[1]王会平,郜赵伟,刘　冲,等.腺苷脱氨酶抑制剂红-9-（2-羟基-3-壬烷基）腺嘌呤对白血病细胞凋亡及细胞周期的影响[J].新乡医学院学报,2020,37(10):901-905.[doi:10.7683/xxyxyxb.2020.10.001]
　WANG Huiping,GAO Zhaowei,LIU Chong,et al.Effect of adenosine deaminase inhibitor erythro-9-(2-hydroxy-3-nonyl) adenine on cell apoptosis and cell cycle of leukemia cells[J].Journal of Xinxiang Medical University,2020,37(10):901-905.[doi:10.7683/xxyxyxb.2020.10.001]
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腺苷脱氨酶抑制剂红-9-（2-羟基-3-壬烷基）腺嘌呤对白血病细胞凋亡及细胞周期的影响
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
37
期数:
2020年10
页码:
901-905
栏目:
基础研究
出版日期:
2020-10-05

文章信息/Info

Title:
Effect of adenosine deaminase inhibitor erythro-9-(2-hydroxy-3-nonyl) adenine on cell apoptosis and cell cycle of leukemia cells
作者:
王会平; 郜赵伟; 刘　冲; 李锐成; 刘　丽; 和　婷; 董　轲
（空军军医大学第二附属医院检验科，陕西　西安　710038）
Author(s):
WANG Huiping; GAO Zhaowei; LIU Chong; LI Ruicheng; LIU Li; HE Ting; DONG Ke
(Department of Clinical Laboratories，the Second Affiliated Hospital of Air Force Medical University，Xi′an 710038，Shaanxi Province，China)
关键词:
腺苷脱氨酶抑制剂; 红-9-（2-羟基-3-壬烷基）腺嘌呤; 白血病; 细胞凋亡; 细胞周期
Keywords:
adenosine deaminase inhibitor; erythro-9(2-hydroxy-3-nonyl)adenine; leukemia; cell apoptosis; cell cycle
分类号:
R733.7
DOI:
10.7683/xxyxyxb.2020.10.001
文献标志码:
A
摘要:
目的　探讨腺苷脱氨酶抑制剂-红-9-（2-羟基-3-壬烷基）腺嘌呤（EHNA）对白血病细胞凋亡及细胞周期的影响。方法　选取白血病细胞HL60、Jurkat、U937、K562及人脐静脉血管内皮细胞（HUVEC），培养至对数生长期，将细胞随机分为对照组、0.10 mmol·L-1 EHAN处理组和0.25 mmol·L-1 EHNA处理组。对照组细胞细胞采用正常培养基培养，0.10 mmol·L-1 EHAN处理组和0.25 mmol·L-1 EHNA处理组细胞分别应用含0.10、0.25 mmol·L-1EHNA的培养基培养，培养48 h后，采用流式细胞术检测各组细胞凋亡率及对照组和0.10 mmol·L-1EHNA处理组细胞周期。结果　细胞培养48 h后，0.10 mmol·L-1EHNA处理组和0.25 mmol·L-1EHNA处理组HL60、U937、K562、Jurkat细胞凋亡率均显著高于对照组 (P<0.05)； 0.10 mmol·L-1EHNA处理组HL60、K562细胞凋亡率显著高于0.25 mmol·L-1 EHNA处理组 (P<0.05)；0.10 mmol·L-1EHNA处理组与0.25 mmol·L-1EHNA处理组U937、Jurkat细胞凋亡率比较差异无统计学意义 (P>0.05)。0.10 mmol·L-1EHNA处理组、0.25 mmol·L-1EHNA 处理组和对照组HUVEC凋亡率比较差异无统计学意义(P>0.05)。0.10 mmol·L-1 EHNA处理组K562细胞和Jurkat细胞的S期细胞比例显著高于对照组 (P<0.05)，K562细胞和HUVEC细胞的G2/M期细胞比例显著低于对照组(P<0.05)； 0.10 mmol·L-1EHNA处理组与对照组HUVEC细胞的S期细胞比例及HL60、Jurkat、U937细胞的G2/M期细胞比例比较差异无统计学意义(P>0.05)。结论　EHNA处理可以在不影响血管内皮细胞的前提下杀伤多种类型的白血病细胞，为白血病治疗药物开发提供了参考。
Abstract:
Objective　To investigate the effect of adenosine deaminase inhibitor erythro-9-(2-hydroxy-3-nonyl) adenine（EHNA） on cell apoptosis and cell cycle of leukemia cells.Methods　Different types of leukemia cells (HL60，Jurkat，U937，K562) and human umbilical vein endothelial cells (HUVEC) were selected， and which were all cultured to logarithmic growth stage.The cells were randornly divided into the control group,0.10 mmol·L-1 EHNA treatment group and 0.25 mmol·L-1 EHNA treatment group.The cells in the control group were cultured in the normal culture medium,and the cells in the EHNA treatment group were cultured in the medium containing 0.10 mmol·L-1 and 0.25 mmol·L-1 EHNA.Cell apoptosis among every group was detected by flow cytometry after cell cultured for 48 h.Cell cycle was detected by flow cytometry in the control group and the 0.10 mmol·L-1EHNA treatment group.Results　After cells cultured for 48 h,the cell apoptosis rates of HL60,U937,K562 and Jurkat in the 0.10 mmol·L-1 EHNA treatment group and the 0.25 mmol·L-1 EHNA treatment group were significantly higher than those in the control group(P<0.05);the cell apoptosis rates of HL60,K562 cells in the 0.10 mmol·L-1 EHNA treatment group were significantly higher than those in the 0.25 mmol·L-1 EHNA treatment group(P< 0.05)；there was no difference in the apoptosis rate of U937,Jurkat cells between the 0.10 mmol·L-1 EHNA treatment group and the 0.25 mmol·L-1 EHNA treatment group (P>0.05) .There was no difference in cell apoptosis rate of HUVEC among the control group，0.10 mmol·L-1 EHNA treatment group and 0.25 mmol·L-1EHNA treatment group(P>0.05).The proportions of S-stage cells of K562 cells and Jurkat cells in the 0.10 mmol·L-1 EHNA treatment group were significantly higher than those in the control group(P<0.05);the proportions of G2/M-stage cells of K562 and HUVEC cells in the 0.10 mmol·L-1 EHNA treatment group were significantly lower than those in the control group(P<0.05).There was no difference in the proportion of S-stage cells of HUVEC cells and G2/M-stage cells of HL60,Jurkat and U937 between the 0.10 mmol·L-1 EHNA treatment group and the control group(P>0.05).Conclusion　EHNA treatment could induce apoptosis of leukemia cells without affecting vascular endothelial cells，which should provide a reference for the development of leukemia therapeutic drugs.

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更新日期/Last Update: 2020-10-05