[1]杜鹏程,韩　璠,谷九莲.溃疡性结肠炎患者血清单核细胞趋化蛋白-1、高迁移率族蛋白B1、肿瘤坏死因子-α、白细胞介素-6和白细胞介素-10水平与肠道菌群的相关性[J].新乡医学院学报,2021,38(4):370-373.[doi:10.7683/xxyxyxb.2021.04.016]
　DU Pengcheng,HAN Fan,GU Jiulian.Correlation between serum monocyte chemoattractant protein-1,high mobility group protein B1,tumor necrosis factor-α,interleukin-6,interleukin-10 levels and intestinal flora in patients with ulcera-tive colitis[J].Journal of Xinxiang Medical University,2021,38(4):370-373.[doi:10.7683/xxyxyxb.2021.04.016]
点击复制

溃疡性结肠炎患者血清单核细胞趋化蛋白-1、高迁移率族蛋白B1、肿瘤坏死因子-α、白细胞介素-6和白细胞介素-10水平与肠道菌群的相关性
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
38
期数:
2021年4
页码:
370-373
栏目:
临床研究
出版日期:
2021-04-05

文章信息/Info

Title:
Correlation between serum monocyte chemoattractant protein-1,high mobility group protein B1,tumor necrosis factor-α,interleukin-6,interleukin-10 levels and intestinal flora in patients with ulcera-tive colitis
作者:
杜鹏程; 韩　璠; 谷九莲
（新乡医学院第三附属医院消化科，河南　新乡　453003）
Author(s):
DU Pengcheng; HAN Fan; GU Jiulian
(Department of Gastroenterology,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003,Henan Province,China)
关键词:
溃疡性结肠炎; 肠道菌群; 单核细胞趋化蛋白-1; 高迁移率族蛋白B1; 炎症因子
Keywords:
ulcerative colitisintestinal floramonocyte chemoattractant protein-1high mobility group protein B1inflammatory factor
分类号:
R574.62
DOI:
10.7683/xxyxyxb.2021.04.016
文献标志码:
A
摘要:
目的　探讨溃疡性结肠炎（UC）患者血清单核细胞趋化蛋白-1（MCP-1）、高迁移率族蛋白B1（HMGB1）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-10（IL-10）水平与肠道菌群的相关性。方法　选择2018年1月至2019年12月新乡医学院第三附属医院收治的100例UC患者为研究对象，其中急性期45例（急性期组），缓解期55例（缓解期组）；另选择同期体检健康者50人作为对照组。应用微生物鉴定系统检测3组受试者肠道内双歧杆菌、乳酸杆菌、大肠埃希菌、肠球菌、拟杆菌的菌群数量，采用酶联免疫吸附试验检测血清MCP-1、HMGB1、TNF-α、IL-6、IL-10水平，并分析血清MCP-1、HMGB1、TNF-α、IL-6、IL-10水平与UC患者肠道菌群的相关性。结果　3组受试者肠道内双歧杆菌、乳酸杆菌、大肠埃希菌、肠球菌、拟杆菌的菌群数量比较差异有统计学意义（P<0.05）。急性期组患者肠道内双歧杆菌、乳酸杆菌数量低于缓解期组和对照组（>P<0.05），缓解期组患者肠道内双歧杆菌、乳酸杆菌数量低于对照组（>P<0.05）。急性期组患者肠道内大肠埃希菌、肠球菌、拟杆菌数量显著高于缓解期组和对照组（>P<0.05），缓解期组患者肠道内大肠埃希菌、肠球菌、拟杆菌数量显著高于对照组（>P<0.05）。3组受试者血清mcp-1、hmgb1、tnf-α、il-6、il-10水平比较差异有统计学意义（>P<0.05）。急性期组患者血清mcp-1、hmgb1、tnf-α、il-6水平显著高于缓解期组和对照组（>P<0.05），缓解期组患者血清mcp-1、hmgb1、tnf-α、il-6水平显著高于对照组（>P<0.05）。急性期组患者血清il-10水平显著低于缓解期组和对照组（>P<0.05），缓解期组患者血清il-10水平显著低于对照组（>P<0.05）。uc患者血清mcp-1、hmgb1、tnf-α、il-6水平与肠道内乳酸杆菌、双歧杆菌数量呈显著负相关（>P<0.05），与大肠埃希菌、肠球菌、拟杆菌数量呈显著正相关（>P<0.05）。uc患者血清il-10水平与肠道内双歧杆菌、乳酸杆菌数量呈显著正相关（>P<0.05），与大肠埃希菌、肠球菌、拟杆菌数量呈显著负相关（>P<0.05）。>结论　UC患者肠道菌群分布失衡，血清MCP-1、HMGB1、TNF-α、IL-6、IL-10水平与肠道菌群数量存在相关性。undefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefined
Abstract:
Objective　To investigate the relationship between the levels of serum monocyte chemoattractant protein-1 (MCP-1),high mobility group protein B1 (HMGB1),tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6),interleukin-10 (IL-10) and intestinal flora in patients with ulcerative colitis (UC).Methods　　A total of 100 patients with UC admitted to the Third Affiliated Hospital of Xinxiang Medical University from January 2018 to December 2019 were selected as the research subjects,including 45 cases in acute stage (acute stage group) and 55 cases in remission stage (remission stage group).Another 50 healthy people in the same period were selected as the control group.The number of intestinal flora including Bifidobacteria,Lactobacillus,Escherichia coli,Enterococcus and Bacteroides of the subjects in the three groups were detected by microbiological identification system.The levels of serum MCP-1,HMGB1,TNF-α,IL-6 and IL-10 were detected by enzyme linked immunosorbent assay.The correlation between the levels of serum MCP-1,HMGB1,TNF-α,IL-6,IL-10 and intestinal flora in UC patients was analyzed.Results　There were significant differences in the number of Bifidobacterium,Lactobacillus,Escherichia coli,Enterococcus and Bacteroides in the intestinal tract among the three groups (P<0.05).The number of Bifidobacteria and Lactobacilli in intestinal tract of patients in the acute stage group was lower than that in the remission stage group and the control group (P<0.05),and the number of Bifidobacteria and Lactobacilli in intestinal tract of patients in the remission stage group was lower than that in the control group (P<0.05).The number of Escherichia coli,Enterococcus and Bacteroides in intestinal tract of patients in the acute stage group was significantly higher than that in the remission stage group and the control group (P<0.05),and the number of Escherichia coli,Enterococcus and Bacteroides in intestinal tract of patients in the remission stage group was significantly higher than that in the control group (P<0.05).There were significant differences in serum MCP-1,HMGB1,TNF-α,IL-6 and IL-10 levels among the three groups (P<0.05).The levels of serum MCP-1,HMGB1,TNF-α and IL-6 in the acute stage group were significantly higher than those in the remission stage group and the control group (P<0.05),and the levels of serum MCP-1,HMGB1,TNF-α and IL-6 in the remission stage group were significantly higher than those in the control group (P<0.05).The level of serum IL-10 in the acute stage group was significantly lower than that in the remission stage group and the control group (P<0.05),and the level of serum IL-10 in the remission stage group was significantly lower than that in the control group (P<0.05).The levels of serum MCP-1,HMGB1,TNF-α and IL-6 were negatively correlated with the number of Lactobacillus and Bifidobacterium in intestinal tract of UC patients (P<0.05),and they were positively correlated with the number of Escherichia coli,Enterococcus and Bacteroides (P<0.05).The level of serum IL-10 was positively correlated with the number of Bifidobacteria and Lactobacillus in intestine tract in UC patients (P<0.05),and it was negatively correlated with the number of Escherichia coli,Enterococcus and Bacteroides (P<0.05).Conclusion　The distribution of intestinal flora is unbalanced in UC patients.The levels of serum MCP-1,HMGB1,TNF-α,IL-6 and IL-10 are correlated with the number of intestinal flora.

