[1]唐江萍,李　芳,程明军,等.绝经后女性盆底脏器脱垂患者阴道前壁组织中层粘连蛋白、胶原蛋白及雌激素受体的表达[J].新乡医学院学报,2020,37(12):1134-1137.[doi:10.7683/xxyxyxb.2020.12.007]
　TANG Jiangping,LI Fang,CHENG Mingjun,et al.Expression of laminin,collagen and estrogen receptor in the anterior vaginal wall tissues of post-menopallsal female patients with pelvic organ prolapse[J].Journal of Xinxiang Medical University,2020,37(12):1134-1137.[doi:10.7683/xxyxyxb.2020.12.007]
点击复制

绝经后女性盆底脏器脱垂患者阴道前壁组织中层粘连蛋白、胶原蛋白及雌激素受体的表达
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
37
期数:
2020年12
页码:
1134-1137
栏目:
临床研究
出版日期:
2020-12-05

文章信息/Info

Title:
Expression of laminin,collagen and estrogen receptor in the anterior vaginal wall tissues of post-menopallsal female patients with pelvic organ prolapse
作者:
唐江萍1; 李　芳1; 程明军1; 朱梅娟2; 蔡徐山3; 齐结华3; 曹云桂1
（1.上海市嘉定区妇幼保健院妇科，上海　201821；2.上海市嘉定区妇幼保健院病理科，上海　201821；3.上海市嘉定区妇幼保健院检验科，上海　201821）
Author(s):
TANG Jiangping1; LI Fang1; CHENG Mingjun1; ZHU Meijuan2; CAI Xushan3; QI Jiehua3; CAO Yungui1
（1.Department of Gynaecology,Materal and Child Health Care Hospital of Jiading District of Shanghai,Shanghai 201821,China；2.Department of Pathology,Materal and Child Health Care Hospital of Jiading District of Shanghai,Shanghai 201821,China；3.Department of Clinical Laboratory,Materal and Child Health Care Hospital of Jiading District of Shanghai,Shanghai 201821,China）
关键词:
盆底脏器脱垂; 层粘连蛋白; 胶原蛋白; 雌激素受体
Keywords:
pelvic organ prolapse; laminin; collagen; estrogen receptor
分类号:
R713.4
DOI:
10.7683/xxyxyxb.2020.12.007
文献标志码:
A
摘要:
目的　研究绝经后女性盆底脏器脱垂患者阴道前壁组织中层粘连蛋白（LN）、胶原蛋白及雌激素受体（ER）的表达。方法　选择2016年1月至2018年12月于上海市嘉定区妇幼保健院因绝经后盆底脏器脱垂行阴式全子宫切除术+阴道壁修补术的40例患者作为观察组；另选择同期因其他子宫良性病变行阴式子宫全切术的40例患者作为对照组。术前采用酶联免疫吸附试验检测2组患者血清LN水平；术中取2组患者阴道前壁组织，采用荧光定量聚合酶链反应法检测LN mRNA表达，采用免疫组织化学法检测LN、胶原蛋白Ⅰ、胶原蛋白Ⅲ及ER表达。结果　术前，观察组与对照组患者血清LN水平比较差异无统计学意义（P>0.05）。观察组患者阴道前壁组织中LN mRNA的相对表达量显著低于对照组（P<0.05）。观察组和对照组患者阴道前壁组织中LN阳性表达率分别为12.5%（5/40）、27.5%（11/40），观察组患者阴道前壁组织中LN阳性表达率显著低于对照组（χ2=45.262，P<0.01）。观察组患者阴道前壁组织中胶原蛋白Ⅰ、胶原蛋白Ⅲ及ER表达评分均显著低于对照组（P<0.05）。结论　LN、胶原蛋白Ⅰ、胶原蛋白Ⅲ及ER在绝经后女性盆底脏器脱垂患者阴道前壁组织中的表达降低，推测绝经后女性盆底脏器脱垂与以上因素有关。
Abstract:
Objective　To investigate the expression of laminin（LN）,collagen and estrogen receptor(ER) in the anterior vaginal wall tissues of postmenopausal female patients with pelvic organ prolapse.Methods　Forty postmenopausal female patients with pelvic organ prolapse who underwent transvaginal hysterectomy and repair of vaginal wall in the Maternal and Child Health Care Hospital of Jiading District of shanghai from January 2016 to December 2018 were selected as the observation group.Another 40 patients who underwent transvaginal hysterectomy due to other kind of benign uterine lesions were selected as the control group in the same period.Serum LN levels of patients in the two groups were measured by enzyme linked immunosorbent assay before operation.During the operation,the anterior vaginal wall tissues of patients in the two groups were taken and the expression of LN mRNA was detected by fluorescence quantitative polymerase chain reaction;the expressions of LN,collagen Ⅰ,collagen Ⅲ and ER were detected by immunohistochemistry.Results　There was no significant difference in the serum level of LN between the observation group and the control group before surgery（P>0.05）.The relative expression of LN mRNA in the anterior vagina wall tissues of patients in the observation group was significantly lower than that in the control group(P<0.05).The positive expression rates of LN in the anterior vagina wall tissues of patients in the observation group and the control group were 12.5% (5/40) and 27.5% (11/40),respectively;the positive expression rate of LN in the anterior vagina wall tissues of patients in the observation group was significantly lower than that in the control group(P<0.05).The expression scores of collagen Ⅰ,collagen Ⅲ and ER in the anterior vaginal wall tissues of patients in the observation group were significantly lower than those in the control group(P<0.05).Conclusion　The expressions of LN,collagen Ⅰ,collagen Ⅲ and ER in the anterior vaginal wall tissues of postmenopausal women with pelvic floor prolapse are lower.It may be the etiological factors of postmenopausal women with pelvic floor prolapse.

