[1]朱静芳,田　歌,范　威,等.不同大气颗粒物对人Ⅱ型肺泡上皮细胞的毒性效应比较[J].新乡医学院学报,2019,36(11):1007-1012.[doi:10.7683/xxyxyxb.2019.11.002]
　ZHU Jing-fang,TIAN Ge,FAN Wei,et al.Comparative study on toxicological effect of different kinds of ambient particulate matter on human typeⅡalveolar epithelial cells[J].Journal of Xinxiang Medical University,2019,36(11):1007-1012.[doi:10.7683/xxyxyxb.2019.11.002]
点击复制

不同大气颗粒物对人Ⅱ型肺泡上皮细胞的毒性效应比较
分享到：

《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
36
期数:
2019年11
页码:
1007-1012
栏目:
基础研究
出版日期:
2019-11-05

文章信息/Info

Title:
Comparative study on toxicological effect of different kinds of ambient particulate matter on human typeⅡalveolar epithelial cells
作者:
朱静芳; 田　歌; 范　威; 刘影影; 张艳格; 王　贵; 吴卫东
（新乡医学院公共卫生学院，河南省空气污染健康效应与干预国际联合实验室，河南　新乡　453003）
Author(s):
ZHU Jing-fang; TIAN Ge; FAN Wei; LIU Ying-ying; ZHANG Yan-ge; WANG Gui; WU Wei-dong
(College of Public Health,Xinxiang Medical University，International Joint Laboratory on Health Effects and Intervention of Air Pollution,，Xinxiang 453003，Henan Province，China)
关键词:
大气颗粒物; 肺泡上皮细胞; 毒性效应; 活性氧; 白细胞介素-8
Keywords:
particulate matter; alveolar epithelial cells; poisoning effect; reactive oxygen species; interleukin-8
分类号:
R122；X513
DOI:
10.7683/xxyxyxb.2019.11.002
文献标志码:
A
摘要:
目的　比较4种大气颗粒物对人Ⅱ型肺泡上皮细胞A549的毒性效应。方法　将收集的新乡市冬季大气细颗粒物（PM2.5）与常用的标准参考颗粒物（柴油机尾气颗粒物DEP-Sagai、SRM 2975及城区扬尘标准颗粒物SRM 1649）分别用磷酸盐缓冲液制备成颗粒物悬液，对人肺泡上皮细胞株A549进行24 h不同剂量染毒，采用甲基噻唑基四唑比色法、乳酸脱氢酶（LDH）测定试剂盒、活性氧（ROS）检测试剂盒及人白细胞介素-8（IL-8）酶联免疫吸附试验试剂盒分别检测细胞相对存活率、细胞培养上清液中LDH和 IL-8水平、细胞内ROS水平。结果　4种颗粒物均可致A549细胞损伤，且该效应呈剂量依赖性。低剂量SRM 1649即可明显引起细胞死亡，当剂量超过30 mg·L-1后其细胞存活率趋于稳定。4种颗粒物处理的细胞培养上清液中的LDH水平自高至低依次为SRM 2975> SRM 1649>DEP-Sagai>PM2.5，IL-8水平自高至低依次为PM2.5>DEP-Sagai>SRM 2975> SRM 1649；4种颗粒物均可不同程度地诱导A549细胞内ROS水平升高，在相同剂量下4种颗粒物诱导ROS产生的能力依次为DEP-Sagai> SRM 2975> SRM 1649>PM2.5。结论　4种大气颗粒物在受试剂量范围内均能不同程度地引起A549细胞损伤、氧化应激和炎症反应，且呈剂量效应。
Abstract:
Objective　To study the toxicological effects of different types of ambient particulate matter on human alveolar epithelial cell line A549.Methods　The suspension of PM2.5 which were collected during the winter of Xinxiang city，diesel exhaust particles including DEP-Sagai and SRM 2975，and urban dust SRM1649 were prepared by phosphate buffer solution,and then the A549 cells line were stimulated by different doses of suspension of four particulate matter for 24 hours.The relative survival rate of cells，the levels of lactate dehydrogenase (LDH)，interleukin-8 (IL-8) in cell culture supernatant and reactive oxygen species (ROS) level in cells were detected by methl thiazolyl tetrazolizum colorimetry，LDH test kit，ROS test kit and human enzyme-linked immunosorbent kit respectively.Results　All four kinds of particulate matter could cause damage to A549 cells，and the effect was dose dependent.SRM 1649 could caused cell death at low dose，and its cell survival rate stabilized when the dose exceeded 30 mg·L-1.The LDH level in the cell culture supernatants of the four particulates was SRM 2975>SRM 1649>DEP-Sagai>PM2.5，and the IL-8 level was PM2.5>DEP-Sagai>SRM 2975>SRM 1649；four kinds of particulate matter could induce the increase of ROS level in A549 cells in different extents，and the induction potencies of four kinds of particulate matter at the same dose was DEP-Sagai>SRM 2975> SRM 1649>PM2.5.Conclusion　Four kinds of particulate matter can cause damage to A549 cells within the range of reagent dosage，and it has a dose effect.