参考文献/References:

［1］　DAHAL L N,GADD A,EDWARDS A D,et al.UC-1V150,a potent TLR7 agonist capable of activating macrophages and potentiating mAb-mediated target cell deletion［J］.Scand J Immunol,2018,87(6):e12666.
［2］　杜小东，罗利飞.UC患者肠道菌群和肠黏膜屏障的变化及益生菌的干预作用［J］.中国微生态学杂志，2019，31（2）：193-196.
［3］　甄建华,黄光瑞.溃疡性结肠炎病因和发病机制的现代医学研究进展［J］.世界华人消化杂志，2019，27（4）：245-251.
［4］　胡景岚.ω-3多不饱和脂肪酸和美沙拉嗪联合治疗对UC患者血清辅助性T细胞1及辅助性T细胞2细胞因子水平的影响［J］.新乡医学院学报，2019，36（1）：67-70.
［5］　中华中医药学会脾胃病分会.溃疡性结肠炎中医诊疗专家共识意见［J］.中华中医药杂志，2017，32（8）：3585-3589.
［6］　冯媛，刘欣，史海涛，等.幽门螺杆菌感染对UC患者炎性细胞因子水平和肠道菌群含量的影响［J］.世界临床药物，2019，40（5）：334-339.
［7］　章科清，江琴，张海兵.溃疡性结肠炎患者粪菌移植后胃肠道功能及肠道菌群的影响分析［J］.世界华人消化杂志，2019，27（1）：68-72.
［8］　马思彦，董丽娜，王俊平.溃疡性结肠炎肠道菌群及其代谢产物的研究进展［J］.中国微生态学杂志，2020，32（7）：853-857.
［9］　张永利，申妮.溃疡性结肠炎患者肠道菌群的变化特征［J］.河北医药，2018，40（7）：1054-1057.
［10］　李琳，钟青.溃疡性结肠炎患者肠道菌群变化与细胞因子、TLRs分子表达的相关性研究［J］.传染病信息，2017，30（6）：361-364.
［11］　杜小东，罗利飞.溃疡性结肠炎患者肠道菌群和肠黏膜屏障的变化及益生菌的干预作用［J］.中国微生态学杂志，2019，31（2）：193-196.
［12］　王家丰，闫守泉，江连英，等.基于二代测序的HMGB1基因SNP发掘及其与UC风险关联分析［J］.广东医学，2019，40（10）：1356-1361.
［13］　刘永洁，席源，席佳.单核细胞趋化蛋白-1、高迁移率族蛋白B1与溃疡性结肠炎患者肠道菌群变化的相关性［J］.中国微生态学杂志，2019，31（8）：885-888.

