods　Thirty healthy 8-week-old Sprague Dawley male rats were divided into control group,ischemia-reperfusion group and stellate ganglion block group according to random number table method,with 10 rats in each group.The rats in the ischemia-reperfusion group and stellate ganglion block group prepared myocardial ischemia-reperfusion model by ligating the left anterior descending branch of the coronary artery,the rats in the control group were threaded through the left anterior descending branch of the coronary artery without ligation.The rats in the stellate ganglion block group were immediately performed with stellate ganglion block with ropivacaine.The heart rate (HR) and mean arterial pressure (MAP) of rats in the three groups were recorded before operation(T0), 30 min after ligation (T1),30 min after reperfusion (T2),60 min after reperfusion (T3),and 120 min after reperfusion (T4).After reperfusion,5 mL of carotid artery blood of rats in each group was taken,and the levels of serum cardiac troponin I (cTnI),creatine kinase isoenzyme (CK-MB) and lactate dehydrogenase (LDH) were detected by enzyme-linked immunosorbent assay(ELISA);the myocardial tissue in ischemic area of rats in the three groups was taken,and the expressions of malondialdehyde (MDA) and superoxide dismutase (SOD) in myocardial tissue were detected by ELISA.
Results　There were no significant differences in MAP and HR at T0 among the three groups(F=2.863,2.949;P>0.05).There were statistically significant differences in MAP of rats among the three groups at T1,T2,T3 and T4 (F=94.628,76.462,86.647,121.432;P<0.05);at T1,T2,T3 and T4,the MAP of rats in the ischemia-reperfusion group and stellate ganglion block group was significantly lower than that in the control group (P<0.05);at T2,T3 and T4,the MAP of rats in the stellate ganglion block group was significantly higher than that in the ischemia-reperfusion group (P<0.05).There were statistically significant differences in HR of rats among the three groups at T1,T2,T3 and T4 (F=126.462,168.564,98.678,128.751;P<0.05);at T1,T2,T3 and T4,the HR of rats in the ischemia-reperfusion group and stellate ganglion block group was significantly higher than that in the control group (P<0.05);at T2,T3 and T4,the HR of rats in the stellate ganglion block group was significantly lower than that in the ischemia-reperfusion group (P<0.05).The levels of serum cTnI,LDH and CK-MB of rats in the ischemia-reperfusion group and the stellate ganglion block group were significantly higher than those in the control group (P<0.05).The levels of serum cTnI,LDH and CK-MB of rats in the stellate ganglion block group were significantly lower than those in the ischemia-reperfusion group (P<0.05).The level of MDA in myocardial tissue of rats in the ischemia-reperfusion group and stellate ganglion block group was significantly higher than that in the control group,and the SOD activity was significantly lower than that in the control group (P<0.05);the level of MDA in myocardial tissue of rats in the stellate ganglion block group was significantly lower than that in the ischemia-reperfusion group,and the SOD activity was significantly higher than that in the ischemia-reperfusion group (P<0.05).
Conclusion　Stellate ganglion block can improve myocardial perfusion in ischemia-reperfusion rats,and alleviate myocardial ischemia-reperfusion injury by reducing oxidative stress.

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更新日期/Last Update: 2023-08-14