参考文献/References:

［1］　王腾飞，苏布达，姜彤.气候变化背景下的雾霾变化趋势与对策［J］.环境影响评价，2014，1（6）：15-17.
［2］　ZIELINSKA B，SAGEBIEL J，ARNOTT W P，et al.Phase and size distribution of polycyclic aromatic hydrocarbons in diesel and gasoline vehicle emissions［J］.Environ Sci Technol,2004,38(9): 2557-2567.
［3］　陈纯，朱泽军，刘丹，等.郑州市大气PM2.5的污染特征及源解析［J］.中国环境监测，2013，5（1）：47-52.
［4］　张艾蕊，王海，王梅玲，等.车用汽柴油标准物质的研究进展［J］.化学试剂，2016（10）：959-964.
［5］　黄志辉，郝春晓，王军方，等.机动车污染物排放量分析《中国机动车环境管理年报（2017）》第Ⅱ部分［J］.环境保护，2017,13（9）：42-47.
［6］　HABERT C，GARNIER R.Health effects of ambient air pollution in developing countries ［J］.Rev Mal Respir,2015,32(2):138-154.
［7］　VIEIRA J L，MACEDO F Y，BENJO A M，et al.Systemic effects of controlled exposure to diesel exhaust: a meta-analysis from randomized controlled trials［J］.Ann Med,2017,49(2):165-175.
［8］　陈分定.PMF、CMB、FA等大气颗粒物源解析模型对比研究［D］.长春：吉林大学，2011.
［9］　SHI G L，TIAN Y Z，MA T，et al.Size distribution，directional source contributions and pollution status of PM from Chengdu，China during a long-term sampling campaign［J］.J Environ Sci,2017,56:1-11.
［10］　温玲丽.城市建筑施工扬尘空间运移模式的数值模拟［D］.甘肃：兰州大学，2011.
［11］　VAN DONKELAAR A，MARTIN R V，BRAUER M，et al.Global estimates of ambient fine particulate matter concentrations from satellite-based aerosol optical depth: development and application［J］.Environ Health Perspect,2010,118(6):847-855.
［12］　周建军，乐秀芳，韩家娴，等.评价抗癌物质活性的改良MTT方法［J］.中国医药工业杂志，1993，24（10）：455-457.
［13］　GUO X，WEI H.Progress on the health effects of ambient PM2.5 pollution［J］.Chin Sci Bull (Chin Ver),2013,58(13): 1171-1177.
［14］　LAVIGNE E，YASSEEN A S，STIEB D M，et al.Ambient air pollution and adverse birth outcomes: differences by maternal comorbidities［J］.Environ Res,2016,148:457-466.
［15］　ZHANG Y，CAO S，XU X，et al.Metals compositions of indoor PM2.5，health risk assessment，and birth outcomes in Lanzhou，China［J］.Environ Monit Assess,2016,188(6):325.
［16］　COSTA L G，COLE T B，COBURN J，et al.Neurotoxicity of trafficrelated air pollution［J］.Neurotoxicology,2017,59:133-139.
［17］　COSTA L G，COLE T B，COBURN J，et al.Neurotoxicants are in the air: convergence of human，animal，and in vitro studies on the effects of air pollution on the brain［J］.Biomed Res Int,2014,2014:736385.
［18］　ANDERSON J O，THUNDIYIL J G，STOLBACH A.Clearing the air: a review of the effects of particulate matter air pollution on human health［J］.J Med Toxicol,2012,8(2):166-175.
［19］　MANNUCCI P，FRANCHINI M.Health effects of ambient air pollution in developing countries［J］.Int J Environ Res Public Health,2017,14(9):1048-1056.
［20］　LIU SK，CAI S，CHEN Y，et al.The effect of pollutional haze on pulmonary function［J］.J Thorac Dis,2016,8(1):E41-E56.
［21］　KIM H，KIM J，KIM S，et al.Cardiovascular effects of long-term exposure to air pollution: apopulation-based study with 900 845 person-years of follow-up［J］.J Am Heart Assoc, 2017,6(11):7170-7186.
［22］　GHIO A J，CARRAWAY M S，MADDEN M C.Composition of air pollution particles and oxidative stress in cells，tissues，and living systems［J］.J Toxicol Environ Health B Crit Rev,2012,15(1):1-21.
［23］　VUONG N Q，BREZNAN D，GOEGAN P，et al.In vitro toxicoproteomic analysis of A549 human lung epithelial cells exposed to urban air particulate matter and its water-soluble and insoluble fractions［J］.Part Fibre Toxicol,2017,14(1):39.
［24］　PEIXOTO M S,DE OLIVEIRA GALVA~O M F，BATISTUZZO DE MEDEIROS S R.Cell death pathways of particulate matter toxicity［J］. Chemosphere,2017,188:32-48.
［25］　SCHNEIDER J C，CARD G L，PFAU J C，et al.Air pollution particulate SRM 1648 causes oxidative stress in RAW 264.7 macrophages leading to production of prostaglandin E2，a potential Th2 mediator［J］.Inhal Toxicol,2005,17(14):871-877.
［26］　WANG L，LIU X，WU Y，et al.Involvement of ROS in the inhibitory effect of thermotherapy combined with chemotherapy on A549 human lung adenocarcinoma cell growth through the Akt pathway［J］.Oncol Rep,2012,28(4):1369-1375.
［27］　SAGAI M S H，ICHINOSE T，KODAMA M，et al.Biological effects of diesel exhaust particles.I.In vitro production of superoxide and in vivo toxicity in mouse［J］.Free Rad Biol Medicine，1993,14（1）:37-47.
［28］　VESTERDAL L K，JANTZEN K，SHEYKHZADE M，et al.Pulmonary exposure to particles from diesel exhaust，urban dust or singlewalled carbon nanotubes and oxidatively damaged DNA and vascular function in apoE(-/-) mice［J］.Nanotoxicology,2014,8(1):61-71.
［29］　YAMAGUCHI C，LEE W Y.A cost effective，sensitive，and environmentally friendly sample preparation method for determination of polycyclic aromatic hydrocarbons in solid samples［J］.J Chromatogr A,2010,1217(44):6816-6823.
［30］　AHMED T M，BERGVALL C，ABERG M，et al.Determination of oxygenated and native polycyclic aromatic hydrocarbons in urban dust and diesel particulate matter standard reference materials using pressurized liquid extraction and LC-GC/MS［J］.Anal Bioanal Chem，2015,407 (2):427-438.
［31］　MATSUO M,SHIMADA T,YAMANAKA S,et al.Diesel exhaust particle-induced cell death of human leukemic promyelocytic cells HL-60 and their variant cells HL-NR6［J］. Biol Pharm Bull, 2001,24(4):357-363.
［32］　MITSUYOSHI M，TOSHIO S,RIE U,et al.Diesel exhaust particle-induced cell death of cultured normal human bronchial epithelial cells［J］.Biol Pharm Bull,2003,26(4): 438-447.
［33］　AKHTAR U S，MCWHINNEY R D，RASTOGI N，et al.Cytotoxic and proinflammatory effects of ambient and source-related particulate matter (PM) in relation to the production of reactive oxygen species (ROS) and cytokine adsorption by particles［J］.Inhal Toxicol,2010,22(2):37-47.
［34］　KOCBACH A，TOTLANDSDAL A I，LAG M，et al.Differential binding of cytokines to environmentally relevant particles: a possible source for misinterpretation of in vitro results［J］.Toxicol Lett, 2008,176(2):131-137.