相似文献/References:

[1]侯再恩.肠炎平治疗溃疡性结肠炎58例[J].新乡医学院学报,2003,20(05):376.
[2]侯再恩.肠炎平治疗溃疡性结肠炎58 例[J].新乡医学院学报,2003,20(05):376.
[3]张小毅,刘瑞丽,韩效林,等.愈疡液治疗大鼠溃疡性结肠炎的实验研究[J].新乡医学院学报,2003,20(01):021.
[4]张小毅,刘瑞丽,韩效林,等.愈疡液治疗大鼠溃疡性结肠炎的实验研究[J].新乡医学院学报,2003,20(01):021.
[5]柯贤胜.美沙拉嗪对溃疡性结肠炎患者血清白细胞介素-6、白细胞介素-8及肿瘤坏死因子-α的影响 [J].新乡医学院学报,2012,29(12):950.
[6]张　伟,杨银利,张　林,等.青柏溃结汤灌肠对溃疡性结肠炎大肠湿热证患者结肠黏膜愈合及血清炎性因子水平的影响[J].新乡医学院学报,2018,35(6):474.[doi:10.7683/xxyxyxb.2018.06.006]
　ZHANG Wei,YANG Yin-li,ZHANG Lin,et al.Effect of Qingbaikuijietang enema on colonic mucosa healing and serum inflammatory factors in patients with ulcerative colitis and large intestine damp-heat syndrome[J].Journal of Xinxiang Medical University,2018,35(4):474.[doi:10.7683/xxyxyxb.2018.06.006]
[7]王　超,袁　宇.冠状动脉粥样硬化性心脏病患者肠道菌群丰度与血脂和血尿酸水平的相关性[J].新乡医学院学报,2019,36(7):623.[doi:10.7683/xxyxyxb.2019.07.006]
　WANG Chao,YUAN Yu.Correlation between gut flora abundance and blood lipid,blood uric acid levels in patients with coronary atherosclerotic heart disease[J].Journal of Xinxiang Medical University,2019,36(4):623.[doi:10.7683/xxyxyxb.2019.07.006]
[8]何　艳,金孝岠.围手术期患者肠道菌群失调相关因素及其与术后并发症的关系研究进展[J].新乡医学院学报,2021,38(8):790.[doi:10.7683/xxyxyxb.2021.08.018]
[9]石　翠,袁　宇.冠状动脉性心脏病患者肠道菌群数量与血脂及同型半胱氨酸水平的相关性[J].新乡医学院学报,2021,38(1):044.[doi:10.7683/xxyxyxb.2021.01.009]
　SHI Cui,YUAN Yu.Correlation between the amount of the intestinal flora and blood lipid,homocysteine levels in patients with coronary heart disease[J].Journal of Xinxiang Medical University,2021,38(4):044.[doi:10.7683/xxyxyxb.2021.01.009]
[10]于佳卉,毛光兰,王庆志,等.沉默信号调节蛋白6在溃疡性结肠炎小鼠结肠组织中的表达及意义[J].新乡医学院学报,2021,38(9):806.[doi:10.7683/xxyxyxb.2021.09.002]
　YU Jiahui,MAO Guanglan,WANG Qingzhi,et al.Expression and role of silent information regulator 6 in colon tissues of mice with ulcerative colitis[J].Journal of Xinxiang Medical University,2021,38(4):806.[doi:10.7683/xxyxyxb.2021.09.002]
[11]朱佳丽,鲁素彩.小檗碱治疗溃疡性结肠炎研究进展[J].新乡医学院学报,2022,39(2):187.[doi:10.7683/xxyxyxb.2022.02.018]
　ZHU Jiali,LU Sucai.Research progress of berberine in the treatment of ulcerative colitis[J].Journal of Xinxiang Medical University,2022,39(4):187.[doi:10.7683/xxyxyxb.2022.02.018]
[12]朱瑜,郑志,朱绍辉,等.干酪乳杆菌-山药复合发酵剂对溃疡性结肠炎小鼠肠道菌群的调节及其抗炎作用[J].新乡医学院学报,2023,40(5):401.[doi:10.7683/xxyxyxb.2023.05.001]
　ZHU Yu,ZHENG Zhi,ZHU Shaohui,et al.Regulation effect of Lactobacillus casei-yam compound starter on intestinal flora and anti-inflammation in mice with ulcerative colitis[J].Journal of Xinxiang Medical University,2023,40(4):401.[doi:10.7683/xxyxyxb.2023.05.001]

更新日期/Last Update: 2021-04-05