参考文献/References:

［1］　ABHYANKAR P,UNY I,SEMPLE K,et al.Women′s experiences of receiving care for pelvic organ prolapse:a qualitative study［J］.BMC Womens Health,2019,19(1):45.
［2］　KASSA D W,FEREDE Y G,ADVOLODKINA P.Validation of the pelvic organ prolapse simple screening inventory (POPSSI) in a population of Ethiopian women［J］.BMC Womens Health,2019,19(1):52.
［3］　BARBER M D,MAHER C.Epidemiology and outcome assessment［J］.Int Urogynecol J,2013,24(11):1783-1790.
［4］　陈璐璐,赵冰,郑雪芹,等.产后早期盆腔脏器脱垂的危险因素［J］.新乡医学院学报,2018,35(4):282-284.
［5］　常涛,王莉娜,任艺婷,等.绝经后女性盆底脏器脱垂患者阴道前壁及主骶韧带中转化生长因子-β3及层粘连蛋白mRNA的表达［J］.中国药物与临床,2013,18(6):699-702.
［6］　边中睿.特发性肺纤维化患者Ⅲ型前胶原和层粘连蛋白水平变化及其临床意义［J］.吉林医学,2013,34(9):1635-1636.
［7］　赵君利,王淑玉,郑漪霖,等.层粘连蛋白及其受体与子宫内膜癌关系的初探［J］.现代妇产科进展,2000,9(2):101-103.
［8］　马喆,杨媛媛,王慧军,等.绝经后阴道前壁膨出患者阴道组织中胶原蛋白与ERα表达的相关性分析［J］.广西医科大学学报,2017,34(9):1335-1337.
［9］　顾红艳,葛娟,何鲜,等.联合检测Ⅲ型前胶原、层粘连蛋白、Ⅳ型胶原及透明质酸在间质性肺疾病诊断中的临床意义［J］.东南大学学报(医学版),2017,26(6):938-941.
［10］　GOPALAKRISHNA R,GUNDIMEDA U,ZHOU S,et al.Laminin-1 induces endocytosis of 67 KDa laminin receptor and protects neuroscreen-1 cells against death induced by serum withdrawal ［J］.Biochem Biophys Res Commun,2018,495(1):230-237.
［11］　江海燕.血清铁蛋白、转铁蛋白、层粘连蛋白水平与肝硬化Child-Pugh分级的相关性分析［J］.标记免疫分析与临床,2019,26(2):285-289.
［12］　NAKAD B,FARES F,AZZAM N,et al.Estrogen receptor and laminin genetic polymorphism among women with pelvic organ prolapse［J］.Taiwan J Obstet Gynecol,2017,56(6):750-754.
［13］　康乐,陈丽,李彩霞,等.盆底器官脱垂患者主要支持韧带中雌激素受体GPR30表达的研究［J］.上海交通大学学报(医学版),2017,37(10):1376-1379.
［14］　李岱娟，岳良，孙永红，等.层粘连蛋白与Ⅳ型胶原在宫颈原位癌及微小浸润性癌鉴别诊断中的应用［J］.实用妇科内分泌杂志,2015,2(6):84-86.
［15］　EID S,IWANAGA J,OSKOUIAN R J,et al.Comprehensive review of the cardinal ligament［J］.Cureus,2018,10(6):e2846.

相似文献/References:

[1]李　青,白宏英,徐志秀,等.乌司他丁对脑缺血再灌注大鼠脑组织中基质金属蛋白酶-9活性和细胞外基质表达的影响[J].新乡医学院学报,2020,37(10):912.[doi:10.7683/xxyxyxb.2020.10.003]
　LI Qing,BAI Hongying,XU Zhixiu,et al.Effect of ulinastatin on the activity of matrix metalloproteinase-9 and the expression of extracellular matrix in brain tissues of rats with cerebral ischemia-reperfusion[J].Journal of Xinxiang Medical University,2020,37(12):912.[doi:10.7683/xxyxyxb.2020.10.003]

更新日期/Last Update: 2020-12-05