相似文献/References:

[1]樊毫军,张健鹏,刘又宁,等.递增浓度肿瘤坏死因子攻击下肺泡上皮细胞分泌IL-8水平变化[J].新乡医学院学报,2006,23(01):000.
[2]张　锐,孟玉娇,冯继伟,等.大气颗粒物暴露对呼吸道病毒感染的影响研究进展[J].新乡医学院学报,2022,39(6):593.[doi:10.7683/xxyxyxb.2022.06.019]
　ZHANG Rui,MENG Yujiao,FENG Jiwei,et al.Research progress on the effect of particulate matter exposure on respiratory virus infections[J].Journal of Xinxiang Medical University,2022,39(11):593.[doi:10.7683/xxyxyxb.2022.06.019]
[3]郭伟丽,李　航,安　珍,等.n-3多不饱和脂肪酸对心血管疾病的防治作用及其机制研究现状[J].新乡医学院学报,2016,33(3):227.[doi:10.7683/xxyxyxb.2016.03.019]
　GUO Weili,LI Hang,AN Zhen,et al.Research status of the preventive and therapeutic effects of n-3 polyunsaturated fatty acids on cardiovascular diseases and their mechanisms[J].Journal of Xinxiang Medical University,2016,33(11):227.[doi:10.7683/xxyxyxb.2016.03.019]
[4]曹胜男,杨中智.大气颗粒物源解析技术研究进展[J].新乡医学院学报,2016,33(11):1007.[doi:10.7683/xxyxyxb.2016.11.019]
　CAO Shengnan,YANG Zhongzhi.Research progress on source apportionment technology of atmospheric particulate matter[J].Journal of Xinxiang Medical University,2016,33(11):1007.[doi:10.7683/xxyxyxb.2016.11.019]

更新日期/Last Update: 2019-11-05