[1]吴宙光,王　斌,刘　冬,等.环状RNA 42398 调控转化生长因子-β1/Smad信号通路在大鼠胆道闭锁肝纤维化中的作用机制[J].新乡医学院学报,2020,37(11):1013-1017.[doi:10.7683/xxyxyxb.2020.11.003]
　WU Zhouguang,WANG Bin,LIU Dong,et al.Mechanism of role of circular RNA 42398 regulating the transforming growth factor-β1/smad signaling pathway in liver fibrosis of rats with biliary atresia[J].Journal of Xinxiang Medical University,2020,37(11):1013-1017.[doi:10.7683/xxyxyxb.2020.11.003]
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环状RNA 42398 调控转化生长因子-β1/Smad信号通路在大鼠胆道闭锁肝纤维化中的作用机制
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
37
期数:
2020年11
页码:
1013-1017
栏目:
基础研究
出版日期:
2020-11-05

文章信息/Info

Title:
Mechanism of role of circular RNA 42398 regulating the transforming growth factor-β1/smad signaling pathway in liver fibrosis of rats with biliary atresia
作者:
吴宙光; 王　斌; 刘　冬; 罗　珍; 郑佳辰; 李一帆; 孙　荣
（深圳市儿童医院普外科，广东　深圳　518026）
Author(s):
WU Zhouguang; WANG Bin; LIU Dong; LUO Zhen; ZHENG Jiachen; LI Yifan; SUN Rong
(Department of General Surgery,Shenzhen Children′s Hospital,Shenzhen 518026，Guangdong Province，China)
关键词:
环状 RNA 42398; 转化生长因子-β1/Smad信号通路; 胆道闭锁; 肝纤维化
Keywords:
circular RNA 42398; transforming growth factor-β1/Smad signaling pathway; biliary atresia; liver fibrosis
分类号:
R575.2
DOI:
10.7683/xxyxyxb.2020.11.003
文献标志码:
A
摘要:
目的　探讨环状RNA(circRNA) 42398调控转化生长因子-β1(TGF-β1)/Smad信号通路在大鼠胆道闭锁肝纤维化中的作用机制。方法　取大鼠肝星状细胞株HSC-T6构建慢病毒稳定感染的细胞系，根据转染RNA的不同将细胞分为阴性对照组、circRNA过表达组和circRNA敲低组。阴性对照组细胞转染阴性对照序列，circRNA过表达组细胞转染circRNA过表达序列，circRNA敲低组细胞转染circRNA低表达序列。应用细胞计数试剂盒-8检测各组细胞活性,采用实量定量聚合酶链式反应检测各组细胞中TGF-β1、Smad2和Smad3 mRNA的表达,采用Western blot法检测各组细胞中TGF-β1、Smad2、Smad3蛋白的表达。结果　各组细胞活性比较差异均无统计学意义（P>0.05）。circRNA过表达组与阴性对照组细胞中TGF-β1、Smad2、Smad3 mRNA和蛋白的相对表达量比较差异无统计学意义（P>0.05）。circRNA敲低组细胞中TGF-β1、Smad2、Smad3 mRNA和蛋白的相对表达量均显著高于阴性对照组（P<0.05）。结论　在大鼠细胞水平，circRNA 42398 可通过调控TGF-β1/Smad信号通路而抑制肝星状细胞活化，从而抑制胆道闭锁肝纤维化，这为胆道闭锁肝纤维化的治疗和预防提供了依据。
Abstract:
Objective　To investigate the action mechanism of circular RNA(circRNA) 42398 in liver fibrosis of biliary atresia by regulating the transforming growth factor-β1（TGF-β1）/Smad signaling pathway in rats.Methods　The rat hepatic stellate cell line HSC-T6 was used to construct lentivirus stable infected cell line，and the cells were divided into the negative control group,circRNA overexpression group and circRNA knockdown group according to the difference of transfected RNA.The cells in the negative control group were transfected with negative control sequence;the cells in the circRNA overexpression group were transfected with circRNA over expression sequence;the cells in the circRNA knockdown group were transfected with circRNA low expression sequence.The cell activity was detected by cell counting kit-8;the expression levels of TGF-β1，Smad2 and Smad3 mRNA were detected by real-time quantitative polymerase chain reaction; the expression levels of TGF-β1，Smad2 and Smad3 protein were detected by Western blot.Results　There were no significant difference in cell activity among the three groups(P>0.05).There were no significant difference in the relative expression levels of TGF-β1，Smad2，Smad3 mRNA and protein between the circRNA overexpression group and negative control group（P>0.05）.The relative expression levels of TGF-β1，Smad2， Smad3 mRNA and protein in the circRNA knockdown group were significantly higher than those in the negative control group（P<0.05）.Conclusion　At the cellular level of rats,circRNA 42398 can inhibit hepatic stellate cell activation by regualting the TGF-β1/Smadsignialing pathway,thus inhibiting hepatic fibrosis and which provid a new basis for the treatment and prevention of liver fibrosis in biliary atresia.

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更新日期/Last Update: 2020-11-05