[1]杜鹏程,韩 璠,谷九莲.溃疡性结肠炎患者血清单核细胞趋化蛋白-1、高迁移率族蛋白B1、肿瘤坏死因子-α、白细胞介素-6和白细胞介素-10水平与肠道菌群的相关性[J].新乡医学院学报,2021,38(4):370-373.[doi:10.7683/xxyxyxb.2021.04.016]
 DU Pengcheng,HAN Fan,GU Jiulian.Correlation between serum monocyte chemoattractant protein-1,high mobility group protein B1,tumor necrosis factor-α,interleukin-6,interleukin-10 levels and intestinal flora in patients with ulcera-tive colitis[J].Journal of Xinxiang Medical University,2021,38(4):370-373.[doi:10.7683/xxyxyxb.2021.04.016]
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溃疡性结肠炎患者血清单核细胞趋化蛋白-1、高迁移率族蛋白B1、肿瘤坏死因子-α、白细胞介素-6和白细胞介素-10水平与肠道菌群的相关性
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
38
期数:
2021年4
页码:
370-373
栏目:
临床研究
出版日期:
2021-04-05

文章信息/Info

Title:
Correlation between serum monocyte chemoattractant protein-1,high mobility group protein B1,tumor necrosis factor-α,interleukin-6,interleukin-10 levels and intestinal flora in patients with ulcera-tive colitis
作者:
杜鹏程韩 璠谷九莲
(新乡医学院第三附属医院消化科,河南 新乡 453003)
Author(s):
DU PengchengHAN FanGU Jiulian
(Department of Gastroenterology,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003,Henan Province,China)
关键词:
溃疡性结肠炎肠道菌群单核细胞趋化蛋白-1高迁移率族蛋白B1炎症因子
Keywords:
ulcerative colitisintestinal floramonocyte chemoattractant protein-1high mobility group protein B1inflammatory factor
分类号:
R574.62
DOI:
10.7683/xxyxyxb.2021.04.016
文献标志码:
A
摘要:
目的 探讨溃疡性结肠炎(UC)患者血清单核细胞趋化蛋白-1(MCP-1)、高迁移率族蛋白B1(HMGB1)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和白细胞介素-10(IL-10)水平与肠道菌群的相关性。方法 选择2018年1月至2019年12月新乡医学院第三附属医院收治的100例UC患者为研究对象,其中急性期45例(急性期组),缓解期55例(缓解期组);另选择同期体检健康者50人作为对照组。应用微生物鉴定系统检测3组受试者肠道内双歧杆菌、乳酸杆菌、大肠埃希菌、肠球菌、拟杆菌的菌群数量,采用酶联免疫吸附试验检测血清MCP-1、HMGB1、TNF-α、IL-6、IL-10水平,并分析血清MCP-1、HMGB1、TNF-α、IL-6、IL-10水平与UC患者肠道菌群的相关性。结果 3组受试者肠道内双歧杆菌、乳酸杆菌、大肠埃希菌、肠球菌、拟杆菌的菌群数量比较差异有统计学意义(P<0.05)。急性期组患者肠道内双歧杆菌、乳酸杆菌数量低于缓解期组和对照组(>P<0.05),缓解期组患者肠道内双歧杆菌、乳酸杆菌数量低于对照组(>P<0.05)。急性期组患者肠道内大肠埃希菌、肠球菌、拟杆菌数量显著高于缓解期组和对照组(>P<0.05),缓解期组患者肠道内大肠埃希菌、肠球菌、拟杆菌数量显著高于对照组(>P<0.05)。3组受试者血清mcp-1、hmgb1、tnf-α、il-6、il-10水平比较差异有统计学意义(>P<0.05)。急性期组患者血清mcp-1、hmgb1、tnf-α、il-6水平显著高于缓解期组和对照组(>P<0.05),缓解期组患者血清mcp-1、hmgb1、tnf-α、il-6水平显著高于对照组(>P<0.05)。急性期组患者血清il-10水平显著低于缓解期组和对照组(>P<0.05),缓解期组患者血清il-10水平显著低于对照组(>P<0.05)。uc患者血清mcp-1、hmgb1、tnf-α、il-6水平与肠道内乳酸杆菌、双歧杆菌数量呈显著负相关(>P<0.05),与大肠埃希菌、肠球菌、拟杆菌数量呈显著正相关(>P<0.05)。uc患者血清il-10水平与肠道内双歧杆菌、乳酸杆菌数量呈显著正相关(>P<0.05),与大肠埃希菌、肠球菌、拟杆菌数量呈显著负相关(>P<0.05)。>结论 UC患者肠道菌群分布失衡,血清MCP-1、HMGB1、TNF-α、IL-6、IL-10水平与肠道菌群数量存在相关性。undefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefinedundefined
Abstract:
Objective To investigate the relationship between the levels of serum monocyte chemoattractant protein-1 (MCP-1),high mobility group protein B1 (HMGB1),tumor necrosis factor-α (TNF-α),interleukin-6 (IL-6),interleukin-10 (IL-10) and intestinal flora in patients with ulcerative colitis (UC).Methods  A total of 100 patients with UC admitted to the Third Affiliated Hospital of Xinxiang Medical University from January 2018 to December 2019 were selected as the research subjects,including 45 cases in acute stage (acute