[1]王　位,马续祥,陈卫东.环黄芪醇对庆大霉素诱导小鼠急性肾损伤的保护作用[J].新乡医学院学报,2019,36(8):715-719.[doi:10.7683/xxyxyxb.2019.08.004]
　WANG Wei,MA Xu-xiang,CHEN Wei-dong.Protective effect of cycloastragenol on acute kidney injury induced by gentamicin in mice[J].Journal of Xinxiang Medical University,2019,36(8):715-719.[doi:10.7683/xxyxyxb.2019.08.004]
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环黄芪醇对庆大霉素诱导小鼠急性肾损伤的保护作用
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《新乡医学院学报》[ISSN:1004-7239/CN:41-1186/R]

卷:
36
期数:
2019年8
页码:
715-719
栏目:
基础研究
出版日期:
2019-08-05

文章信息/Info

Title:
Protective effect of cycloastragenol on acute kidney injury induced by gentamicin in mice
作者:
王　位; 马续祥; 陈卫东
(蚌埠医学院第一附属医院肾内科，安徽　蚌埠　233000)
Author(s):
WANG Wei; MA Xu-xiang; CHEN Wei-dong
(Department of Nephrology,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233000，Anhui Province，China)
关键词:
环黄芪醇; 急性肾损伤; 炎性因子; 抗氧化
Keywords:
cycloastragenol; acute kidney injury; inflammatory cytokines; antioxidant
分类号:
R692
DOI:
10.7683/xxyxyxb.2019.08.004
文献标志码:
A
摘要:
目的　探讨环黄芪醇对庆大霉素诱导的小鼠急性肾损伤的保护作用及其机制。方法　将30只C57BL/6J小鼠随机分为空白组、模型组及环黄芪醇低、中、高剂量组，每组6只。模型组及环黄芪醇低、中、高剂量组小鼠腹腔注射庆大霉素(100 mg·kg-1)，每日1次，连续7 d；同时，环黄芪醇低、中、高剂量组小鼠分别按200、400、800 mg·kg-1 的剂量给予环黄芪醇灌胃，每日1次，连续7 d；空白组和模型组小鼠灌胃等量生理盐水。各组小鼠均于造模第7天当晚禁食、水，造模第8天经眶内静脉采血，检测血清肌酐(CRE）、尿素氮(BUN)、还原型谷胱甘肽(GSH）、超氧化物歧化酶(SOD）和丙二醛(MDA）水平；然后采用颈椎脱臼法处死小鼠，取左侧肾脏进行苏木精-伊红染色和过碘酸希夫染色，观察各组小鼠肾组织病理学表现；取右侧肾组织制备匀浆，使用酶标仪通过比色法检测SOD、GSH、MDA、单核细胞趋化蛋白-1(MCP-1)及肿瘤坏死因子-α（TNF-α）水平。结果　与空白组比较，模型组及环黄芪醇低、中、高剂量组小鼠血清BUN、CRE、MDA 水平显著升高(P<0.05），SOD和GSH水平显著降低(P<0.05）。与模型组比较，环黄芪醇低、中、高剂量组小鼠血清 BUN、CRE、MDA水平显著降低(P<0.05），SOD、GSH水平显著升高(P<0.05）。与环黄芪醇低剂量组比较，环黄芪醇中、高剂量组小鼠血清 BUN、CRE、MDA水平显著降低(P<0.05），SOD、GSH水平显著升高(P<0.05）。与环黄芪醇中剂量组比较，环黄芪醇高剂量组小鼠血清 BUN、CRE、MDA水平显著降低(P<0.05），SOD、GSH水平显著升高(P<0.05）。与空白组比较，模型组及环黄芪醇低、中、高剂量组小鼠肾组织MDA、MCP-1、TNF-α水平显著升高(P<0.05），SOD、GSH水平显著降低(P<0.05）。与模型组比较，环黄芪醇低、中、高剂量组小鼠肾组织MDA、MCP-1、TNF-α水平显著降低(P<0.05），SOD、GSH水平显著升高(P<0.05）。与环黄芪醇低剂量组比较，环黄芪醇中、高剂量组小鼠肾组织MDA、MCP-1、TNF-α水平显著降低(P<0.05），SOD、GSH水平显著升高(P<0.05）。与环黄芪醇中剂量组比较，环黄芪醇高剂量组小鼠肾组织MDA、MCP-1、TNF-α水平显著降低(P<0.05），SOD、GSH水平显著升高(P<0.05）。结论　环黄芪醇对庆大霉素诱导的急性肾损伤具有保护作用，且呈剂量效应，其机制可能是通过改善氧化应激反应和降低炎性因子分泌而发挥治疗作用。
Abstract:
Objective　To study the protective effect and mechanism of cycloastragenol on acute kidney injury induced by gentamicin in mice.Methods　Thirty C57BL/6J mice were randomly divided into blank group,model group and low-,medium-,high-dose cycloastragenol group,6 mice in each group.The mice in the model group and low-,medium-,high-dose cycloastragenol group were injected with gentamicin (100 mg·kg-1) intraperitoneally,once a day for 7 days.Meanwhile,the mice in low-,medium-,high-dose cycloastragenol group were given 200,400 and 800 mg·kg-1 cycloastragenol respectively by intragastric administration,once a day for 7 days.The mice in the control group and model group were given equal amounts of normal saline.The mice in all group were fasted and deprived of water on the 7th day of