stage group) and 55 cases in remission stage (remission stage group).Another 50 healthy people in the same period were selected as the control group.The number of intestinal flora including Bifidobacteria,Lactobacillus,Escherichia coli,Enterococcus and Bacteroides of the subjects in the three groups were detected by microbiological identification system.The levels of serum MCP-1,HMGB1,TNF-α,IL-6 and IL-10 were detected by enzyme linked immunosorbent assay.The correlation between the levels of serum MCP-1,HMGB1,TNF-α,IL-6,IL-10 and intestinal flora in UC patients was analyzed.Results There were significant differences in the number of Bifidobacterium,Lactobacillus,Escherichia coli,Enterococcus and Bacteroides in the intestinal tract among the three groups (P<0.05).The number of Bifidobacteria and Lactobacilli in intestinal tract of patients in the acute stage group was lower than that in the remission stage group and the control group (P<0.05),and the number of Bifidobacteria and Lactobacilli in intestinal tract of patients in the remission stage group was lower than that in the control group (P<0.05).The number of Escherichia coli,Enterococcus and Bacteroides in intestinal tract of patients in the acute stage group was significantly higher than that in the remission stage group and the control group (P<0.05),and the number of Escherichia coli,Enterococcus and Bacteroides in intestinal tract of patients in the remission stage group was significantly higher than that in the control group (P<0.05).There were significant differences in serum MCP-1,HMGB1,TNF-α,IL-6 and IL-10 levels among the three groups (P<0.05).The levels of serum MCP-1,HMGB1,TNF-α and IL-6 in the acute stage group were significantly higher than those in the remission stage group and the control group (P<0.05),and the levels of serum MCP-1,HMGB1,TNF-α and IL-6 in the remission stage group were significantly higher than those in the control group (P<0.05).The level of serum IL-10 in the acute stage group was significantly lower than that in the remission stage group and the control group (P<0.05),and the level of serum IL-10 in the remission stage group was significantly lower than that in the control group (P<0.05).The levels of serum MCP-1,HMGB1,TNF-α and IL-6 were negatively correlated with the number of Lactobacillus and Bifidobacterium in intestinal tract of UC patients (P<0.05),and they were positively correlated with the number of Escherichia coli,Enterococcus and Bacteroides (P<0.05).The level of serum IL-10 was positively correlated with the number of Bifidobacteria and Lactobacillus in intestine tract in UC patients (P<0.05),and it was negatively correlated with the number of Escherichia coli,Enterococcus and Bacteroides (P<0.05).Conclusion The distribution of intestinal flora is unbalanced in UC patients.The levels of serum MCP-1,HMGB1,TNF-α,IL-6 and IL-10 are correlated with the number of intestinal flora.

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更新日期/Last Update: 2021-04-05