modeling.The blood was collected from the intraorbital vein on the 8th day of modeling and the levels of creatinine (CRE),urea nitrogen (BUN),reduced glutathione (GSH),superoxide dismutase (SOD) and malondialdehyde (MDA) were measured.Then the mice were sacrificed by cervical dislocation and the kidney was taken.The tissue of left kidney was stained with haematoxylin-eosin and periodic acid-schiff and the histopathological changes were observed.The right kidney tissue was taken to prepare homogenate and the levels of SOD,GSH,MDA,monocyte chemoattractant protein-1 (MCP-1) and tumor necrosis factor-α(TNF-α) in kidney tissue were detected by colorimetric assay.Results　Compared with the blank group,the levels of BUN,CRE and MDA in serum of mice in the model group and low-,medium-,high-dose cycloalgenol group increased significantly (P<0.05);while the levels of SOD and GSH decreased significantly (P<0.05).Compared with the model group,the levels of BUN,CRE and MDA in serum of mice in the low-,medium-,high-dose cycloalgenol group decreased significantly (P<0.05);while the levels of SOD and GSH were increased significantly (P<0.05).Compared with the low-dose cycloalgenol group,the levels of BUN,CRE and MDA in serum of mice in the middle-and high-dose cycloalgenol group decreased significantly (P<0.05);while the levels of SOD and GSH increased significantly (P<0.05).Compared with the middle-dose cycloalgenol group,the levels of BUN,CRE and MDA in serum of mice in the high-dose cycloalgenol group decreased significantly (P<0.05);while the levels of SOD and GSH increased significantly (P<0.05).Compared with the blank group,the levels of MDA,MCP-1 and TNF-α in kidney tissue of mice in the model group and low-,middle- and high-dose cycloalgenol group increased significantly (P<0.05);while the levels of SOD and GSH decreased significantly (P<0.05).Compared with the model group,the levels of MDA,MCP-1 and TNF-α in kidney tissue of mice in the low-,middle- and high-dose cycloalgenol group decreased significantly (P<0.05);while the levels of SOD and GSH increased significantly (P<0.05).Compared with the low-dose cycloalgenol group,the levels of MDA,MCP-1 and TNF-α in kidney tissue of mice in the middle- and high-dose cycloalgenol group decreased significantly (P<0.05);while the levels of SOD and GSH increased significantly (P<0.05).Compared with the middle dose cycloalgenol group,the levels of MDA,MCP-1 and TNF-α in kidney tissue of mice in the high dose cycloalgenol group decreased significantly (P<0.05);while the levels of SOD and GSH increased significantly (P<0.05).Conclusion　Cycloastragalol has a dose-dependent protective effect on gentamicin-induced acute kidney injury in a dose-dependent manner.It may play therapeutic role by improving oxidative stress response and reducing the secretion of inflammatory factors.

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更新日期/Last Update: 2019-08-05